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Browse the failure-mode index

12 real negative results, null findings, and replication failures in Biotechnology. Search the index →

WASTE indexes published research — it does not host or republish full papers. Each entry is a metadata record compiled from open scholarly databases; the abstract is shown in full only where the paper is openly licensed, otherwise a short excerpt under fair use. Classifications are automated and approximate.

Negative / Null Result ReportOpen accessBiotechnology

Occurrence and parasitism of aphids (Hemiptera: Aphididae) on cultivars of irrigated oat (Avena spp.) in São Carlos, Brazil

Júlio Cesar Ronquim, Josué Marques Pacheco, Carlos Cesar Ronquim · 2004 · Brazilian Archives of Biology and Technology

The interactions between aphids and their Hymenopteran parasitoids on irrigated oats as well as the response of different cultivars of cereals regarding the resistance to these aphids and the influence on the host/parasitoid relationships were studied during two years in São Carlos, Brazil. Rhopalosiphum padi (L.) was the predominant aphid observed throughout the study, while the other species were rarely found. Five species of parasitic Hymenoptera were found: three primary parasitoids, Lysiphlebus testaceipes (Cresson), Aphidius colemani (Viereck) and Diaeretiella rapae (M'Intosh) and two hy

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Negative / Null Result ReportOpen accessBiotechnology

A Mark-Release-Recapture Study to Estimate Field Performance of Imported Radio-Sterilized Male Aedes albopictus in Albania

Enkelejda Velo, Fabrizio Balestrino, Fabrizio Balestrino et al. · 2022 · Frontiers in Bioengineering and Biotechnology

The pathogen transmitting Aedes albopictus mosquito is spreading rapidly in Europe, putting millions of humans and animals at risk. This species is well-established in Albania since its first detection in 1979. The sterile insect technique (SIT) is increasingly gaining momentum worldwide as a component of area-wide-integrated pest management. However, estimating how the sterile males will perform in the field and the size of target populations is crucial for better decision-making, designing and elaborating appropriate SIT pilot trials, and subsequent large-scale release strategies. A mark-rel

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Negative / Null Result ReportOpen accessBiotechnology

A novel electrochemical IL-6 sensor based on Au nanoparticles-modified platinum carbon electrode

Cai Wang, Cai Wang, Dongyuan Xin et al. · 2023 · Frontiers in Bioengineering and Biotechnology

Introduction: Interleukin-6 (IL-6) is a multifunctional polypeptide cytokine composed of two glycoprotein chains, which plays an important role in many cellular reactions, pathological processes, diagnosis and treatment of diseases and so on. The detection of IL-6 plays a promising role in the cognition of clinical diseases.Methods: 4-mercaptobenzoic acid (4-MBA) was immobilized on the gold nanoparticles modified platinum carbon (PC) electrode with the linker IL-6 antibody, and finally formed an electrochemical sensor that specifically recognized IL-6. Through the highly specific antigen-antib

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Negative / Null Result ReportOpen accessBiotechnology

Impact of Eucheuma cottonii Extract on Rattus norvegicus Renal Function Test Caused by Monosodium Glutamate

Fitri Handajani, Nabil Nabil, Hanggara Rahmabrata · 2025 · Biomedical and Biotechnology Research Journal

Background: Eucheuma cottonii (EC) is a seaweed contains active compounds of alkaloids, flavonoids, saponins, and triterpenoids as antioxidants. Monosodium glutamate (MSG) is a chemical widely consumed as a flavor enhancer. The use of MSG in large amounts and for long durations triggers organ damage, including the kidneys, which is currently still a debate. This study aims to prove whether EC extract can reduce kidney damage due to MSG induction. Methods: This was an experimental animal study with probability sampling. Twenty-four Rattus norvegicus were divided into three groups: Group I witho

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Negative / Null Result ReportOpen accessBiotechnology

BMP9 attenuates microgravity-related disuse osteoporosis by modulating TGFβ and BMP signaling

Haoyang Zhao, Mengfan Yang, Yujiao Liu et al. · 2025 · npj Microgravity

Abstract Disuse osteoporosis, caused by mechanical unloading, is linked to dysregulated TGFβ and BMP signaling. This study explores their roles and evaluates BMP9 as a potential therapy. A hindlimb unloading (HLU) mouse model was used to assess bone changes and signaling alterations. In vitro, a Rotary Cell Culture System (RCCS) with 3D printing simulated microgravity. BMP9 was overexpressed in bone marrow stromal cells (BMSCs) and osteocytes treated with TGFβ1. HLU mice showed reduced bone density, microstructural integrity, and dysregulated signaling (increased p-Smad2/3, decreased p-Smad1/5

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Negative / Null Result ReportOpen accessBiotechnology

The Influence of Backpack Weight and Hip Belt Tension on Movement and Loading in the Pelvis and Lower Limbs during Walking

Katja Oberhofer, Patrick D. Wettenschwiler, Navrag Singh et al. · 2018 · Applied Bionics and Biomechanics

The introduction of hip belts to backpacks has caused a shift of loading from the spine to the hips with reported improvements in musculoskeletal comfort. Yet the effects of different hip belt tensions on gait biomechanics remain largely unknown. The goal of this study was to assess the influence of backpack weight and hip belt tension on gait biomechanics. Data from optical motion capture and ground reaction forces (GRF) during walking were acquired in nine healthy male subjects (age 28.0 ± 3.9 years). Six configurations of a commercial backpack were analyzed, that is, 15 kg, 20 kg, and 25 kg

