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Browse the failure-mode index

21,229 real negative results, null findings, and replication failures. Search the index →

WASTE indexes published research — it does not host or republish full papers. Each entry is a metadata record compiled from open scholarly databases; the abstract is shown in full only where the paper is openly licensed, otherwise a short excerpt under fair use. Classifications are automated and approximate.

Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Excess diacylglycerol at the endoplasmic reticulum disrupts endomembrane homeostasis and autophagy

Dan Li, Shu-Gao Yang, Cheng-Wen He et al. · 2020 · BMC Biology

BACKGROUND: When stressed, eukaryotic cells produce triacylglycerol (TAG) to store nutrients and mobilize autophagy to combat internal damage. We and others previously reported that in yeast, elimination of TAG synthesizing enzymes inhibits autophagy under nitrogen starvation, yet the underlying mechanism has remained elusive. RESULTS: Here, we show that disruption of TAG synthesis led to diacylglycerol (DAG) accumulation and its relocation from the vacuolar membrane to the endoplasmic reticulum (ER). We further show that, beyond autophagy, ER-accumulated DAG caused severe defects in the endom

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Negative / Null Result ReportOpen accessMedicine

Centromedian Nucleus of the Thalamus Deep Brain Stimulation for Genetic Generalized Epilepsy: A Case Report and Review of Literature

Shruti Agashe, David B. Burkholder, Keith Starnes et al. · 2022 · Frontiers in Human Neuroscience

There is a paucity of treatment options for cognitively normal individuals with drug resistant genetic generalized epilepsy (GGE). Centromedian nucleus of the thalamus (CM) deep brain stimulation (DBS) may be a viable treatment for GGE. Here, we present the case of a 27-year-old cognitively normal woman with drug resistant GGE, with childhood onset. Seizure semiology are absence seizures and generalized onset tonic clonic (GTC) seizures. At baseline she had 4-8 GTC seizures per month and weekly absence seizures despite three antiseizure medications and vagus nerve stimulation. A multidisciplin

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Negative / Null Result ReportOpen accessMedicine

Tumor regression mediated by oncogene withdrawal or erlotinib stimulates infiltration of inflammatory immune cells in EGFR mutant lung tumors

Deborah Ayeni, Braden Miller, Alexandra Kuhlmann et al. · 2019 · Journal for ImmunoTherapy of Cancer

BACKGROUND: Epidermal Growth Factor Receptor (EGFR) tyrosine kinase inhibitors (TKIs) like erlotinib are effective for treating patients with EGFR mutant lung cancer; however, drug resistance inevitably emerges. Approaches to combine immunotherapies and targeted therapies to overcome or delay drug resistance have been hindered by limited knowledge of the effect of erlotinib on tumor-infiltrating immune cells. METHODS: Using mouse models, we studied the immunological profile of mutant EGFR-driven lung tumors before and after erlotinib treatment. RESULTS: We found that erlotinib triggered the re

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Negative / Null Result ReportOpen accessMedicine

Prognostic factors of BRAF V600E colorectal cancer with liver metastases: a retrospective multicentric study

Sahir Javed, Stéphane Benoist, Patrick Devos et al. · 2022 · World Journal of Surgical Oncology

BACKGROUND: BRAF V600E-mutant colorectal cancers (CRCs) are associated with shorter survival than BRAF wild-type tumors. Therapeutic decision-making for colorectal liver metastases (CRLM) harboring this mutation remains difficult due to the scarce literature. The aim was to study a large cohort of BRAF V600E-mutant CRLM patients in order to see if surgery extend overall survival among others prognostic factors. METHODS: BRAF V600E-mutant CRCs diagnosed with liver-only metastases, resected or not, were retrospectively identified between April 2008 and December 2017, in 25 French centers. Clinic

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Negative / Null Result Report

Unmasking the potential of the angiotensin AT 2 receptor as a therapeutic target in hypertension in men and women: What we know and what we still need to find out

Lucinda M Hilliard, Katrina M Mirabito, Kate M Denton · 2013 · Clinical and Experimental Pharmacology and Physiology

Summary Major sex differences exist in the development and progression of hypertension and cardiovascular disease. Prior to menopause, women have lower arterial pressure and, furthermore, are protected from hypertension and cardiovascular…

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Negative / Null Result ReportOpen accessMedicine

Evaluation of outcome and prognostic factors in patients of glioblastoma multiforme: A single institution experience

Narendra Kumar, Pankaj Kumar, Shabab Angurana et al. · 2013 · Journal of Neurosciences in Rural Practice

AIMS: We present retrospective analysis of patients of glioblastoma multiforme (GBM) and discuss clinical characteristics, various treatment protocols, survival outcomes, and prognostic factors influencing survival. MATERIALS AND METHODS:…

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Negative / Null Result ReportOpen accessMedicine

Significant differences when using creatinine, modification of diet in renal disease, or cystatin C for estimating glomerular filtration rate in ICU patients

