Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
M Hayat, Wenlong Carl Chen, Chantal Babb de Villiers et al. · 2025 · Nature Communications
Abstract Genome-wide association studies (GWAS) have characterized the contribution of common variants to breast cancer (BC) risk in populations of European ancestry, however GWAS have not been reported in resident African populations. This GWAS included 2485 resident African BC cases and 1101 population matched controls. Two risk loci were identified, located between UNC13C and RAB27A on chromosome 15 (rs7181788, p = 1.01 × 10 −08 ) and in USP22 on chromosome 17 (rs899342, p = 4.62 × 10 −08 ). Several genome-wide significant signals were also detected in hormone receptor subtype analysis. The
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Celia Sequera, Margherita Grattarola, Floriane Cannet et al. · 2025 · Nature Communications
Histone deacetylases (HDACs) are epigenetic regulators frequently altered in cancer. Here we report that overexpression of HDAC1/2 occurs in Hepatocellular Carcinoma (HCC) patients, correlating with poor prognosis. We show that romidepsin,…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Maria Nikogհosyan, Siras Hakobyan, Anahit Hovhannisyan et al. · 2019 · Frontiers in Genetics
Background: During last decades a number of genome-wide association studies (GWAS) has identified numerous single nucleotide polymorphisms (SNPs) associated with different complex diseases. However, associations reported in one population are often conflicting and did not replicate when studied in other populations. One of the reasons could be that most of GWAS employ case-control design in one or a limited number of populations, but little attention was paid to global distribution of disease associated alleles across different populations. Moreover, the majority of GWAS have been performed on
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Stella Aslibekyan, Hassan S. Dashti, Toshiko Tanaka et al. · 2014 · Chronobiology International
Sunlight exposure has been shown to alter DNA methylation patterns across several human cell-types, including T-lymphocytes. Since epigenetic changes establish gene expression profiles, changes in DNA methylation induced by sunlight…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Yukun Kuang, Weiping Tan, Chaohui Hu et al. · 2024 · Frontiers in Cellular and Infection Microbiology
Background: Targeted next-generation sequencing (tNGS) has become a trending tool in the field of infection diagnosis, but concerns are also raising about its performance compared with metagenomic next-generation sequencing (mNGS). This study aims to explore the clinical feasibility of a tNGS panel for respiratory tract infection diagnosis and compare it with mNGS in the same cohort of inpatients. Methods: 180 bronchoalveolar lavage fluid samples were collected and sent to two centers for mNGS and tNGS blinded tests, respectively. The concordance between pathogen reports of both methods and th
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Guangfu Hu, Guangxia Hu, Chengjiao Zhang et al. · 2020 · BMC Cancer
BACKGROUND: The benefit of adjuvant chemotherapy in invasive lobular carcinoma (ILC) is still unclear. The objective of the current study was to elucidate the effectiveness of adjuvant chemotherapy in hormone receptor (HR)-positive, human epidermal growth factor receptor 2 (HER2)-negative, pT1b-c/N0-1/M0 ILC. METHODS: Based on Surveillance, Epidemiology, and End-Results (SEER) database, we identified original 12,334 HR-positive, HER2-negative, pT1b-c/N0-1/M0 ILC patients, who were then divided into adjuvant chemotherapy group and control group. End-points were overall survival (OS) and breast
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Burooj Ghani, Vincent J. Kalkman, Bob Planqué et al. · 2025 · Scientific Reports
Animal sounds can be recognised automatically by machine learning, and this has an important role to play in biodiversity monitoring. Yet despite increasingly impressive capabilities, bioacoustic species classifiers still exhibit imbalanced performance across species and habitats, especially in complex soundscapes. In this study, we explore the effectiveness of transfer learning in large-scale bird sound classification across various conditions, including single- and multi-label scenarios, and across different model architectures such as CNNs and Transformers. Our experiments demonstrate that