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Negative / Null Result ReportOpen accessBiotechnology

The elasticity of silicone-stabilized liposomes has no impact on their in vivo behavior

Alicja Hinz, Joanna Lewandowska-Łańcucka, Ewa Werner et al. · 2024 · Journal of Nanobiotechnology

Abstract Background The elastomechanical properties of nanocarriers have recently been discussed as important for the efficient delivery of various therapeutics. Some data indicate that optimal nanocarriers’ elasticity can modulate in vivo nanocarrier stability, interaction with phagocytes, and uptake by target cells. Here, we presented a study to extensively analyze the in vivo behavior of LIP-SS liposomes that were modified by forming the silicone network within the lipid bilayers to improve their elastomechanical properties. We verified liposome pharmacokinetic profiles and biodistribution,

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Negative / Null Result ReportOpen accessBiotechnology

Association of SNP Rs34678647 with breast cancer risk in the Vietnamese population: An initial study

Nga Thi Nguyen, Thanh Thi Ngoc Nguyen, Hue Thi Nguyen · 2025 · Ho Chi Minh City Open University Journal of Science - Engineering and Technology

Breast cancer remains a leading cause of mortality among women worldwide. In Vietnam, the rising incidence underscores the urgent need for early diagnostic markers. Genetic factors, particularly Single Nucleotide Polymorphisms (SNPs), play a critical role in breast cancer susceptibility. The SNP rs34678647, located downstream of the miR-221/222 cluster targeting Estrogen Receptor alpha (ERα), is hypothesized to influence breast cancer risk by modulating cancer-related pathways. This study investigates the association between rs34678647 and breast cancer risk in the Vietnamese population. A tot

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Replication FailureOpen accessBiotechnology

Sequential Bone Repair in Rabbit Sinus Lifts Using Bio-Oss and Hyaluronic Acid–Polynucleotide Gel (Regenfast)

Nozomi Maniwa, Samuel Porfirio Xavier, Sergio Luis Scombatti de Souza et al. · 2024 · Journal of Functional Biomaterials

Background: A comprehensive investigation to associate the use of polynucleotides and hyaluronic acid with bovine bone in maxillary sinus lift procedures in rabbits has not been performed yet. The aim was to evaluate the influence of this novel association on the processes of bone regeneration in maxillary sinus augmentation. Methods: In this prospective, randomized, within-animal model, maxillary sinus augmentation was performed bilaterally in 12 rabbits. Deproteinized bovine bone material (DBBM) was used as filler material. A hyaluronic acid–polynucleotide gel was aggregated at the test site

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Negative / Null Result ReportOpen accessBiotechnology

Bending Characteristics of Multilayered Soft and Hardwood Materials

Tomáš Svoboda, Daniel Ruman, Milan Gaff et al. · 2015 · BioResources

The influence of selected factors, such as wood species, veneer combinations, and loading cycles, on the bending strength and modulus of elasticity of glued laminated wood was investigated after cyclic loading of 0, 1000, 3000, and 7000 cycles with European beech (Fagus sylvatica (L.)) and Eurasian aspen (Populus tremula (L.)) The laminated woods were created by a combination of densified and non-densified veneers. The 30% densification was carried out by cold rolling. All factors and their interactions had statistically significant influence on the modulus of elasticity. Similarly, the influe

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Negative / Null Result ReportOpen accessBiotechnology

Effect of different ultra-high pressure treatment on the quality of jujube wine

HUANG Weijing, CAI Wenchao, CUI Mengjun, WANG Yurong, SHAN Chunhui, GUO Zhuang · 2024 · Zhongguo niangzao

In order to investigate the effect of ultra-high pressure on the quality of jujube wine, the jujube wine prepared by Saccharomyces cerevisiae HBUAS61676 was treated at a pressure of 50-600 MPa, and the changes of physicochemical indexes, color, taste, and volatile flavor of jujube wine after different pressure treatments were studied. The results showed that ultra-high pressure treatment had no significant effect on the pH of jujube wine (P>0.05), but significantly affected the soluble solid content and the alcohol content (P<0.05). It could significantly affect the color of jujube wine (P<0.0

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Negative / Null Result ReportOpen accessBiotechnology

Clostridium acetobutylicum atpG-Knockdown Mutants Increase Extracellular pH in Batch Cultures

Yu-Sin Jang, Hyeon Jeong Seong, Seong Woo Kwon et al. · 2021 · Frontiers in Bioengineering and Biotechnology

ATPase, a key enzyme involved in energy metabolism, has not yet been well studied in Clostridium acetobutylicum. Here, we knocked down the atpG gene encoding the ATPase gamma subunit in C. acetobutylicum ATCC 824 using a mobile group II intron system and analyzed the physiological characteristics of the atpG gene knockdown mutant, 824-2866KD. Properties investigated included cell growth, glucose consumption, production of major metabolites, and extracellular pH. Interestingly, in 2-L batch fermentations, 824-2866KD showed no significant difference in metabolite biosynthesis or cell growth comp

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