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Browse the failure-mode index

137 real negative results, null findings, and replication failures in Agricultural and Biological Sciences. Search the index →

WASTE indexes published research — it does not host or republish full papers. Each entry is a metadata record compiled from open scholarly databases; the abstract is shown in full only where the paper is openly licensed, otherwise a short excerpt under fair use. Classifications are automated and approximate.

Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

The nsp1, nsp13, and M Proteins Contribute to the Hepatotropism of Murine Coronavirus JHM.WU

Rong Zhang, Yize Li, Timothy J. Cowley et al. · 2015 · Journal of Virology

UNLABELLED: Mouse hepatitis virus (MHV) isolates JHM.WU and JHM.SD promote severe central nervous system disease. However, while JHM.WU replicates robustly and induces hepatitis, JHM.SD fails to replicate or induce pathology in the liver.…

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Phylogenomic resolution of order- and family-level monocot relationships using 602 single-copy nuclear genes and 1375 BUSCO genes

Prakash Raj Timilsena, Eric Wafula, Craig F. Barrett et al. · 2022 · Frontiers in Plant Science

We assess relationships among 192 species in all 12 monocot orders and 72 of 77 families, using 602 conserved single-copy (CSC) genes and 1375 benchmarking single-copy ortholog (BUSCO) genes extracted from genomic and transcriptomic datasets. Phylogenomic inferences based on these data, using both coalescent-based and supermatrix analyses, are largely congruent with the most comprehensive plastome-based analysis, and nuclear-gene phylogenomic analyses with less comprehensive taxon sampling. The strongest discordance between the plastome and nuclear gene analyses is the monophyly of a clade com

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Does Urban and Peri-Urban Agriculture Contribute to Household Food Security? An Assessment of the Food Security Status of Households in Tongaat, eThekwini Municipality

Nolwazi Z. Khumalo, Melusi Sibanda · 2019 · Sustainability

Regardless of the steady increase in the economic growth of South Africa, poverty levels and food insecurity have not declined as one would have anticipated. Urban and peri-urban agriculture (UPA) presents an opportunity as a livelihood strategy to alleviate poverty and ensure household food security within the urban and peri-urban spheres. However, less research has been done in this area to discover the contribution of UPA on households’ food security. This paper assesses the food security status of households that practised any form of UPA activities (later on referred to the rest of this p

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

In-depth proteomic analysis of Varroa destructor: Detection of DWV-complex, ABPV, VdMLV and honeybee proteins in the mite

Tomáš Erban, Karel Harant, Martin Hubálek et al. · 2015 · Scientific Reports

We investigated pathogens in the parasitic honeybee mite Varroa destructor using nanoLC-MS/MS (TripleTOF) and 2D-E-MS/MS proteomics approaches supplemented with affinity-chromatography to concentrate trace target proteins. Peptides were detected from the currently uncharacterized Varroa destructor Macula-like virus (VdMLV), the deformed wing virus (DWV)-complex and the acute bee paralysis virus (ABPV). Peptide alignments revealed detection of complete structural DWV-complex block VP2-VP1-VP3, VDV-1 helicase and single-amino-acid substitution A/K/Q in VP1, the ABPV structural block VP1-VP4-VP2-

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Negative / Null Result ReportAgricultural and Biological Sciences

Importance of the relative levels of dietary arachidonic acid and eicosapentaenoic acid for culture performance of gilthead seabream (Sparus aurata) larvae

Eyad Atalah, C.M Hernández-Cruz, T. Benítez-Santana et al. · 2010 · Aquaculture Research

The effect of different arachidonic acid (ARA) dietary contents at several dietary eicosapentaenoic acid (EPA) levels on the growth, survival and biochemical composition of gilthead seabream larvae was studied to better define the…

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Negative / Null Result ReportAgricultural and Biological Sciences

Zolpidem Reduces Postoperative Pain, Fatigue, and Narcotic Consumption Following Knee Arthroscopy: A Prospective Randomized Placebo-Controlled Double-Blinded Study

Robert Z. Tashjian, Rahul Banerjee, Michael P. Bradley et al. · 2010 · The Journal of Knee Surgery

Sixty-eight patients undergoing outpatient knee arthroscopy for treatment of meniscal tears or loose bodies were divided into three treatment groups (zolpidem [24 patients], control [24 patients], and placebo [20 patients]). All groups…

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Conventional and organic farms with more intensive management have lower soil functionality