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Ignacio J. Melero‐Jiménez, Yael Sorokin, Ami Merlin et al. · 2025 · Nature Communications
Populations facing lethal environmental change can escape extinction through rapid genetic adaptation, a process known as evolutionary rescue. Despite extensive study, evolutionary rescue is largely unexplored in mutualistic communities, where it is likely constrained by the less adaptable partner. Here, we explored empirically the likelihood, population dynamics, and genetic mechanisms underpinning evolutionary rescue in an obligate mutualism involving cross-feeding of amino acids between auxotrophic Escherichia coli strains. We found that over 80% of populations overcame a severe decline whe
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Leah Zuroff, Vista Farkhondeh, Riley Bove et al. · 2025 · Drugs
Despite major advances in multiple sclerosis (MS) treatment, disability accumulation independent of relapse activity remains a significant challenge. Chronic demyelination is a key driver of neurodegeneration and disease progression,…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Marlee R. Labroo, Jessica Rutkoski · 2022 · BMC Genomics
BACKGROUND: Recurrent selection is a foundational breeding method for quantitative trait improvement. It typically features rapid breeding cycles that can lead to high rates of genetic gain. Usually, generations are discrete in recurrent selection, which means that breeding candidates are evaluated and considered for selection for only one cycle. Alternately, generations can overlap, with breeding candidates considered for selection as parents for multiple cycles. With recurrent genomic selection but not phenotypic selection, candidates can be re-evaluated by using genomic estimated breeding v
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Alexander A. Rashin, Marcin J. Domagalski, Michael T. Zimmermann et al. · 2014 · Acta Crystallographica Section D Biological Crystallography
Validation of general ideas about the origins of conformational differences in proteins is critical in order to arrive at meaningful functional insights. Here, principal component analysis (PCA) and distance difference matrices are used to…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Miruna C. Barbu, Carmen Amador, Alex S. F. Kwong et al. · 2022 · EBioMedicine
BACKGROUND: DNA methylation (DNAm) is associated with time-varying environmental factors that contribute to major depressive disorder (MDD) risk. We sought to test whether DNAm signatures of lifestyle and biochemical factors were associated with MDD to reveal dynamic biomarkers of MDD risk that may be amenable to lifestyle interventions. METHODS: , N=565), using CpG sites reported in previous well-powered methylome-wide association studies. We also compared their predictive accuracy for MDD to a MDD MS in an independent GS sub-sample (N=4,432). FINDINGS: =-0.069-0.083) remained significantly a
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Mathieu Quinodoz, Kim Rodenburg, Zuzana Cvačková et al. · 2026 · Nature Genetics
Small nuclear RNAs (snRNAs) combine with specific proteins to generate small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. U4 snRNA forms a duplex with U6 and, together with U5, contributes to the tri-snRNP…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Valeriy Timofeyev, C. A. Porter, Dipika Tuteja et al. · 2010 · American Journal of Physiology-Heart and Circulatory Physiology
Adenylyl cyclase (AC) is the principal effector molecule in the β-adrenergic receptor pathway. AC(V) and AC(VI) are the two predominant isoforms in mammalian cardiac myocytes. The disparate roles among AC isoforms in cardiac hypertrophy…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Dingya Sun, Jialu Wang, Xin Li et al. · 2026 · Antioxidants
Curcumin, a natural polyphenol derived from turmeric, functions as a potent exogenous antioxidant and exhibits a range of benefits in the prevention and management of metabolic diseases. Despite its extremely low systemic bioavailability, curcumin demonstrates significant bioactivity in vivo, a phenomenon likely attributable to its accumulation in the intestines and subsequent modulation of systemic oxidative stress and inflammation. This article systematically reviews the comprehensive regulatory effects of curcumin on systemic metabolic networks-including glucose metabolism, amino acid metab
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Zhuoxu Gu, Zhonghao Wang, Xianquan Zhang et al. · 2025 · Frontiers in Pharmacology
