Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Casey W. Dunn, Felipe Zapata, Catriona Munro et al. · 2018 · Proceedings of the National Academy of Sciences
There is considerable interest in comparing functional genomic data across species. One goal of such work is to provide an integrated understanding of genome and phenotype evolution. Most comparative functional genomic studies have relied…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Ditte Caroline Andersen, Suganya Ganesalingam, Charlotte Harken Jensen et al. · 2014 · Stem Cell Reports
The mammalian heart has generally been considered nonregenerative, but recent progress suggests that neonatal mouse hearts have a genuine capacity to regenerate following apex resection (AR). However, in this study, we performed AR or sham…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Oliver Pain, Frank Dudbridge, Alastair G. Cardno et al. · 2018 · American Journal of Medical Genetics Part B Neuropsychiatric Genetics
This study aimed to test for overlap in genetic influences between psychotic-like experience traits shown by adolescents in the community, and clinically-recognized psychiatric disorders in adulthood, specifically schizophrenia, bipolar disorder, and major depression. The full spectra of psychotic-like experience domains, both in terms of their severity and type (positive, cognitive, and negative), were assessed using self- and parent-ratings in three European community samples aged 15-19 years (Final N incl. siblings = 6,297-10,098). A mega-genome-wide association study (mega-GWAS) for each p
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Ricard Pruna, Rosa Artells, Jordi Ribas et al. · 2013 · BMC Musculoskeletal Disorders
BACKGROUND: The biological mechanisms involved in non-contact musculoskeletal soft tissue injuries (NCMSTI) are poorly understood. Genetic risk factors may be associated with susceptibility to injuries, and may exert marked influence on recovery times. METHODS: Data on type and degree of injury and recovery time were collected in 73 male professional soccer players (43 White, 11 Black Africans and 19 Hispanics) who suffered total of 242 injuries (203 muscle, 24 ligament, and 15 tendon injuries). One single nucleotide polymorphism (SNPs) in the following genes were analyzed: Elastin (ELN); Titi
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
William J. Sandborn, Scott D. Lee, Dino Tarabar et al. · 2017 · Gut
Objective This phase II, randomised, double-blind, placebo-controlled clinical trial was designed to evaluate the efficacy and safety of PF-00547659, a fully human monoclonal antibody that binds to human mucosal addressin cell adhesion…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Véronique Campbell, Pierre Legendre, François-Joseph Lapointe · 2011 · BMC Evolutionary Biology
BACKGROUND: CADM is a statistical test used to estimate the level of Congruence Among Distance Matrices. It has been shown in previous studies to have a correct rate of type I error and good power when applied to dissimilarity matrices and to ultrametric distance matrices. Contrary to most other tests of incongruence used in phylogenetic analysis, the null hypothesis of the CADM test assumes complete incongruence of the phylogenetic trees instead of congruence. In this study, we performed computer simulations to assess the type I error rate and power of the test. It was applied to additive dis
View details →Replication FailureOpen accessBiochemistry, Genetics and Molecular Biology
Karlijn Meeks, Peter Henneman, Andrea Venema et al. · 2017 · Clinical Epigenetics
Epigenome-wide association studies (EWAS) have identified DNA methylation loci involved in adiposity. However, EWAS on adiposity in sub-Saharan Africans are lacking despite the high burden of adiposity among African populations. We undertook an EWAS for anthropometric indices of adiposity among Ghanaians aiming to identify DNA methylation loci that are significantly associated. The Illumina 450k DNA methylation array was used to profile DNA methylation in whole blood samples of 547 Ghanaians from the Research on Obesity and Diabetes among African Migrants (RODAM) study. Differentially methylat
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Paul E. Harrington, Kaustav Biswas, David J. Malwitz et al. · 2014 · ACS Medicinal Chemistry Letters
The kinase/endonuclease inositol requiring enzyme 1 (IRE1α), one of the sensors of unfolded protein accumulation in the endoplasmic reticulum that triggers the unfolded protein response (UPR), has been investigated as an anticancer target.…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Miguel Martín, Aleix Prat, Álvaro Rodríguez-Lescure et al. · 2013 · Breast Cancer Research and Treatment
To identify a group of patients who might benefit from the addition of weekly paclitaxel to conventional anthracycline-containing chemotherapy as adjuvant therapy of node-positive operable breast cancer. The predictive value of PAM50…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Li He, Guoying Zhou, Junang Liu et al. · 2016 · PLoS ONE
The filamentous fungus Colletotrichum fructicola is found in all five continents and is capable of causing severe diseases in a number of economically important plants such as avocado, fig, cocoa, pear, and tea-oil trees. However, almost nothing is known about its patterns of genetic variation and epidemiology on any of its host plant species. Here we analyzed 167 isolates of C. fructicola obtained from the leaves of tea-oil tree Camellia oleifera at 15 plantations in seven Chinese provinces. Multilocus sequence typing was conducted for all isolates based on DNA sequences at fragments of four
