Negative / Null Result ReportOpen accessBiotechnology
Haoyang Zhao, Mengfan Yang, Yujiao Liu et al. · 2025 · npj Microgravity
Abstract Disuse osteoporosis, caused by mechanical unloading, is linked to dysregulated TGFβ and BMP signaling. This study explores their roles and evaluates BMP9 as a potential therapy. A hindlimb unloading (HLU) mouse model was used to assess bone changes and signaling alterations. In vitro, a Rotary Cell Culture System (RCCS) with 3D printing simulated microgravity. BMP9 was overexpressed in bone marrow stromal cells (BMSCs) and osteocytes treated with TGFβ1. HLU mice showed reduced bone density, microstructural integrity, and dysregulated signaling (increased p-Smad2/3, decreased p-Smad1/5
View details →Negative / Null Result ReportOpen accessNeurosciences. Biological psychiatry. Neuropsychiatry
Daniel Wiswede, Jascha Rüsseler · 2021 · Frontiers in Human Neuroscience
Introduction: The present study was conducted to verify a promising experimental setup which demonstrated an inversed Stroop-effect (much faster responses for incongruent relative to congruent Stroop trials) following a mismatching tone. In the matching condition, which was an almost exact replication of the original study, participants were required to indicate whether word color and word meaning were matching, whereas in the response conflict condition, instruction was the same as in a classical Stroop task and required the participants to respond to the word color. As in the original study,
View details →Methods Dead-EndOpen accessMedicine (General)
Di Benedetto M, Greenwood KM, Isaac F · 2012 · Patient Preference and Adherence
Fadia Isaac1, Kenneth Mark Greenwood2, Mirella Di Benedetto31Cairnmillar Institute School of Psychology Counselling and Psychotherapy, Camberwell, Victoria, Australia; 2School of Psychology and Social Science Faculty of Computing, Health and Science, Edith Cowan University, Joondalup, Western Australia, Australia; 3School of Health Sciences, Royal Melbourne Institute of Technology University, Bundoora, Victoria, AustraliaBackground: Individuals’ attitudes towards depression and its treatments may influence their likelihood of seeking professional help and adherence to treatment when depr
View details →Negative / Null Result ReportOpen accessMedicine
Cherubino Paolo, Sala Andrea, Casalone Rosario et al. · 2005 · Journal of Translational Medicine
Abstract Background Local myogenesis, neoangiogenesis and homing of progenitor cells from the bone marrow appear to contribute to repair of the infarcted myocardium. Implantation into heart tissues of autologous skeletal myoblasts has been associated with improved contractile function in animal models and in humans with acute myocardial ischemia. Since heart infarction is most prevalent in individuals of over 40 years of age, we tested whether culture methods available in our laboratory were adequate to obtain sufficient numbers of differentiated skeletal myoblasts from muscle biopsy specimens
View details →Negative / Null Result ReportOpen accessPsychology
A. Norvilas, Miller J. C. · 2010 · Psichologija
D. Fernandez-Duque ir I. M. Thornton (2000) tariamai atrado, kad asmuo gali užfiksuoti pakitimą ir be sąmonės įsiterpimo. Tačiau šį atradimą gerokai menkina vienas pačių autorių pripažintas metodologinis trūkumas – kontrolinių bandymų nebuvimas. Siekdami tiksliau nustatyti, ar norint pastebėti pakitimą reikia sąmonės tarpininkavimo, ar jo užfiksavimas gali vykti ir be sąmonės įsiterpimo, dar kartą tyrėme, kaip matomame objekte aptinkamas pasikeitimas, tačiau šį kartą tyrėmė dvejopai: vienu atveju be kontrolinių bandymų, kaip ir D. Fernandez-Duque ir I. M. Thorntonas, o kitu atveju atlikome ir
View details →Negative / Null Result ReportOpen accessInternal medicine
Elisabeth A. Rosenthal, David R. Crosslin, Adam S. Gordon et al. · 2021 · BMC Medical Genomics
Abstract Background Elevated triglycerides (TG) are associated with, and may be causal for, cardiovascular disease (CVD), and co-morbidities such as type II diabetes and metabolic syndrome. Pathogenic variants in APOA5 and APOC3 as well as risk SNVs in other genes [APOE (rs429358, rs7412), APOA1/C3/A4/A5 gene cluster (rs964184), INSR (rs7248104), CETP (rs7205804), GCKR (rs1260326)] have been shown to affect TG levels. Knowledge of genetic causes for elevated TG may lead to early intervention and targeted treatment for CVD. We previously identified linkage and association of a rare, highly cons
