Replication FailureOpen accessMedicine (General)
Parsa Mohammadi, Elaheh Karimi, Adeleh Maleki et al. · 2022 · Acta Medica Iranica
Accumulating evidence suggests the potential use of chloroquine, an anti-malaria medication, as a neuroprotective agent. Moreover, several studies have reported that the endogenous opioids and nitric oxide (NO) may mediate the chloroquine’s effects. In the present study, effects of chloroquine on hyoscine-induced memory impairment were assessed. Furthermore, the possible involvements of opioids and NO were evaluated. Chloroquine was administered intraperitonially (i.p.) at doses of 0.1, 0.5, 1, 3, 10, and 20 mg/kg to hyoscine-treated (1mg/kg, i.p.) mice, and the spatial and fear memories were
View details →Negative / Null Result ReportMedicine
Anusuya, Balaji, Manikandan · 2026 · Journal of Indian Society of Periodontology
Periodontal regeneration aims to restore the natural structure of the periodontium, therefore improving the long-term stability and function of the affected teeth. Traditional bone graft materials such as allografts, autografts,…
View details →Replication FailureOpen accessSpecialties of internal medicine
Pooja Budhiraja, Rocio Lopez, Rocio Lopez et al. · 2025 · Transplant International
This study compares outcomes between Simultaneous Pancreas-Kidney Transplantation (SPKT) and Deceased Donor Kidney Transplantation (DDKT) in recipients with diabetes, assessing survival benefits against surgical and immunological risks. We analyzed Scientific Registry of Transplant Recipients data (2014–2023) to assess patient and kidney graft survival. Overlap propensity score weighting was applied to adjust for group differences. Kaplan-Meier and Cox proportional hazards models were used to estimate survival outcomes in unadjusted, covariate-adjusted, and weighted analyses. Among 22,545 reci
View details →Replication FailureOpen accessMedicine
Tatsuya Nagano, Masashi Hashimoto, Shintaro Izumi et al. · 2024 · Scientific Reports
Abstract Previously reported oropharyngeal exercises are long and difficult to perform. Therefore, we created a 10-min daily oropharyngeal exercise program and conducted a study to confirm its effectiveness. Twenty-five participants whose apnoea–hypopnoea index (AHI) values were greater than 5 were enrolled. All of the participants performed 10 min of exercise per day for 12 weeks and were evaluated for AHI values, tongue pressure, lip closure pressure, snoring, and Mallampati scores before and after the exercise. Twenty-two participants (88% of all participants) completed the oropharyngeal ex
View details →Methods Dead-EndOpen accessAnimal culture
Antonio Fernando Bergamaschine, Milton Passipiéri, Walter Valério Veriano Filho et al. · 2006 · Revista Brasileira de Zootecnia
Avaliaram-se a qualidade e o valor nutritivo de silagens de capim-marandu produzidas com polpa cítrica (PC) peletizada, com aditivo enzimático-bacteriano (ABE) ou com forragem emuchercida (E): T1 - forragem sem tratamento (controle); T2 - 10% de polpa cítrica peletizada (PC); T3 - aditivo enzimático bacteriano (AEB - SIL-ALL-C4 da Alltech), à base de 5 g/L de água/t de forragem; T4 - forragem picada grosseiramente emurchecida por 4 horas ao sol. O capim foi colhido aos 60 dias de rebrota (24% MS). Para avaliar o consumo e a digestibilidade das silagens, foram utilizados 16 novilhos mestiços co
View details →Failed Experiment ReportOpen accessArctic medicine. Tropical medicine
Hilton Antônio Mata-Santos, Camila Victória Sousa Oliveira, Daniel F Feijo et al. · 2024 · PLoS Neglected Tropical Diseases
Oxidative stress promotes T. cruzi growth and development of chronic Chagas heart dysfunction. However, the literature contains gaps that must be fulfilled, largely due to variations in parasite DTU sources, cell types, mouse strains, and tools to manipulate redox status. We assessed the impact of oxidative environment on parasite burden in cardiomyoblasts and the effects of the Nrf2-inducer COPP on heart function in BALB/c mice infected with either DTU-II Y or DTU-I Colombian T. cruzi strains. Treatment with antioxidants CoPP, apocynin, resveratrol, and tempol reduced parasite burden in cardi
View details →Failed Experiment ReportOpen accessNeurosciences. Biological psychiatry. Neuropsychiatry
Theo Mota, Theo Mota, Martin Giurfa et al. · 2010 · Frontiers in Behavioral Neuroscience