Sophie Q. van Rijssel, Guusje J. Koorneef, G. F. Veen et al. · 2025 · Science

Organic farming is often considered to be more sustainable than conventional farming. However, both farming systems comprise highly variable management practices. In this study, we show that in organic and conventional arable fields, the…

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Science losing its way: examples from the realm of microbial N2-fixation in cereals and other non-legumes

K.E. Giller, Euan K. James, Julie Ardley et al. · 2024 · Plant and Soil

Abstract Background Despite more than 50 years of research, no robust evidence suggests that inoculation of cereals and other non-legumes with free-living and/or endophytic bacteria leads to fixation of agronomically significant quantities of dinitrogen gas (N 2 ) from the atmosphere. A plethora of new products claims to increase the growth and yields of major cereals and other crops through stimulating N 2 -fixation by inoculating with bacteria. Scope We review the literature on N 2 -fixation by bacteria in the rhizosphere and as endophytes in non-legume plants. We find no unequivocal evidenc

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Variation in Significant Difference of Sausage Textural Parameters Measured by Texture Profile Analysis (TPA) under Changing Measurement Conditions

Sun-Hwa Shin, Won-Seok Choi · 2021 · Food Science of Animal Resources

This study examined the measurement conditions of the texture profile analysis (TPA) experiments that are typically used to measure the physical properties of sausage. As the measurement conditions (compression ratio and cross-head speed)…

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

A viroid-derived system to produce large amounts of recombinant RNA in Escherichia coli

José‐Antonio Daròs, Verónica Aragonés, Teresa Cordero · 2018 · Scientific Reports

Viruses have been engineered into useful biotechnological tools for gene therapy or to induce the synthesis of products of interest, such as therapeutic proteins and vaccines, in animal and fungal cells, bacteria or plants. Viroids are a particular class of infectious agents of higher plants that exclusively consist of a small non-protein-coding circular RNA molecule. In the same way as viruses have been transformed into useful biotechnological devices, can viroids be converted into beneficial tools? We show herein that, by expressing Eggplant latent viroid (ELVd) derived RNAs in Escherichia c

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Negative / Null Result ReportAgricultural and Biological Sciences

Effects of within-generation thermal history on flight performance ofCeratitis capitata: colder is better

Nanike Esterhuizen, Susana Clusella‐Trullas, Corné E. van Daalen et al. · 2014 · Journal of Experimental Biology

The influence of thermal history on temperature-dependent flight performance was investigated in an invasive agricultural pest insect, Ceratitis capitata (Diptera: Tephritidae). Flies were exposed to one of four developmental acclimation…

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Negative / Null Result ReportAgricultural and Biological Sciences

How Do Predators Learn to Recognize a Mimetic Complex: Experiments with Naive Great Tits and Aposematic Heteroptera

Kateřina Hotová Svádová, Alice Exnerová, Michala Kopečková et al. · 2013 · Ethology

Abstract We tested the importance of innate wariness, avoidance learning, memory and generalization for the formation of predatory behaviour in naive great tits ( P arus major ) towards mimetic complex of four aposematic species of true…

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Intensifying cropping systems through doubled-up legumes in Eastern Zambia

Mulundu Mwila, Blessing Mhlanga, Christian Thierfelder · 2021 · Scientific Reports

Abstract Declining soil fertility and negative impacts of climate effects threaten the food security of millions in Africa. Conservation Agriculture (CA) is a promising strategy to address these challenges. However, lack of viable economic entry points and short-term benefits for smallholders limit its adoption. Legume intensification can possibly increase the output per unit area, thus making the system more attractive. Rotations of maize with intensified legume systems were tested for three consecutive years under ridge and furrow (RF) tillage and CA to investigate: (a) increases in producti

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Assessment of potential enhancing effects of a carbohydrase mixture on phytase efficacy in male broiler chicks fed phosphorus-deficient diets from 1 to 18 days of age

Ahmad Karimi, Yuna Min, C. Lu et al. · 2012 · Poultry Science

A total of 540 one-day-old male broiler chicks were used to evaluate the potential enhancing effects of a mixture of 2 commercial carbohydrase preparations on phytase efficacy. A nonphytate phosphorus (nPP) deficient diet (0.15%) was…

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Yeast Species, Strains, and Growth Media Mediate Attraction of Drosophila suzukii (Diptera: Drosophilidae)