View details →Negative / Null Result ReportOpen accessImmunologic diseases. Allergy
Zhou Tao, Han Yanxing, Dang Ying et al. · 2009 · Retrovirology
Abstract Background Isolation of novel retroviral restriction factors will open new avenues for anti-HIV/AIDS treatment. Although HIV-1 replication is restricted by APOBEC3G/APOBEC3F, TRIM5α, and CD317, none defend HIV-1 infection under natural conditions. Previously, we demonstrated a host factor from the human T cell line CEM.NKR that potently restricted wild-type HIV-1 replication. Interestingly, this restriction resembled the APOBEC3G/APOBEC3F pattern in that viral replication was inhibited from the second round of replication cycle at a post-entry step. Results Here, we further characteri
View details →Negative / Null Result ReportOpen accessMedicine
Marta Schirripa, Wu Zhang, Dongyun Yang et al. · 2018 · PLoS ONE
BACKGROUND:Macrophages play a crucial role in the interaction between tumor and immune system, and iNOS is known as a surrogate marker of M1 macrophages activation. The goal of the study was to investigate the role of iNOS polymorphisms as prognostic marker in mCRC patients. MATERIALS AND METHODS:Functional significant polymorphisms in the promoter of INOS gene were analyzed by PCR-based and direct DNA sequencing in 4 cohorts of patients receiving bevacizumab based first-line chemotherapy: two evaluation cohorts (TRIBE ARM A and ARM B) and two validation cohorts (FIRE 3 arm A and MOMA). The re
View details →Negative / Null Result ReportOpen accessNeurosciences. Biological psychiatry. Neuropsychiatry
Megan Polden, Trevor J. Crawford · 2022 · Brain Sciences
It has been reported that performing bilateral eye movements for a short period can lead to an enhancement of memory retrieval and recall (termed the “saccade induced retrieval effect (SIRE)”). The source of this effect has been debated within the literature and the phenomenon has come under scrutiny as the robustness of the effect has recently been questioned. To date investigations of SIRE have largely been restricted to younger adult populations. Here, across two experiments, we assess the robustness and generalisability of the SIRE specifically in relation to disease and ageing. Experiment
View details →Negative / Null Result ReportOpen accessMedicine
Nicholas P. Carruth, Jairo A. Ramos, Akira Miyake · 2023 · PLoS ONE
This article reports a preregistered study in which we attempted to replicate the results of an influential study on the ego-depletion effect reported by Job, Dweck, and Walton in 2010. The original Job et al. study (Study 1, N = 60) provided evidence that the ego-depletion effect—a performance decrease on a self-control task after performing another self-control task—occurs only for individuals who hold a belief that their willpower is limited. This moderation of the ego-depletion effect by one’s willpower mindset (limited vs. nonlimited) has been interpreted as evidence against a prevalent l
View details →Negative / Null Result ReportOpen accessMotor vehicles. Aeronautics. Astronautics
Azadeh DinparastDjadid, John D. Lee, Chris Schwarz et al. · 2018 · International Journal of Automotive Engineering
Vehicles with SAE Level 2 or 3 automation rely on the driver to intervene and resume control when failures occur. In cases which the driver must steer upon regaining control, the initial conditions of the vehicle’s state variables can affect the success of the drivers' recovery. Hence, a model to determine the consequences of these initial states could help identify the requirements of shared control to guarantee a smooth recovery after an automation failure. Such a modeling tool should be simple, such as a two-point visual continuous control model of steering. Data to validate such a model we
View details →Negative / Null Result ReportOpen accessMedicine
Danni A. Gadd, Hannah M. Smith, Donncha Mullin et al. · 2024 · Clinical Epigenetics
Abstract Background Plasma growth differentiation factor 15 (GDF15) and N‐terminal proB‐type natriuretic peptide (NT‐proBNP) are cardiovascular biomarkers that associate with a range of diseases. Epigenetic scores (EpiScores) for GDF15 and NT-proBNP may provide new routes for risk stratification. Results In the Generation Scotland cohort (N ≥ 16,963), GDF15 levels were associated with incident dementia, ischaemic stroke and type 2 diabetes, whereas NT-proBNP levels were associated with incident ischaemic heart disease, ischaemic stroke and type 2 diabetes (all PFDR 95%) with levels of GDF15 an