In multiple reversal learning, animals trained to discriminate a reinforced from a non-reinforced stimulus are subjected to various, successive reversals of stimulus contingencies (e.g. A+ vs. B-, A- vs. B+, A+ vs. B-). This protocol is useful to determine whether or not animals learn to learn and solve successive discriminations faster (or with fewer errors) with increasing reversal experience. Here we used the olfactory conditioning of proboscis extension reflex to study how honeybees Apis mellifera perform in a multiple reversal task. Our experiment contemplated four consecutive differentia
View details →Failed Experiment ReportOpen accessMedicine
Se-Hee Min, Suk Hyung Choe, Won Shik Kim et al. · 2022 · Scientific Reports
Abstract Flap failure after microvascular reconstructive surgery is a rare but devastating complication caused by reperfusion injury and tissue hypoperfusion. Remote ischemic conditioning (RIC) provides protection against ischemia/reperfusion injury and reduces tissue infarction. We hypothesized that RIC would enhance flap oxygenation and exert organ-protective effects during head and neck free flap reconstructive surgery. Adult patients undergoing free flap transfer surgery for head and neck cancer were randomized to receive either RIC or sham-RIC during surgery. RIC consisted of four cycles
View details →Negative / Null Result ReportMedicine
Ng, Chu, Chan et al. · 2026 · Journal of gastroenterology and hepatology
Management of malignant colorectal polyp has been a treatment dilemma for clinicians. This study aims to evaluate the long-term survival outcome of patients with and without salvage surgery after local excision. From January 2000 to…
View details →Failed Experiment ReportOpen accessMedicine
Luca G Valente, Ngoc Dung Le, Melissa Pitton et al. · 2022 · PLoS ONE
Background Treatment failure in pneumococcal meningitis due to antibiotic resistance is an increasing clinical challenge and alternatives to antibiotics warrant investigation. Phage-derived endolysins efficiently kill gram-positive bacteria including multi-drug resistant strains, making them attractive therapeutic candidates. The current study assessed the therapeutic potential of the novel endolysin PlyAZ3aT in an infant rat model of ceftriaxone-resistant pneumococcal meningitis. Methods Efficacy of PlyAZ3aT was assessed in a randomized, blinded and controlled experimental study in infant Wis
View details →Negative / Null Result ReportOpen accessMicrobiology
Zhuo Li, Yuan Zong, Dayu Chen et al. · 2025 · Frontiers in Cellular and Infection Microbiology
ObjectiveTo compare and analyze the efficacy and safety of different antifungal drug treatment regimens for patients with invasive pulmonary aspergillosis (IPA) in the intensive care unit (ICU).MethodsWe retrospectively collected the clinical data of patients with IPA in the ICU in two grade-A tertiary hospitals from January 2019 to January 2024 using the HIS system and compared the clinical efficacy, incidence of adverse drug reactions (ADRs), and all-cause mortality at discharge among different antifungal treatments, such as voriconazole alone, caspofungin alone, and a combination of voricon
View details →Negative / Null Result ReportOpen accessDentistry
Selin Yalnız, Hayal Boyacıoglu, Lezize Sebnem Turkun · 2026 · BMC Oral Health
Abstract Background To investigated the combined effect of adhesive type and film thickness on dentin bond strength by comparing one and two-step universal adhesives applied in single and double layers. Methods Forty caries-free human third molars were collected and standardized Class I cavities were prepared. Specimens were restored using one-step (Clearfil Tri-S Bond Universal, Japan) and two-step (G2-Bond Universal, Japan) universal adhesive systems. Each group was subdivided into single and double-layer applications, light-cured, and restored with a universal composite resin. Sticks with a
View details →Negative / Null Result ReportOpen accessSurgery
Homero Zapata-Chavira, Marco Hernández-Guedea, Julio César Jiménez-Pérez et al. · 2019 · Journal of Investigative Surgery