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Christian Garm, María Moreno‐Villanueva, Alexander Bürkle et al. · 2012 · Aging Cell
Exogenous and endogenous damage to DNA is constantly challenging the stability of our genome. This DNA damage increase the frequency of errors in DNA replication, thus causing point mutations or chromosomal rearrangements and has been…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Gal Avital, Mor Buchshtav, Ilia Zhidkov et al. · 2012 · Human Molecular Genetics
Heteroplasmy, the mixture of mitochondrial genomes (mtDNA), varies among individuals and cells. Heteroplasmy levels alter the penetrance of pathological mtDNA mutations, and the susceptibility to age-related diseases such as Parkinson's…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Carrie Wright, Anandita Rajpurohit, Emily E. Burke et al. · 2019 · BMC Genomics
BACKGROUND: RNA sequencing offers advantages over other quantification methods for microRNA (miRNA), yet numerous biases make reliable quantification challenging. Previous evaluations of these biases have focused on adapter ligation bias with limited evaluation of reverse transcription bias or amplification bias. Furthermore, evaluations of the quantification of isomiRs (miRNA isoforms) or the influence of starting amount on performance have been very limited. No study had yet evaluated the quantification of isomiRs of altered length or compared the consistency of results derived from multiple
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Barbara C. Sorkin, Adam J. Kuszak, Gregory Bloss et al. · 2019 · The FASEB Journal
While great interest in health effects of natural product (NP) including dietary supplements and foods persists, promising preclinical NP research is not consistently translating into actionable clinical trial (CT) outcomes. Generally…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Satoshi Ishikawa, Hiromitsu Hayashi, Kouichi Kinoshita et al. · 2013 · International Journal of Cancer
While statin intake has been proven to reduce the risk of colorectal cancer (CRC), the mechanism of antitumor effects and clinical significance in survival benefits remain unclear. Statin-induced antiproliferative effects and its…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Heath D. Skinner, Chen Hu, Theodoros Tsakiridis et al. · 2021 · JAMA Oncology
IMPORTANCE: Non-small cell lung cancer (NSCLC) has relatively poor outcomes. Metformin has significant data supporting its use as an antineoplastic agent. OBJECTIVE: To compare chemoradiation alone vs chemoradiation and metformin in stage…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Seung Joo Kang, Charleston W. K. Chiang, C. D. Palmer et al. · 2010 · Human Molecular Genetics
Genome-wide association (GWA) studies have identified common variants that are associated with a variety of traits and diseases, but most studies have been performed in European-derived populations. Here, we describe the first genome-wide…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Davide Fiore Bavaro, Alessandra Belati, Lucia Diella et al. · 2021 · Antibiotics
Cefiderocol is a new cephalosporin displaying against extensively resistant (XDR) Gram-negative bacteria. We report our experience with cefiderocol-based combination therapies as “rescue” treatments in immunocompromised or critically ill patients or in patients with post-surgical infections who had failed previous regimens. A total of 13 patients were treated from 1 September 2020 to 31 March 2021. In total, 5/13 (38%) patients were classified as critically ill, due to severe COVID-19 lung failure; 4/13 (31%) patients had post-surgical infections and 4/13 (31%) had severe infections in immunoc
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Elizabeth Nance, Siva P. Kambhampati, Elizabeth S. Smith et al. · 2017 · Journal of Neuroinflammation
Rett syndrome (RTT) is a pervasive developmental disorder that is progressive and has no effective cure. Immune dysregulation, oxidative stress, and excess glutamate in the brain mediated by glial dysfunction have been implicated in the pathogenesis and worsening of symptoms of RTT. In this study, we investigated a new nanotherapeutic approach to target glia for attenuation of brain inflammation/injury both in vitro and in vivo using a Mecp2-null mouse model of Rett syndrome. To determine whether inflammation and immune dysregulation were potential targets for dendrimer-based therapeutics in R
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
R. Anand, Kartik Shah, Hengyao Niu et al. · 2011 · Nucleic Acids Research
DNA sequences that form secondary structures or bind protein complexes are known barriers to replication and potential inducers of genome instability. In order to determine which helicases facilitate DNA replication across these barriers,…
View details →Failed Experiment ReportOpen accessBiochemistry, Genetics and Molecular Biology
Tim B. Bigdeli, Stephan Ripke, Roseann E. Peterson et al. · 2017 · Translational Psychiatry