Rodrigo Lasa, Laura Navarro-de-la-Fuente, Anne Gschaedler et al. · 2019 · Insects

Specific ecological interactions between insects and microbes have potential in the development of targeted pest monitoring or control techniques for the spotted wing drosophilid, Drosophila suzukii (Matsumura), an exotic invasive pest of soft fruit. To evaluate D. suzukii attraction to yeast species from preferred types of fruit, three yeasts were isolated from blackberry fruit and two yeasts from raspberry fruit and used to bait simple plastic bottle traps. Saccharomyces cerevisiae and Hanseniaspora uvarum were identified from blackberries, whereas a different H. uvarum strain was identified

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Unmanned Ground Vehicles for Continuous Crop Monitoring in Agriculture: Assessing the Readiness of Current ICT Technology

Maurizio Agelli, Nicola Corona, Fabio Maggio et al. · 2024 · Machines

Continuous crop monitoring enables the early detection of field emergencies such as pests, diseases, and nutritional deficits, allowing for less invasive interventions and yielding economic, environmental, and health benefits. The work organization of modern agriculture, however, is not compatible with continuous human monitoring. ICT can facilitate this process using autonomous Unmanned Ground Vehicles (UGVs) to navigate crops, detect issues, georeference them, and report to human experts in real time. This review evaluates the current state of ICT technology to determine if it supports auton

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Local-scale virome depiction in Medellín, Colombia, supports significant differences between Aedes aegypti and Aedes albopictus

Arley Calle-Tobón, Juliana Pérez-Pérez, Nicolás Forero-Pineda et al. · 2022 · PLoS ONE

Aedes spp. comprise the primary group of mosquitoes that transmit arboviruses such as dengue, Zika, and chikungunya viruses to humans, and thus these insects pose a significant burden on public health worldwide. Advancements in next-generation sequencing and metagenomics have expanded our knowledge on the richness of RNA viruses harbored by arthropods such as Ae. aegypti and Ae. albopictus. Increasing evidence suggests that vector competence can be modified by the microbiome (comprising both bacteriome and virome) of mosquitoes present in endemic zones. Using an RNA-seq-based metataxonomic app

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Comparative transcriptome analysis reveals significant differences in the regulation of gene expression between hydrogen cyanide- and ethylene-treated Arabidopsis thaliana

Lulu Yu, Yang Liu, Fei Xu · 2019 · BMC Plant Biology

BACKGROUND: Hydrogen cyanide (HCN) is a small gaseous molecule that is predominantly produced as an equimolar co-product of ethylene (ET) biosynthesis in plants. The function of ET is of great concern and is well studied; however, the function of HCN is largely unknown. Similar to ET, HCN is a simple and diffusible molecule that has been shown to play a regulatory role in the control of some metabolic processes in plants. Nevertheless, it is still controversial whether HCN should be regarded as a signalling molecule, and the cross-talk between HCN and ET in gene expression regulation remains u

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Identification of the Genome Segments of Bluetongue Virus Serotype 26 (Isolate KUW2010/02) that Restrict Replication in a Culicoides sonorensis Cell Line (KC Cells)

Gillian D. Pullinger, Marc Guimerà Busquets, Kyriaki Nomikou et al. · 2016 · PLoS ONE

Bluetongue virus (BTV) can infect most ruminant species and is usually transmitted by adult, vector-competent biting midges (Culicoides spp.). Infection with BTV can cause severe clinical signs and can be fatal, particularly in naïve sheep and some deer species. Although 24 distinct BTV serotypes were recognized for several decades, additional 'types' have recently been identified, including BTV-25 (from Switzerland), BTV-26 (from Kuwait) and BTV-27 from France (Corsica). Although BTV-25 has failed to grow in either insect or mammalian cell cultures, BTV-26 (isolate KUW2010/02), which can be t

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Cry1Ac Transgenic Sugarcane Does Not Affect the Diversity of Microbial Communities and Has No Significant Effect on Enzyme Activities in Rhizosphere Soil within One Crop Season

Dinggang Zhou, Liping Xu, Shiwu Gao et al. · 2016 · Frontiers in Plant Science

Cry1Ac transgenic sugarcane provides a promising way to control stem-borer pests. Biosafety assessment of soil ecosystem for cry1Ac transgenic sugarcane is urgently needed because of the important role of soil microorganisms in nutrient transformations and element cycling, however little is known. This study aimed to explore the potential impact of cry1Ac transgenic sugarcane on rhizosphere soil enzyme activities and microbial community diversity, and also to investigate whether the gene flow occurs through horizontal gene transfer. We found no horizontal gene flow from cry1Ac sugarcane to soi