Miklós Lipcsey, Mia Furebring, Sten Rubertsson et al. · 2010 · Upsala Journal of Medical Sciences

BACKGROUND: Renal dysfunction is associated with increased morbidity and mortality in intensive care patients. In most cases the glomerular filtration rate (GFR) is estimated based on serum creatinine and the Modification of Diet in Renal Disease (MDRD) formula, but cystatin C-estimated GFR is being used increasingly. The aim of this study was to compare creatinine and MDRD and cystatin C-estimated GFR in intensive care patients. METHODS: Retrospective observational study was performed, on patients treated within the general intensive care unit (ICU) during 2004-2006, in a Swedish university h

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Teplizumab in Type 1 Diabetes Mellitus: An Updated Review

Simran Thakkar, Aditi Chopra, Lakshmi Nagendra et al. · 2023 · touchREVIEWS in Endocrinology

Type 1 diabetes mellitus (T1DM) is a chronic autoimmune condition characterized by the irreversible destruction of the β cells of the pancreas, which leads to a lifelong dependency on exogenous insulin. Despite the advancements in insulin…

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Negative / Null Result ReportOpen accessMedicine

The use of a proposed updated EARL harmonization of 18F-FDG PET-CT in patients with lymphoma yields significant differences in Deauville score compared with current EARL recommendations

John Ly, David Minarik, Lars Edenbrandt et al. · 2019 · EJNMMI Research

The Deauville score (DS) is a clinical tool, based on the comparison between lesion and reference organ uptake of 18F-fluorodeoxyglucose (FDG), used to stratify patients with lymphoma into categories reflecting their disease status. With a plethora of positron emission tomography with computed tomography (PET-CT) hard- and software algorithms, standard uptake value (SUV) in lesions and reference organs may differ which affects DS classification and therefore medical treatment. The EANM Research Ltd. (EARL) harmonization program from the European Association of Nuclear Medicine (EANM) partly mi

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Negative / Null Result ReportOpen accessMedicine

A Phase 2 clinical trial of PF-05212377 (SAM-760) in subjects with mild to moderate Alzheimer’s disease with existing neuropsychiatric symptoms on a stable daily dose of donepezil

Terence Fullerton, Brendon Binneman, William David et al. · 2018 · Alzheimer s Research & Therapy

Symptomatic benefits have been reported for 5-HT6 receptor antagonists in Alzheimer’s disease (AD) trials. SAM-760 is a potent and selective 5-HT6 receptor antagonist that has demonstrated central 5-HT6 receptor saturation in humans at a dose of 30 mg. This was a randomized, double-blind, placebo-controlled, parallel-group, multicenter trial evaluating the efficacy and safety of SAM-760 30 mg once daily (QD) for 12 weeks in subjects with AD on a stable regimen of donepezil 5 to 10 mg QD. The study included an interim analysis with stopping rules for futility or efficacy after 180 subjects comp

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Negative / Null Result ReportOpen accessMedicine

Ultrasound guidance for femoral venous access in patients undergoing pulmonary vein isolation: A quasi‐randomized study

Péter Kupó, T Riesz, László Sághy et al. · 2023 · Journal of Cardiovascular Electrophysiology

INTRODUCTION: Routine ultrasound (US) guidance for femoral venous access to decrease vascular complications of atrial fibrillation (AF) ablation procedures has been advocated. However, the benefit has not been unequivocally demonstrated by randomized-trial data. METHODS: Consecutive patients undergoing pulmonary vein isolation (PVI) on uninterrupted anticoagulant treatment were included. A quasi-random allocation to either US-guided or conventional puncture group was based on which of the two procedure rooms the patient was scheduled in, with only one of the rooms equipped with a US machine in

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Negative / Null Result ReportOpen accessSocial Sciences

Associations between social media use and cognitive abilities: Results from a large-scale study of adolescents

Stefan Stieger, Sabine Wunderl · 2022 · Computers in Human Behavior

In adolescence, smartphone use in general and social media use in particular has often been associated with negative effects, such as higher anxiety levels and body dissatisfaction. Other outcomes – such as fundamental cognitive abilities and skills (e.g., intelligence, information processing, spatial perception) – have rarely been the focus of research. Here, we analysed data from a large sample of adolescents (12–16 years; N > 12,000) who performed a series of psychometric tests ranging from intelligence, spatial perception, and information processing, to practical numeracy, and compared the

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Negative / Null Result ReportMedicine

Significant association between TNFAIP3 inactivation and biased immunoglobulin heavy chain variable region 4‐34 usage in mucosa‐associated lymphoid tissue lymphoma

Sarah Moody, Leire Escudero‐Ibarz, Ming Wang et al. · 2017 · The Journal of Pathology

Both antigenic drive and genetic change play critical roles in the development of mucosa-associated lymphoid tissue (MALT) lymphoma, but neither alone is sufficient for malignant transformation, and lymphoma development critically depends…

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Negative / Null Result ReportOpen accessMedicine