As a modern dosage form of traditional Chinese medicine, Traditional Chinese Medicine Formula Granules (TCMFG) maximally retains active metabolites through standardized production processes, including dynamic countercurrent extraction and low-temperature concentration. This serves as a critical material basis for elucidating its multi-target regulatory mechanisms. Recent studies have highlighted the significant potential of TCMFG in treating complex diseases, such as inflammation, tumors, metabolic disorders, fibrosis, and orthopedic conditions like osteoarthritis and osteoporosis, by modulati
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Fanzhuo Xu, Yu Xie, Weiwei Yu et al. · 2026 · Frontiers in Microbiology
Multidrug resistance in Gram-negative bacteria has become a significant global public health challenge, threatening human health and clinical treatment outcomes. The unique outer membrane structure of these pathogens greatly limits antibiotic penetration, serving as the core mechanism of resistance. This paper systematically analyses antimicrobial strategies targeting the outer membrane of Gram-negative bacteria, mainly including: (1) directly disrupting the outer membrane structure and enhancing drug permeability; (2) inhibiting the biosynthesis or transport pathways of key outer membrane com
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Ornella Zollo, Neal Sondheimer · 2017 · Transcription
In vitro studies of mitochondrial transcription often use linear templates that fail to replicate key features of transcription on a circular genome. We developed a plasmid-based system for the analysis of heavy-strand promoters that…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Hiroki Isono, Taiju Miyagami, Kohta Katayama et al. · 2016 · Internal Medicine
Tetanus is a potentially fatal infection. Approximately 100 cases are reported in Japan each year; however, little is known about its clinical course and outcomes in the current era of treatment. We herein report three cases of tetanus in…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Kristin Köppen, Diana Fatykhova, Gudrun Holland et al. · 2023 · Frontiers in Cellular and Infection Microbiology
Introduction Tularemia is mainly caused by Francisella tularensis ( Ft ) subsp. tularensis ( Ftt ) and Ft subsp. holarctica ( Ftt ) in humans and in more than 200 animal species including rabbits and hares. Human clinical manifestations depend on the route of infection and range from flu-like symptoms to severe pneumonia with a mortality rate up to 60% without treatment. So far, only 2D cell culture and animal models are used to study Francisella virulence , but the gained results are transferable to human infections only to a certain extent. Method In this study, we firstly established an ex
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Vasilios Liapis, Aneta Zysk, Mark DeNichilo et al. · 2017 · Cancer Medicine
Tumor hypoxia is a major cause of treatment failure for a variety of malignancies. However, hypoxia also leads to treatment opportunities as demonstrated by the development of compounds that target regions of hypoxia within tumors. Evofosfamide is a hypoxia-activated prodrug that is created by linking the hypoxia-seeking 2-nitroimidazole moiety to the cytotoxic bromo-isophosphoramide mustard (Br-IPM). When evofosfamide is delivered to hypoxic regions of tumors, the DNA cross-linking toxin, Br-IPM, is released leading to cell death. This study assessed the anticancer efficacy of evofosfamide in
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Robert Massey, Yu Chen, Marina Panova‐Noeva et al. · 2024 · Cardiovascular Diabetology
BACKGROUND: BMI variability has been associated with increased cardiovascular disease risk in individuals with type 2 diabetes, however comparison between clinical studies and real-world observational evidence has been lacking. Furthermore, it is not known whether BMI variability has an effect independent of HbA1c variability. METHODS: We investigated the association between BMI variability and 3P-MACE risk in the Harmony Outcomes trial (n = 9198), and further analysed placebo arms of REWIND (n = 4440) and EMPA-REG OUTCOME (n = 2333) trials, followed by real-world data from the Tayside Bioreso
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Luyuan Chang, Yang Liu, Haipeng Li et al. · 2025 · Frontiers in Cellular and Infection Microbiology