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Introducing the No-Significant-Effect Concentration

Rebecca Fisher, David R. Fox · 2023 · Environmental Toxicology and Chemistry

The no-effect concentration (NEC) is the preferred threshold metric for single-species toxicity tests applied to derive safe concentration thresholds for contaminants in the environment for use in species sensitivity distributions.…

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Modified cyclodextrin type and dehydration methods exert a significant effect on the antimicrobial activity of encapsulated carvacrol and thymol

Santiago López‐Miranda, Daniel Berdejo, Elisa Pagán et al. · 2020 · Journal of the Science of Food and Agriculture

Abstract BACKGROUND The antimicrobial activity of essential oils and their constituents has led to increasing interest in using them as natural preservative agents. However, their high sensitivity to light and oxygen, their volatility and…

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Negative / Null Result ReportAgricultural and Biological Sciences

Forest reclamation of fly ash deposit: a field study on appraisal of mycorrhizal inoculation

Zuzana Sýkorová, Jana Rydlová, Renata Slavíková et al. · 2015 · Restoration Ecology

An extensive field trial was established on a fly ash deposit (1) to evaluate whether the inoculation with arbuscular mycorrhizal fungi ( AMF ) and/or ectomycorrhizal fungi ( EcMF ) improves growth and survival of 13 planted tree species…

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Technological characterization and survival of the exopolysaccharide-producing strain Lactobacillus delbrueckii subsp. lactis 193 and its bile-resistant derivative 193+ in simulated gastric and intestinal juices

Patricia Burns, Gabriel Vinderola, Jorge Reinheimer et al. · 2011 · Journal of Dairy Research

The capacity of lactic acid bacteria to produce exopolysaccharides (EPS) conferring microorganisms a ropy phenotype could be an interesting feature from a technological point of view. Progressive adaptation to bile salts might render some…

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Educated Millennials and Credence Attributes of Food Products with Genetically Modified Organisms: Knowledge, Trust and Social Media

Carlo Russo, Mariarosaria Simeone, Maria Angela Perito · 2020 · Sustainability

In this paper, we investigated educated millennials’ evaluation of credence attributes in food products containing genetically modified organisms (GMO products). Our goal is to assess whether beliefs about GMO products are determined by scientific knowledge alone or if they are affected by other factors such as trust in information providers and use of social media. The focus on millennials is motivated by the increasing relevance of this social group in the public debate and by their extensive use of social media. We surveyed a sample of 215 Italian college students, confronting them with que

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Negative / Null Result ReportAgricultural and Biological Sciences

Significant effect of NSP‐ase enzyme supplementation in sunflower meal‐based diet on the growth and nutrient digestibility in broilers

Muhammad Bilal, Muhammad Aslam Mirza, Muhammad Kaleem et al. · 2016 · Journal of Animal Physiology and Animal Nutrition

The response of broiler chickens to 3 levels of sunflower meal and 2 levels of NSP-ase enzyme combination (with and without) was investigated in 3 × 2 factorial arrangement under complete randomized design (CRD). A total of 240 Hubbard…

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Intercropping changed the soil microbial community composition but no significant effect on alpha diversity

Jiaying Liu, Weixi Zhang, Chao Teng et al. · 2024 · Frontiers in Microbiology

Introduction: Enhancing the planning of the forest-agricultural composite model and increasing the efficiency with which forest land is utilized could benefit from a thorough understanding of the impacts of intercropping between forests and agriculture on soil physicochemical properties and microbial communities. Methods: intercrop soils, along with their corresponding monocrops, were used in this study's llumina high-throughput sequencing analysis to determine the composition and diversity of soil bacterial and fungal communities. Results: The findings indicated that intercropping considerabl

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Refining Pheromone Lures for the Invasive Halyomorpha halys (Hemiptera: Pentatomidae) Through Collaborative Trials in the United States and Europe

Tracy Leskey, Heather Andrews, Angelika Bády et al. · 2021 · Journal of Economic Entomology

Brown marmorated stink bug, Halyomorpha halys, is native to Asia and has invaded North America and Europe inflicting serious agricultural damage to specialty and row crops. Tools to monitor the spread of H. halys include traps baited with…

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