Negative / Null Result ReportOpen accessMicrobiology
Yongzhi Zhang, Sen Feng, Lingyun Zhu et al. · 2023 · Frontiers in Microbiology
Zooplankton provides bacteria with a complex microhabitat richen in organic and inorganic nutrients, and the bacteria community also changes the physiochemical conditions for zooplankton, where the symbiotic relationship between them plays an important role in the nutrient cycle. However, there are few studies on the effect of associated bacteria on the population dynamics of rotifers. In order to make clear their relationships, we reconstructed the associated bacterial community in Brachionus calyciflorus culture, and examined the life history and population growth parameters, and analyzed th
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Hamideh Raeisi, Masoumeh Azimirad, Elham Abdemohamadi et al. · 2023 · Frontiers in Microbiology
IntroductionThe dramatic increase in multidrug-resistance of Clostridioides difficile isolates has led to the search for new complementary medicines against C. difficile infection (CDI). In this study, we aimed to examine the inhibitory effects of hydroethanolic extract of Mentha longifolia L. (ETOH-ML) on the growth of C. difficile RT001 and its toxigenic cell-free supernatant (Tox-S)-induced inflammation and apoptosis.MethodsThe active phytochemical components of ETOH-ML were detected using GC and HPLC. The antimicrobial properties of the extract were examined against C. difficile RT001. Fur
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Xinda Li, Xiaoyi Luo, Bin Wang et al. · 2024 · mSphere
ABSTRACT Tuberculosis (TB) remains one of the infectious diseases with high incidence and high mortality. About a quarter of the population has been latently infected with Mycobacterium tuberculosis. At present, the available TB treatment strategies have the disadvantages of too long treatment duration and serious adverse reactions. The sustained inflammatory response leads to permanent tissue damage. Unfortunately, the current selection of treatment regimens does not consider the immunomodulatory effects of various drugs. In this study, we preliminarily evaluated the effects of commonly used
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Kayla J. Ragland, Kipton B. Travis, Emmalie R. Spry et al. · 2025 · Metabolites
Background : Muscle loss during sarcopenia and atrophy is also commonly associated with age-related insulin resistance. Interestingly, branched-chain amino acids (BCAA) which are known for stimulating muscle protein synthesis are commonly elevated during insulin resistance and sarcopenic obesity. Objectives : This study investigated the effects of the interplay between atrophy and insulin resistance on insulin sensitivity, mitochondrial metabolism, and BCAA catabolic capacity in a myotube model of skeletal muscle insulin resistance. Methods : C2C12 myotubes were treated with dexamethasone to i
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Selvaraj Pavulraj, Rhett W. Stout, Daniel B. Paulsen et al. · 2023 · Viruses
Like other alpha herpesviruses, pseudorabies virus (PRV) establishes lifelong latency in trigeminal ganglionic (TG) neurons. Upon stress, the latent viruses in the TG neurons reactivate and are transported anterograde from the neuron cell bodies to the nerve endings in the nasal mucosa, where they replicate and are discharged in the nasal and oral secretions. Consequently, the virus is transmitted to other naïve animals. This cycle of latency and reactivation continues until the animal dies or is slaughtered. We have constructed a PRV triple mutant virus (PRVtmv) and used it as a live subunit
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Bakori, H. S., Kumurya, A. S., Idris, A. M. · 2024 · UMYU Journal of Microbiology Research
Mycoplasma genitalium is an emerging sexually transmitted pathogen with potential implications for reproductive health. This study aimed to investigate and compare the seroprevalence of M. genitalium among fertile and infertile women to provide insights into its potential association with fertility outcomes. A cross-sectional study was conducted involving a total of 90 women, including 31 fertile and 59 infertile women. Serum samples were collected and tested for the presence of M. genitalium-IgG antibodies using ELISA detection techniques. Data on demographic characteristics, including age an
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Mieko Higuchi‐Takeuchi, Kumiko Morisaki, Keiji Numata · 2020 · MicrobiologyOpen
Abstract Marine purple photosynthetic bacteria are ideal organisms for the production of useful materials at reduced costs and contributing to a sustainable society because they can utilize sunlight, seawater, and components of air, including carbon dioxide and nitrogen gases, for their growth. However, conjugation is the only applicable method for the transformation of marine purple photosynthetic bacteria so far. Here, we examined a calcium chloride‐mediated method for the transformation of marine purple photosynthetic bacteria. Plasmid DNAs containing the kanamycin resistance gene were succ
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Zhuo Li, Yuan Zong, Dayu Chen et al. · 2025 · Frontiers in Cellular and Infection Microbiology
ObjectiveTo compare and analyze the efficacy and safety of different antifungal drug treatment regimens for patients with invasive pulmonary aspergillosis (IPA) in the intensive care unit (ICU).MethodsWe retrospectively collected the clinical data of patients with IPA in the ICU in two grade-A tertiary hospitals from January 2019 to January 2024 using the HIS system and compared the clinical efficacy, incidence of adverse drug reactions (ADRs), and all-cause mortality at discharge among different antifungal treatments, such as voriconazole alone, caspofungin alone, and a combination of voricon