Evaluation of a Computer-Tailored Healthy Ageing Intervention to Promote Physical Activity among Single Older Adults with a Chronic Disease

Janet M. Boekhout, Brenda Berendsen, Denise A. Peels et al. · 2018 · International Journal of Environmental Research and Public Health

This study explores the effectiveness of the Active Plus65 intervention designed to stimulate physical activity among single older adults with a chronic physical impairment. A quasi-experimental pre-test post-test study was performed. The intervention group (n = 411; mean age = 76.75; SD = 7.75) was assessed at baseline, three months, and six months. Data of comparable older adults who completed the original Active Plus intervention served as reference group (n = 87; mean age = 74.36; SD = 6.26). Multilevel regression analyses were applied: outcome measures were weekly minutes of moderate to v

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Negative / Null Result ReportOpen accessMedicine

Significant association of the dupA gene of Helicobacter pylori with duodenal ulcer development in a South-east Indian population

Jawed Alam, Sankar Maiti, Prachetash Ghosh et al. · 2012 · Journal of Medical Microbiology

A novel virulence factor, duodenal ulcer-promoting gene A (dupA), in Helicobacter pylori has been found to be associated with disease in certain populations but not in others. This study analysed a South-east Indian population as part of…

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Negative / Null Result Report

How to Use SNP_TATA_Comparator to Find a Significant Change in Gene Expression Caused by the Regulatory SNP of This Gene’s Promoter via a Change in Affinity of the TATA-Binding Protein for This Promoter

Mikhail Ponomarenko, Dmitry Rasskazov, Olga Arkova et al. · 2015 · BioMed Research International

The use of biomedical SNP markers of diseases can improve effectiveness of treatment. Genotyping of patients with subsequent searching for SNPs more frequent than in norm is the only commonly accepted method for identification of SNP…

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Negative / Null Result Report

Mobile‐bearing prosthesis did not improve mid‐term clinical results of total knee arthroplasty

Shuichi Matsuda, Hideki Mizu‐uchi, Shingo Fukagawa et al. · 2010 · Knee Surgery, Sports Traumatology, Arthroscopy

Abstract A prospective study was performed to compare the clinical and radiological results of mobile‐ and fixed‐bearing total knee arthroplasty with specific attention to rotational alignment and range of motion. Sixty‐one knees were…

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Negative / Null Result ReportOpen accessNursing

Dietary Management of Blood Glucose in Medical Critically Ill Overweight and Obese Patients: An Open‐Label Randomized Trial

Todd W. Rice, D. Clark Files, Peter E. Morris et al. · 2018 · Journal of Parenteral and Enteral Nutrition

BACKGROUND: Enteral nutrition (EN) increases hyperglycemia due to high carbohydrate concentrations while providing insufficient protein. The study tested whether an EN formula with very high-protein- and low-carbohydrate-facilitated…

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Negative / Null Result ReportOpen accessMedicine

ADCC responses and blocking of EGFR-mediated signaling and cell growth by combining the anti-EGFR antibodies imgatuzumab and cetuximab in NSCLC cells

Arjan Kol, Anton Terwisscha van Scheltinga, Martin Pool et al. · 2017 · Oncotarget

// Arjan Kol 1 , Anton Terwisscha van Scheltinga 2 , Martin Pool 1 , Christian Gerdes 3 , Elisabeth de Vries 1 , Steven de Jong 1 1 Department of Medical Oncology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands 2 Department of Clinical Pharmacy and Pharmacology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands 3 Roche Pharma Research & Early Development, Roche Innovation Center Zürich, Schlieren, Switzerland Correspondence to: Steven de Jong, email: s.de.jong@umcg.nl Keywords: non-small cell lung cancer,

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Negative / Null Result ReportMedicine

Moderate Running and Plyometric Training During Off-Season Did Not Show a Significant Difference on Soccer-Related High-Intensity Performances Compared with No-Training Controls

Daisuke Nakamura, Tomohiro Suzuki, Mikinobu Yasumatsu et al. · 2011 · The Journal of Strength and Conditioning Research

Several investigators have reported the effects of reduced training and interrupted training on athletic performance, but few reports are available for soccer players. The purpose of this study was to examine, using the Yo-Yo intermittent…

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Mechanism of the activation step of the aminoacylation reaction: a significant difference between class I and class II synthetases

Sindrila Dutta Banik, Nilashis Nandi · 2012 · Journal of Biomolecular Structure and Dynamics

In the present work we report, for the first time, a novel difference in the molecular mechanism of the activation step of aminoacylation reaction between the class I and class II aminoacyl tRNA synthetases (aaRSs). The observed difference is in the mode of nucleophilic attack by the oxygen atom of the carboxylic group of the substrate amino acid (AA) to the αP atom of adenosine triphosphate (ATP). The syn oxygen atom of the carboxylic group attacks the α-phosphorous atom (αP) of ATP in all class I aaRSs (except TrpRS) investigated, while the anti oxygen atom attacks in the case of class II aa

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