View details →Negative / Null Result ReportOpen accessPhysiology
Mahmoud A. Mohammad, Inka C. Didelija, Barbara Stoll et al. · 2020 · Physiological Reports
Abstract Background Age‐dependent changes in the intestinal gene expression of enzymes involved in the metabolism of citrulline and arginine are well characterized. Enteroids, a novel ex‐vivo model that recreates the three‐dimensional structure of the intestinal crypt‐villus unit, have shown to replicate molecular and physiological profiles of the intestinal segment from where they originated (“location memory”). Objective The present study tested the hypothesis that enteroids recapitulate the developmental changes observed in vivo regarding citrulline production in pigs (“developmental memory
View details →Negative / Null Result ReportOpen accessInternal medicine
Banasik Karina, Hollensted Mette, Andersson Ehm et al. · 2012 · BMC Medical Genetics
Abstract Background Variations within the FOXA family have been studied for a putative contribution to the risk of type 2 diabetes (T2D), and recently the minor T-allele of FOXA2 rs1209523 was reported to associate with decreased fasting plasma glucose levels in a study using a weighted false discovery rate control procedure to enhance the statistical power of genome wide association studies in detecting associations between low-frequency variants and a given trait. Thus, the primary aim of this study was to investigate whether the minor T-allele of rs1205923 in FOXA2 associated with 1) decrea
View details →Negative / Null Result ReportOpen accessPsychology
Aiste DIRZYTE, Aidas PERMINAS, Aiste SKARNULYTE et al. · 2024 · European Journal of Mental Health
Introduction: While personality traits and early maladaptive schemas (EMSs) can affect an individual’s behavior and well-being, the links between these constructs are under-researched, especially in non-clinical samples. Aims: Two studies were conducted to address these links, intending to explore their specifics, as previous research evidenced various associations’ models. Methods: In Study 1, the sample consisted of 120 respondents (65.0% females) living in the UK and the USA. In Study 2, the sample consisted of 244 respondents (68.0% females) living in Lithuania. In both studies, most of th
View details →Negative / Null Result ReportOpen accessNeurosciences. Biological psychiatry. Neuropsychiatry
Nikola Jajcay, David Tomeček, Iveta Fajnerová et al. · 2026 · Frontiers in Computational Neuroscience
An increasing number of studies are currently focusing on “personality neuroscience,” a term denoting the research aimed at neuroimaging correlates of inter-individual temperament and character variability. Among other methods, a graph theoretical analysis of the functional connectivity in resting-state functional magnetic resonance imaging data was applied in a study by, reporting novel functional connectivity correlates of personality traits. The current paper presents a conceptual replication of the results of this study and discusses the related challenges, including an extension of the or
View details →Negative / Null Result ReportOpen accessMedicine
Kim JS · 2026 · Journal of exercise rehabilitation
This study analyzed the effects of an 8-week Pilates exercise program on health-related physical fitness and spinal morphology in female college students. As a result of performing 8 weeks of Pilates exercise program, there was a…
View details →Negative / Null Result ReportOpen accessMedicine
Muhammad Muaaz Aslam, Peter John, Kang-Hsien Fan et al. · 2019 · BMC Research Notes
Abstract Objective Type 1 diabetes (T1D) and rheumatoid arthritis (RA) are autoimmune diseases. It is known that certain genetic loci and factors that increase the overall autoimmunity risk can be shared among different autoimmune diseases. We sought to replicate seven T1D-related SNPs (single nucleotide polymorphisms) that have been previously reported to be associated with RA susceptibility in a small set of mixed family-based and case–control Pakistani sample in a relatively large and independent RA case–control sample from the same population. Seven T1D-associated SNPs (GLIS3/rs7020673, BA
View details →Negative / Null Result ReportOpen accessPsychiatry
Jonas Weijers, Jonas Weijers, Wolfgang Viechtbauer et al. · 2018 · Frontiers in Psychiatry