Aim: Remote ischemic preconditioning (RIPC) has been used as a strategy to reduce acute renal injury and ischemia-reperfusion injury (IRI) in renal transplantation (RT) with controversial results. Objective: To determine if RIPC modifies IRI in cadaveric RT recipients through inflammatory mediators and graft function. Methods: Twenty-nine RT recipients were studied, 12 in the control group (CG) and 17 in the RIPC group. RIPC which was performed on donors using a pneumatic tourniquet placed on both thighs for 10 min followed by the determination of IL-1, IL-6, TNF-α, VEGF, and ICAM-1, and hemat
View details →Negative / Null Result ReportOpen accessMedicine
Wenyun Zeng, Danbin Wu, Yingxin Sun et al. · 2021 · Scientific Reports
Abstract NLRP3 inflammasome is a vital player in macrophages pyroptosis, which is a type of proinflammatory cell-death and takes part in the pathogenesis of atherosclerosis. In this study, we used apoE−/− mice and ox-LDL induced THP-1 derived macrophages to explore the mechanisms of MCC950, a selective NLRP3 inhibitor in treating atherosclerosis. For the in vivo study, MCC950 was intraperitoneal injected to 8-week-old apoE−/− mice fed with high-fat diet for 12 weeks. For the in vitro study, THP-1 derived macrophages were treated with ox-LDL and MCC950 for 48 h. MCC950 administration reduced pl
View details →Negative / Null Result ReportMedicine
Wongtangman, Reddy Janga, Azimaraghi et al. · 2026 · Critical care (London, England)
Patients recovering from cardiac surgery in the intensive care unit (ICU) do not sleep well. Commonly used sedative-hypnotic medications can disrupt sleep architecture and increase the risk of delirium in critically ill patients after…
View details →Negative / Null Result ReportOpen accessMicrobiology
Akihiro Ito, Tadashi Ishida, Hiromasa Tachibana et al. · 2020 · Journal of Global Antimicrobial Resistance
Objectives: Nursing and healthcare-associated pneumonia (NHCAP) was proposed by the Japanese Respiratory Society to refer to healthcare-associated pneumonia. This study aimed to investigate whether antipseudomonal antibiotic therapy improved the prognosis of NHCAP patients at high risk for antimicrobial-resistant pathogens. Methods: Consecutive hospitalised NHCAP patients in Kurashiki Central Hospital between October 2010 and December 2016 were prospectively enrolled. NHCAP patients who were at high risk for antimicrobial resistance were defined as those who received antimicrobials in the prec
View details →Negative / Null Result ReportOpen accessOphthalmology
Park HM, Ryu YU, Park IJ et al. · 2020 · Clinical Optometry
Hyung Min Park, 1 Young Uk Ryu, 2 In Jee Park, 1 Byoung Sun Chu 1 1School of Optometry and Vision Science, Daegu Catholic University, Gyeongsan-si, Republic of Korea; 2Department of Physical Therapy, Daegu Catholic University, Gyeongsan-si, Republic of KoreaCorrespondence: Byoung Sun ChuSchool of Optometry and Vision Science, Daegu Catholic University, 13-13 Hayang-ro, Hayang-eup, Gyeongsan-si, Gyeongsangbuk-do 38430, Republic of KoreaTel +82-53-850-2553Fax +82-53-359-6793Email bschu@cu.ac.krBackground: The purpose of this study is to investigate the effect of wearing neutral density (ND) filt
View details →Negative / Null Result ReportOpen accessAnimal culture
K. Krag, L.L. Janss, M.M. Shariati et al. · 2013 · Animal
Heritability is a central element in quantitative genetics. New molecular markers to assess genetic variance and heritability are continually under development. The availability of molecular single nucleotide polymorphism (SNP) markers can be applied for estimation of variance components and heritability on population, where relationship information is unknown. In this study, we evaluated the capabilities of two Bayesian genomic models to estimate heritability in simulated populations. The populations comprised different family structures of either no or a limited number of relatives, a single
View details →Negative / Null Result ReportOpen accessImmunologic diseases. Allergy
Ryoko Sato, Benjamin Fintan · 2020 · Human Vaccines & Immunotherapeutics
Background: Despite the proven effectiveness of vaccinations, vaccination uptake is limited in Nigeria. According to the Multiple Indicator Cluster Survey (MICS), one of the main barriers is the lack of accurate knowledge of the vaccination schedule. This study evaluates caregivers’ knowledge of the vaccination schedule and their ability to read the immunization card. Methods: The study evaluated the knowledge of caregivers in 11 settlements in the Jada local government area of Adamawa State in September 2019. The change in knowledge among caregivers before and after referring to the immunizat