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Akihiro Ito, Tadashi Ishida, Hiromasa Tachibana et al. · 2020 · Journal of Global Antimicrobial Resistance
Objectives: Nursing and healthcare-associated pneumonia (NHCAP) was proposed by the Japanese Respiratory Society to refer to healthcare-associated pneumonia. This study aimed to investigate whether antipseudomonal antibiotic therapy improved the prognosis of NHCAP patients at high risk for antimicrobial-resistant pathogens. Methods: Consecutive hospitalised NHCAP patients in Kurashiki Central Hospital between October 2010 and December 2016 were prospectively enrolled. NHCAP patients who were at high risk for antimicrobial resistance were defined as those who received antimicrobials in the prec
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Bin Zhang, Hao Guo, Zhou Li et al. · 2025 · BMC Microbiology
Abstract Backgrounds Microbial dysbiosis in the gut and urine has been implicated in patients with benign prostatic hyperplasia (BPH) in previous studies. Additionally, emerging evidence suggests that salivary dysbiosis is associated with various diseases and oral health conditions. Building on these findings, this study aimed to investigate whether BPH patients exhibit a distinct salivary microbiome. Methods and materials We recruited a cohort of BPH patients (n = 50) and age- and BMI-matched healthy controls (HC; n = 50). Salivary samples were collected for 16S rRNA gene sequencing, and pros
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Tony H. Chang, Julie D. Pourtois, Naomi L. Haddock et al. · 2025 · mSphere
ABSTRACT Lysogenic bacteriophages can integrate their genome into the bacterial chromosome in the form of a prophage and can promote genetic transfer between bacterial strains in vitro. However, the contribution of lysogenic bacteriophages to the incidence of antimicrobial resistance (AMR) in clinical settings is poorly understood. Here, in a set of 186 clinical isolates of Pseudomonas aeruginosa collected from respiratory cultures from 82 patients with cystic fibrosis, we evaluate the links between prophage counts and both genomic and phenotypic resistance to six anti-pseudomonal antibiotics:
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José C. Segovia, Juan C. Ramírez, Paula Río et al. · 2025 · Microbiology Spectrum
ABSTRACT Two strains of the minute virus of mice (MVM) parvovirus (of the species Protoparvovirus rodent1), named i (immunosuppressive) and p (prototype), show disparate tropism and pathogenicity in mice. Unlike the virulent MVMi, the MVMp is attenuated and infection-restricted in primary mouse hematopoietic cells; however, it does infect human transformed, established, and stem cells. We thus seek here a putative MVMp natural tropism to humans compared to MVMi. Infectious purified MVMp virions suppress in vitro the clonogenic potential of human committed (erythroid and myeloid) and primitive
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Philip El-Duah, Benjamin Meyer, Augustina Sylverken et al. · 2019 · Viruses
Known human coronaviruses are believed to have originated in animals and made use of intermediate hosts for transmission to humans. The intermediate hosts of most of the human coronaviruses are known, but not for HCoV-NL63. This study aims to assess the possible role of some major domestic livestock species as intermediate hosts of HCoV-NL63. We developed a testing algorithm for high throughput screening of livestock sera with ELISA and confirmation with recombinant immunofluorescence assay testing for antibodies against HCoV-NL63 in livestock. Optimization of the ELISA showed a capability of
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Tangolar Semih, Tarım Güzin, Kelebek Haşim et al. · 2015 · BIO Web of Conferences
This study aims at assessing the effects of increased bud load and irrigation applications on berry quality of the Razakı table grape. Two Regulated Deficit Irrigation (RDI) having different irrigation levels (RDI-I and RDI-II) based on the growth stages, in addition to a non-irrigated control treatment together with two different bud load practices (K-normal and 2K-two-fold buds of the normal) were examined for their effects on quality attributes such as sugar and organic acids contents, phenolic compounds as well as antioxidant capacity of the berries. The non-irrigated vines had highest sug
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Mamta Soni, Supraja Sundaram, Sindhuja Kesavan · 2021 · National Journal of Laboratory Medicine
Introduction: Haemophagocytic Lymphohistiocytosis (HLH) is a rapidly progressive life threatening condition in which activated macrophages engulf haematopoietic cells. HLH is often overlooked because of its rarity and lack of awareness of this prevailing condition. Though previously attempts have been made to quantify hemophagocytes in marrow aspirates, to the best of present knowledge, none of the studies have correlated the number of hemophagocytes with the clinical outcomes of the patients. Aim: To study the role of laboratory parameters and number of hemophagocytes detected in bone marrow
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Binoti E., Gomes M. C., De Calais Junior A. et al. · 2016 · Helminthologia
Due to an inadequate knowledge about threats to the sea turtle, we aimed to evaluate the helminth fauna of Chelonia mydas which had died on the southern coast of Espirito Santo, Brasil and described the associated tissue pathological lesions. Retrospective and prospective studies on turtle parasites were conducted and tissues samples were collected. 106 of 212 of sea turtles (50 %) were parasitized, and 47 of 106 of infected animals 43.0 % (47/106) were in poor health condition. Seven trematoda families covering 19 different helminths species were identified. Turtles were inhabited with one or