A previous study observed that reported childhood abuse moderated psychotic and emotional reactivity to stress among patients with non-affective psychotic disorder. However, that study used a type of analysis unsuited for skewed data. This study aimed (1) to replicate the study and (2) to examine whether we would obtain similar results using a statistical approach better suited to skewed data. Fifty-nine patients with non-affective psychotic disorder were examined for up to 6 days using an intensive diary method to assess levels of negative affect, psychosis, and daily-life stress. A mixed-lin
View details →Negative / Null Result ReportOpen accessDentistry
Damla Erkal, Turgut Felek, Oana-Paula Butean et al. · 2025 · BMC Oral Health
Abstract Introduction This study hypothesized that large language models (LLMs) would underperform compared to expert clinicians in diagnosing and managing complex endodontic anomalies, such as dens invaginatus, when provided with periapical radiographs. Although LLMs have shown promise in dental education and basic diagnostics, their effectiveness in nuanced clinical reasoning has remained unclear. Methods Nineteen anonymized periapical radiographs depicting challenging endodontic conditions were paired with clinical vignettes. Six advanced LLMs and one expert endodontist independently answer
View details →Negative / Null Result ReportOpen accessBiology (General)
Sahaana Chandran, Jan Vinjé, Kimberly Huynh et al. · 2026 · Applied and Environmental Microbiology
ABSTRACT Human intestinal enteroid (HIE) and zebrafish larva (ZF) models support the replication of certain strains of human norovirus (HuNoV), the leading cause of viral gastroenteritis worldwide. The replication of 17 HuNoV-positive stool specimens (nine genotypes) from patients ranging from 5 months to 83 years old was evaluated in both HIE and ZF models. The yolk of 3-day post-fertilization ZF larvae was microinjected with 3 nL 10% clarified stool suspension. Each day post-infection (dpi), 10 larvae were pooled as one sample, and two specimens were collected daily until 5 dpi. Viral RNA wa
View details →Negative / Null Result ReportOpen accessMedicine
Yonggang Lu, Robert John Aitken, Minjie Lin · 2017 · PLoS ONE
Galeolaria gemineoa is a sessile broadcast-spawning marine invertebrate, whose spermatozoa have been regarded as a sensitive indicator for water quality monitoring. In this study, 10 steps of spermiogenesis have been identified at the ultrastructural level and this differentiation process has been recapitulated in vitro up to the point of spermiogenesis (step 7-9 spermatids). On completion of the second meiosis, newly formed spermatids were detached from the seminiferous epithelium and released to the lumen of each germinal chamber. These spermatids were present in pairs and interconnected by
View details →Negative / Null Result ReportOpen accessScience
Andrew M. Colman, Briony D. Pulford, Caren A. Frosch et al. · 2022 · Royal Society Open Science
In this preregistered study, we attempted to replicate and substantially extend a frequently cited experiment by Schurr and Ritov, published in 2016, suggesting that winners of pairwise competitions are more likely than others to steal money in subsequent games of chance against different opponents, possibly because of an enhanced sense of entitlement among competition winners. A replication seemed desirable because of the relevance of the effect to dishonesty in everyday life, the apparent counterintuitivity of the effect, possible problems and anomalies in the original study, and above all t
View details →Negative / Null Result ReportOpen accessConsciousness. Cognition
Chiara Gambi, Jaroslaw R. Lelonkiewicz, Davide Crepaldi · 2024 · Journal of Cognition
Influential theories and computational models suggest error-based learning plays an important role in language acquisition: Children learn new words by generating predictions about upcoming utterances and revising those predictions when they are erroneous. Critically, revising stronger (rather than weaker) predictions should further enhance learning. Although previously demonstrated in adults, such prediction error boost has not been conclusively shown in children. To close this gap, we tested 107 participants between the ages of 5 and 10. We found little evidence that word learning in this ag
View details →Negative / Null Result ReportOpen accessMicrobiology