View details →Negative / Null Result ReportOpen accessBiotechnology
Alicja Hinz, Joanna Lewandowska-Łańcucka, Ewa Werner et al. · 2024 · Journal of Nanobiotechnology
Abstract Background The elastomechanical properties of nanocarriers have recently been discussed as important for the efficient delivery of various therapeutics. Some data indicate that optimal nanocarriers’ elasticity can modulate in vivo nanocarrier stability, interaction with phagocytes, and uptake by target cells. Here, we presented a study to extensively analyze the in vivo behavior of LIP-SS liposomes that were modified by forming the silicone network within the lipid bilayers to improve their elastomechanical properties. We verified liposome pharmacokinetic profiles and biodistribution,
View details →Negative / Null Result ReportOpen accessSpecial aspects of education
Rima Khasawneh, Kari Simonsen, Jessica Snowden et al. · 2016 · Medical Education Online
Background: Electronic learning allows individualized education and may improve student performance. This study assessed the impact of e-modules about infection control and congenital infections on medical knowledge. Methods: A descriptive study was conducted involving third-year medical students on pediatric clerkship. e-Module content in three different formats was developed: a text monograph, a PowerPoint presentation, and a narrated PowerPoint lecture. Students’ use of the e-modules was tracked, as was participation in the infectious disease rotation and the order of pediatric rotation. Pr
View details →Negative / Null Result ReportOpen accessPsychology
Ζωή Μπαμπλέκου, Ελισάβετ Χρυσοχόου, Σμαράγδα Καζή · 2023 · Ψυχολογία: το Περιοδικό της Ελληνικής Ψυχολογικής Εταιρείας
Despite the established interplay between executive functioning and metacognition, evidence remains limited regarding children’s metacognitive monitoring in the form of offline performance estimates of their executive efficiency. Moreover, we know little about the relations among listening comprehension, executive functions, and metalinguistic awareness in childhood. The present study constitutes a first exploration of developmental profiles related to executive functioning, listening comprehension, and corresponding metacognitive-metacomprehension monitoring in periods marking their emergence
View details →Negative / Null Result ReportOpen accessDiseases of the respiratory system
Rizna Abdul Cader, Kanagerajan Thoriappa, Rozita Mohd et al. · 2019 · Respiratory Medicine Case Reports
A 54 year old lady with underlying chronic lung disease on long term oxygen therapy and end stage renal disease of unknown aetiology on regular haemodialysis for two years started developing progressive shortness of breath during her routine haemodialysis. She was unable to tolerate her haemodialysis sessions which had to be terminated prematurely in view of her symptoms despite adjustment of her dry weight and treatment of anaemia. She was not in chronic fluid overload and her symptoms always worsened after initiation of haemodialysis and improved after termination of haemodialysis. She was a
View details →Negative / Null Result ReportOpen accessEngineering (General). Civil engineering (General)
Mohammed Hefni, Maaz A. Ali · 2021 · Advances in Civil Engineering
The effectiveness of mine backfilling depends on the properties of its constituents. The high cost of cement, which is commonly used as a binder in mine backfill, has led researchers to seek alternatives to partially replace it with other binders. This study investigated the potential to use nano-calcium carbonate (NCC) and natural pozzolans (zeolite and pumice) along with Portland cement (PC) in mine backfill. Two types of experimental samples were prepared: (1) gold tailings and silica sand to investigate the effect of NCC and (2) nickel tailings to investigate the effect of natural pozzolan
View details →Negative / Null Result ReportOpen accessMedicine (General)
Ravieh Golchoobian, Fatemeh Nabavizadeh, Mehrdad Roghani et al. · 2018 · Acta Medica Iranica