Abstract Major depressive disorder (MDD) is a common, complex psychiatric disorder and a leading cause of disability worldwide. Despite twin studies indicating its modest heritability (~30–40%), extensive heterogeneity and a complex genetic architecture have complicated efforts to detect associated genetic risk variants. We combined single-nucleotide polymorphism (SNP) summary statistics from the CONVERGE and PGC studies of MDD, representing 10 502 Chinese (5282 cases and 5220 controls) and 18 663 European (9447 cases and 9215 controls) subjects. We determined the fraction of SNPs displaying c
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Harold Bae, Paola Sebastiani, Jenny X. Sun et al. · 2013 · Frontiers in Genetics
Personality traits have been shown to be associated with longevity and healthy aging. In order to discover novel genetic modifiers associated with personality traits as related with longevity, we performed a genome-wide association study (GWAS) on personality factors assessed by NEO-five-factor inventory in individuals enrolled in the Long Life Family Study (LLFS), a study of 583 families (N up to 4595) with clustering for longevity in the United States and Denmark. Three SNPs, in almost perfect LD, associated with agreeableness reached genome-wide significance (p < 10(-8)) and replicated in a
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Federico C. F. Calboli, Federica Tozzi, N. W. Galwey et al. · 2010 · PLoS ONE
Neuroticism is a moderately heritable personality trait considered to be a risk factor for developing major depression, anxiety disorders and dementia. We performed a genome-wide association study in 2,235 participants drawn from a population-based study of neuroticism, making this the largest association study for neuroticism to date. Neuroticism was measured by the Eysenck Personality Questionnaire. After Quality Control, we analysed 430,000 autosomal SNPs together with an additional 1.2 million SNPs imputed with high quality from the Hap Map CEU samples. We found a very small effect of popu
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Qilong Lu, Sebahattin Çirak, Terence A. Partridge · 2014 · Molecular Therapy — Nucleic Acids
On 20 September 2013, GlaxoSmithKline (GSK) and Prosensa announced that GSK's Phase III clinical trial (NCT01254019) of Drisapersen, an exon skipping drug for Duchenne muscular dystrophy (DMD), failed to meet the primary endpoint of a…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Nathan Pankratz, Alexandra Dumitriu, Kurt N. Hetrick et al. · 2011 · PLoS ONE
Copy number variants (CNVs) are known to cause Mendelian forms of Parkinson disease (PD), most notably in SNCA and PARK2. PARK2 has a recessive mode of inheritance; however, recent evidence demonstrates that a single CNV in PARK2 (but not a single missense mutation) may increase risk for PD. We recently performed a genome-wide association study for PD that excluded individuals known to have either a LRRK2 mutation or two PARK2 mutations. Data from the Illumina370Duo arrays were re-clustered using only white individuals with high quality intensity data, and CNV calls were made using two algorit
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Bria M. Coates, Kelly L. Staricha, Nandini Ravindran et al. · 2017 · Frontiers in Immunology
Influenza A virus (IAV) is a significant cause of life-threatening lower respiratory tract infections in children. Antiviral therapy is the mainstay of treatment, but its effectiveness in this age group has been questioned. In addition, damage inflicted on the lungs by the immune response to the virus may be as important to the development of severe lung injury during IAV infection as the cytotoxic effects of the virus itself. A crucial step in the immune response to IAV is activation of the NOD-like receptor protein 3 (NLRP3) inflammasome and the subsequent secretion of the inflammatory cytok
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Larry D. Mesner, Veena Valsakumar, Neerja Karnani et al. · 2010 · Genome Research
We have used a novel bubble-trapping procedure to construct nearly pure and comprehensive human origin libraries from early S- and log-phase HeLa cells, and from log-phase GM06990, a karyotypically normal lymphoblastoid cell line. When…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Jantina Toxopeus, Vladimı́r Košťál, Brent J. Sinclair · 2019 · Proceedings of the Royal Society B Biological Sciences
Freeze tolerance, the ability to survive internal ice formation, facilitates survival of some insects in cold habitats. Low-molecular-weight cryoprotectants such as sugars, polyols and amino acids are hypothesized to facilitate freeze…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
T. Dassopoulos, Marla C. Dubinsky, J. L. Bentsen et al. · 2013 · Alimentary Pharmacology & Therapeutics
BACKGROUND: Azathioprine (AZA), a pro-drug metabolised to the active metabolites 6-tioguanine nucleotides (6TGN), is a steroid-sparing therapy for Crohn's disease (CD). AIM: To investigate whether AZA therapy is optimised by individualised…
View details →Negative / Null Result ReportBiochemistry, Genetics and Molecular Biology
Eli Ben‐Chetrit, Avraham Ben‐Chetrit, Yackov Berkun et al. · 2010 · Arthritis Care & Research
OBJECTIVE: To evaluate the outcome of pregnancies in women with familial Mediterranean fever (FMF) who are taking colchicine, and to reconsider the justification for amniocentesis in these women. METHODS: The outcome of 179 pregnancies in…
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