Selvaraj Pavulraj, Rhett W. Stout, Daniel B. Paulsen et al. · 2023 · Viruses
Like other alpha herpesviruses, pseudorabies virus (PRV) establishes lifelong latency in trigeminal ganglionic (TG) neurons. Upon stress, the latent viruses in the TG neurons reactivate and are transported anterograde from the neuron cell bodies to the nerve endings in the nasal mucosa, where they replicate and are discharged in the nasal and oral secretions. Consequently, the virus is transmitted to other naïve animals. This cycle of latency and reactivation continues until the animal dies or is slaughtered. We have constructed a PRV triple mutant virus (PRVtmv) and used it as a live subunit
View details →Negative / Null Result ReportOpen accessMedicine (General)
Carlo Biz, Luca Puce, Maamer Slimani et al. · 2022 · Medicina
Background and Objectives: Table tennis represents one of the fastest ball games in the world and, as such, is characterized by unique physiological demands. Despite its popularity, there is a dearth of data related to table-tennis-related risk factors and injuries. Therefore, the present review was conducted to fill in this gap of knowledge. Material and Methods: The present review was designed as a scoping review. Eleven online databases were searched with no language/date limitations. Results: Forty-two investigations were retained in the present review. These studies indicated that tenosyn
View details →Negative / Null Result ReportOpen accessTherapeutics. Pharmacology
Mudit Agarwal, Piyush Ranjan, Upendra Baitha et al. · 2020 · Frontiers in Pharmacology
Hydroxychloroquine has gained much attention as one of the candidate drugs that can be repurposed as a prophylactic agent against SARS-CoV-2, the agent responsible for the COVID-19 pandemic. Due to high transmissibility and presence of asymptomatic carriers and presymptomatic transmission, there is need for a chemoprophylactic agent to protect the high-risk population. In this review, we dissect the currently available evidence on hydroxychloroquine prophylaxis from a clinical and pharmacological point of view. In vitro studies on Vero cells show that hydroxychloroquine effectively inhibits SA
View details →Negative / Null Result ReportOpen accessZoology
Masahiro Sasaki, Hinano Kinoshita, Akitsugu Konno · 2025 · Animal Cognition
Abstract The present study explored whether a well-socialized Steller sea lion named Hama could reproduce similar actions with human demonstrations using the “Do as I do” (DAID) paradigm. Hama had learned 50 types of behaviors, but her social learning ability was unknown. In Study 1, we trained Hama to produce simultaneous DAID responses. After introducing the DAID training, we conducted four tests to confirm Hama’s acquisition of demonstrator-matching behavior. We found that Hama successfully acquired the action-matching ability for human actions, not only with three trained actions but also
View details →Negative / Null Result ReportOpen accessNeurosciences. Biological psychiatry. Neuropsychiatry
Louise Simeonov, Ravi Das · 2025 · Frontiers in Behavioral Neuroscience
A fundamental question in cognitive neuroscience is how multi-sensory elements are bound into a unified memory trace. The formation of memories is thought to be reliant on precisely timed neural activity. Theta frequency neural oscillations have been proposed to orchestrate the timing of different sensory cortices. Here, we attempt to replicate findings that flickering stimuli presented in synchronous theta frequency will lead to enhanced recall. Additionally, we investigate whether theta frequency sensory flicker can improve encoding of emotional associative memories. The current study failed
View details →Negative / Null Result ReportOpen accessAgriculture
Leila Sahampoor, Fatemeh Zaker Tavallaie, Seyed Reza Fani et al. · 2020 · Journal of Crop Protection
Trichoderma is very important as a bio control agent and probably a good alternative for chemical fungicides. Fusarium oxysporum is a plant pathogen that causes wilt in a wide range of plants. The use of gamma irradiation can be employed to increase Trichoderma efficiency against F. oxysporum. The induced mutation provides genetic changes in Trichoderma and in some of the mutated isolates the efficiency of bio control may be improved. In this study the efficiency of mutated T. harzianum CS5 against the F. oxysporum f.sp. radicis-cucumerinum was evaluated. Among 16 wild isolates of Trichoderma,
View details →