Hepatotoxicity is one of the clinically adverse effects of ecstasy (3, 4-methylenedioxymethamphetamine; MDMA) consumption. The detoxification tissue, liver, plays a central role in maintaining circulating levels of glucose and lipid. Hypoglycemia and hypotriglyceridemia have been reported due to ecstasy abuse. Ghrelin is a 28-amino-acid peptide secreted predominantly from the stomach. It has been demonstrated that ghrelin has hepatoprotective effects and is able to increase blood glucose concentration. In the current study, we explored the effect of hepatotoxic dose of MDMA and therapeutic use
View details →Negative / Null Result ReportOpen accessPublic aspects of medicine
Armando García-Guerra, Lynnette M Neufeld, Sonia Hernández-Cordero et al. · 2009 · Salud Pública de México
OBJECTIVE: The objective of this analysis was to test the impact of daily supplementation with multiple micronutrients (MM) during pregnancy on Zn, vitamin A and folate status compared to iron only (Fe). MATERIAL AND METHODS: The study was carried out during 1997-2000 in a semi-urban community in Morelos state, Mexico. Women were randomly assigned to MM (n= 249) or Fe (n= 258) and received supplements daily (6 d/wk) under supervision by the field team from recruitment (approximately 9 weeks pregnancy) until delivery. Blood samples were collected on a sub-sample of women at baseline, 32 weeks p
View details →Negative / Null Result ReportOpen accessMedicine
Tatsuya Yasuda, Tomohiko Hasegawa, Yu Yamato et al. · 2015 · Asian Spine Journal
Study DesignCase series.PurposeThe objective of this study was to investigate the change in pulmonary function in adult patients with a spinal deformity who underwent spinal corrective surgery.Overview of LiteratureDegenerative lumbar and/or thoraco-lumbar deformities are is often prominent in adult spinal deformity cases, whereas a thoracic deformity involving the chest wall is inconspicuous. A lumbar spine deformity could affect the pulmonary function; however, few reports have investigated pulmonary function in adult patients with a spinal deformity.MethodsThis study included 14 adult patie
View details →Negative / Null Result ReportOpen accessDiseases of the circulatory (Cardiovascular) system
Wioletta Sacharczuk, Rafał Dankowski, Stefan Ożegowski et al. · 2023 · Frontiers in Cardiovascular Medicine
We aimed to compare therapies of sacubitril/valsartan + spironolactone (S/V + S) with angiotensin-converting enzyme inhibitors + spironolactone (ACEI + S) on the left-sided cardiac reverse remodeling (L-CRR). The second objective was to analyze the usefulness of GLS and LVEF in response to therapy.Methods78 patients (mean age 63.4 years, 20 females) with symptomatic heart failure with reduced ejection fraction were randomized to groups of equal numbers, i.e., 39 patients, and started on therapy of S/V + S or ACEI + S. Second evaluations were made after 6–8 weeks of therapy.ResultsGLS changed f
View details →Negative / Null Result ReportOpen accessMedicine (General)
Weishen WANG, Wenhui LOU, Zhiwei XU et al. · 2023 · Journal of Advanced Research
Introduction: The value of extended lymphadenectomy in pancreatoduodenectomy (PD) has been discussed by five randomized controlled studies. However, the limitations in the studies made their conclusions not sufficiently reliable. Objectives: This multi-center randomized controlled study was designed to clarify the efficacy of extended lymphadenectomy in PD for pancreatic ductal adenocarcinoma (PDAC). Methods: From December 2016 to October 2018, 170 consecutive patients undergoing PD were enrolled and randomized to standard or extended lymphadenectomy for the treatment of PDAC from three high-v
View details →Negative / Null Result ReportOpen accessSpecial aspects of education
Maryam Amiri, Zahra Khademian, Reza Nikandish · 2018 · BMC Medical Education
Abstract Background The complexity of patients’ condition and treatment processes in intensive care units (ICUs) predisposes patients to more hazardous events. Effective patient safety culture is related to lowering the rate of patients’ complications and fewer adverse events. The present study aimed to determine the effect of empowering nurses and supervisors through an educational program on patient safety culture in adult ICUs. Methods A randomized controlled trial was conducted during April–September 2015 in 6 adult ICUs at Namazi Hospital, Shiraz, Iran. A total of 60 nurses and 20 supervi
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