Cirrhosis remains a significant global health burden, causing approximately 1.4-1.5 million deaths each year and contributing to nearly 46 million disability-adjusted life years (DALYs) worldwide. Increasing evidence identifies the gut-liver axis as a central driver of disease progression, wherein intestinal dysbiosis, barrier disruption, and microbe-derived metabolites collectively exacerbate inflammation, fibrogenesis, and related complications. Across more than 40 recent studies, gut microbial α-diversity declined by 30-60%, and over 80% reported a marked depletion of short-chain fatty acid
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Gabriele Meyer, Jeffrey R. Leipprandt, Jianwei Xie et al. · 2012 · Endocrinology
Mammary organoids from adult mice produce tubules, analogous to mammary ducts in vivo, in response to hepatocyte growth factor (HGF) when cultured in collagen gels. The combination of HGF plus progestin (R5020) causes reduced tubule number…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Jennifer Zou, Jinjing Zhou, Sarah Faller et al. · 2022 · G3 Genes Genomes Genetics
Genome-wide association studies (GWAS) have identified thousands of genetic variants associated with complex human traits, but only a fraction of variants identified in discovery studies achieve significance in replication studies. Replication in genome-wide association studies has been well-studied in the context of Winner's Curse, which is the inflation of effect size estimates for significant variants due to statistical chance. However, Winner's Curse is often not sufficient to explain lack of replication. Another reason why studies fail to replicate is that there are fundamental difference
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Jeanne M. McCaffery, George D. Papandonatos, Lucy F. Faulconbridge et al. · 2015 · Psychosomatic Medicine
OBJECTIVES: Numerous studies have found elevated depressive symptoms among individuals with Type 2 diabetes, yet the mechanisms remain unclear. We examined whether genetic loci previously associated with depressive symptoms predict…
View details →Replication FailureOpen accessBiochemistry, Genetics and Molecular Biology
Dennis Poel, Elske C. Gootjes, Lotte Bakkerus et al. · 2020 · Cancer Medicine
BACKGROUND: Palliative systemic therapy is currently standard of care for patients with extensive metastatic colorectal cancer (mCRC). A biomarker predicting chemotherapy benefit which prevents toxicity from ineffective treatment is urgently needed. Therefore, a previously developed tissue-derived microRNA profile to predict clinical benefit from chemotherapy was evaluated in tissue biopsies and serum from patients with mCRC. METHODS: Samples were prospectively collected from patients (N = 132) who were treated with capecitabine or 5-FU/LV with oxaliplatin ± bevacizumab. Response evaluation wa
View details →Negative / Null Result ReportBiochemistry, Genetics and Molecular Biology
Peipei Ma, Mogens Sandø Lund, Xiangdong Ding et al. · 2014 · Journal of Animal Breeding and Genetics
This study investigated the effect of including Nordic Holsteins in the reference population on the imputation accuracy and prediction accuracy for Chinese Holsteins. The data used in this study include 85 Chinese Holstein bulls genotyped…
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Giacomo Mutti, Eduard Ocaña‐Pallarès, Toni Gabaldón · 2025 · Molecular Biology and Evolution
Recent developments in protein structure prediction have allowed the use of this previously limited source of information at genome-wide scales. It has been proposed that the use of structural information may offer advantages over…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Jayeshkumar Kanani · 2025 · The Egyptian Journal of Neurosurgery : the official publication of the Egyptian Society of Neurological Surgeons/Egyptian journal of neurosurgery
Abstract Background Hemangioblastomas are rare, slow-growing benign neoplasms predominantly affecting the central nervous system (CNS). Despite their benign nature, they pose diagnostic challenges due to their diverse clinical manifestations and radiological features. Case presentation. A 40-year-old male presented with chronic headaches and diplopia persisting for 8 years. Initial MRI revealed two cystic lesions in the cerebellum, causing compression and herniation. Surgical removal of one cyst yielded inconclusive results. Follow-up MRIs showed cyst growth and syrinx development. The patient
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