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Wang Linlin, Song Tingting, Du Yuejiao et al. · 2024 · BIO Web of Conferences
In order to find out the change rule of SPAD value in lettuce leaves and the rapid, non-destructive and more accurate detection method of chlorophyll content in lettuce leaves under intercropping mode, the effect of intercropping on SPAD value in lettuce leaves was studied and the correlation model between chlorophyll content and SPAD value was established. The results show that the SPAD value of lettuce leaves increases at first and then decreases slowly with the development of the days of aerosol culture, and intercropping has obvious advantage in the middle and late stages of aerosol cultur
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Yuhan Xie, Gang Peng, Hongyu Zhao et al. · 2023 · Metabolites
Pregnancy at an advanced maternal age is considered a risk factor for adverse maternal, fetal, and neonatal outcomes. Here we investigated whether maternal age could be associated with differences in the blood levels of newborn screening (NBS) markers for inborn metabolic disorders on the Recommended Universal Screening Panel (RUSP). Population-level NBS data from screen-negative singleton infants were examined, which included blood metabolic markers and covariates such as age at blood collection, birth weight, gestational age, infant sex, parent-reported ethnicity, and maternal age at deliver
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Ruliaty Lisa, Amalia Indrian Rizka, Nur Abidin et al. · 2023 · BIO Web of Conferences
This study was aimed to know the effect of hatching and survival rate of milkfish larvae (Chanos chanos) treated with nanosilver. Three concentrations of nanosilver such as 0.25 ppm, 0.5 ppm and 0.75 ppm were applied to culture media at least an hour before stocking. Newly hatched larvae were stocked at the density of 50 pcs l-1, and it was reared for 25 days in 5 m3 of concrete tank. Result of the study found that no significant effect (p>0.05) of the treatments given on hatching rate and survival of milkfish larvae. However, larvae treated with nanosilver tend to gain higher hatching rate (6
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Nuiyen Aussanee, Khumin Vinta, Wichai Siriwan · 2025 · BIO Web of Conferences
Cadmium contamination found in paddy fields in the Maesot District of Tak Province, Thailand. This area was remediated using 50mg/L of ZVI. The study aimed to isolate and identify soil bacteria in the soil and rice roots and to investigate ZVI’s effect on the isolated bacterial cells. The results indicated no significant difference in soil bacteria content before and after remediation at the 95% confidence level. Twelve isolates of nitrogen-fixing bacteria were obtained. Those isolates could grow at high concentrations of 300 mg/L of ZVI. RH17 had a high tolerance for TSA with 300 mg/L of ZVI
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Miao Jiang, Pekka Kolehmainen, Laura Kakkola et al. · 2021 · Microbiology Spectrum
ABSTRACT The primary target organ of coronavirus disease 2019 (COVID-19) infection is the respiratory tract. Currently, there is limited information on the ability of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to infect and regulate innate immunity in human immune cells and lung epithelial cells. Here, we compared the ability of four Finnish isolates of SARS-CoV-2 from COVID-19 patients to replicate and induce interferons (IFNs) and other cytokines in different human cells. All isolates failed to replicate in dendritic cells, macrophages, monocytes, and lymphocytes, and no in
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Fadi Fadil, Claudia Samol, Raffaela S. Berger et al. · 2022 · Metabolites
Metabolic fingerprinting by mass spectrometry aims at the comprehensive, semiquantitative analysis of metabolites. Isotope dilution, if successfully implemented, may provide a more reliable, relative quantification. Therefore, the 13 C labeled yeast extract of the IROA TruQuant kit was added as an internal standard (IS) to human urine samples measured in full-scan mode on a high-performance liquid chromatography-time-of-flight mass spectrometer (HPLC–TOFMS) system. The isotope ratio approach enabled the analysis of 112 metabolites. The correlation with reference data did not improve significan
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Amanda Louise White, Gregory Jaye Bix · 2023 · Biomolecules
Therapeutic angiogenesis has long been considered a viable treatment for vasculature disruptions, including cerebral vasculature diseases. One widely-discussed treatment method to increase angiogenesis is vascular endothelial growth factor (VEGF) A. In animal models, treatment with VEGFA proved beneficial, resulting in increased angiogenesis, increased neuronal density, and improved outcome. However, VEGFA administration in clinical trials has thus far failed to replicate the promising results seen in animal models. The lack of beneficial effects in humans and the difficulty in medicinal trans
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Chih-Ching Yeh, Meei-Maan Wu, Chia-Min Wu et al. · 2021 · Viruses
Studies evaluating the association between age-related macular degeneration (AMD) risk and HCV infection are scant. In this population-based cohort study, 13,300 patients newly diagnosed as having HCV (HCV cohort) and 26,600 propensity score-matched patients without HCV (non-HCV cohort) were identified from the Taiwan National Health Insurance Research Database between 2000 and 2013. Furthermore, 1,983 patients with HCV who received pegylated interferon and ribavirin treatment (HCV-treated cohort) and propensity score-matched patients with HCV (matched at a ratio of 1:2) who did not receive th
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