Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Rebecca C. Richmond, Carlos Sillero‐Rejon, Jasmine Khouja et al. · 2021 · Clinical Epigenetics
Abstract Background Little evidence exists on the health effects of e-cigarette use. DNA methylation may serve as a biomarker for exposure and could be predictive of future health risk. We aimed to investigate the DNA methylation profile of e-cigarette use. Results Among 117 smokers, 117 non-smokers and 116 non-smoking vapers, we evaluated associations between e-cigarette use and epigenome-wide methylation from saliva. DNA methylation at 7 cytosine-phosphate-guanine sites (CpGs) was associated with e-cigarette use at p < 1 × 10 –5 and none at p < 5.91 × 10 –8 . 13 CpGs were associated wi
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
I. Krzyzewska, Judith Ensink, Laura Nawijn et al. · 2018 · European Journal of Human Genetics
Posttraumatic stress disorder (PTSD) is a debilitating psychiatric disorder that may develop after a traumatic event. Here we aimed to identify epigenetic and genetic loci associated with PTSD. We included 73 traumatized police officers…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Mark P. Purdue, Yuanqing Ye, Zhaoming Wang et al. · 2013 · Cancer Epidemiology Biomarkers & Prevention
Genome-wide association studies (GWAS) of renal cell carcinoma (RCC) in populations of European ancestry have identified four susceptibility loci. No GWAS has been conducted among African Americans (AA), who experience a higher incidence…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Xiaona Yang, Hong Guo, Min Zou · 2026 · Molecular Biomedicine
Inflammatory bowel disease (IBD) is a heterogeneous group of disorders characterized primarily by chronic relapsing intestinal inflammation, encompassing Crohn's disease (CD) and ulcerative colitis (UC), affecting individuals across age groups with variable clinical manifestations. With the advancement of global industrialization, its incidence continues to rise, particularly in newly industrialized regions, which not only severely impairs patients' quality of life but also emerges as a major public health concern threatening digestive system health, accompanied by a substantial healthcare bur
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
W-J Xiao, J-W He, H Zhang et al. · 2010 · International Journal of Obesity
OBJECTIVE: Arachidonate 12-lipoxygenase (ALOX12) is a member of the lipoxygenase superfamily, which catalyzes the incorporation of molecular oxygen into polyunsaturated fatty acids. The products of ALOX12 reactions serve as endogenous…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Sean M. Carroll, Katherine S. Xue, Christopher J. Marx · 2014 · BMC Microbiology
BACKGROUND: A common assumption of microorganisms is that laboratory stocks will remain genetically and phenotypically constant over time, and across laboratories. It is becoming increasingly clear, however, that mutations can ruin strain integrity and drive the divergence or "domestication" of stocks. Since its discovery in 1960, a stock of Methylobacterium extorquens AM1 ("AM1") has remained in the lab, propagated across numerous growth and storage conditions, researchers, and facilities. To explore the extent to which this lineage has diverged, we compared our own "Modern" stock of AM1 to a
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Danli Xiao, Huarong Zhang, Ye Liu et al. · 2026 · Signal Transduction and Targeted Therapy
Oncolytic viruses (OVs) represent a promising strategy in cancer immunotherapy, as they selectively infect and lyse tumor cells while simultaneously triggering robust antitumor immune responses. By inducing immunogenic cell death, OVs enhance tumor antigen presentation and initiate a systemic immune response, effectively transforming the tumor microenvironment from an immune-suppressive state to an immune-permissive state. In addition to exerting direct oncolytic effects, OVs modulate key tumor-associated biological processes, including tumor angiogenesis and extracellular matrix remodeling, d
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Oriah Mioduser, Eli Goz, Tamir Tuller · 2017 · BMC Genomics
BACKGROUND: Viruses undergo extensive evolutionary selection for efficient replication which effects, among others, their codon distribution. In the current study, we aimed at understanding the way evolution shapes the codon distribution in early vs. late viral genes in terms of their expression during different stages in the viral replication cycle. To this end we analyzed 14 bacteriophages and 11 human viruses with available information about the expression phases of their genes. RESULTS: We demonstrated evidence of selection for distinct composition of synonymous codons in early and late vi
View details →Failed Experiment ReportOpen accessBiochemistry, Genetics and Molecular Biology
Kevin G. Shim, Shane Zaidi, Jill Thompson et al. · 2017 · Molecular Therapy
Systemic viroimmunotherapy activates endogenous innate and adaptive immune responses against both viral and tumor antigens. We have shown that therapy with vesicular stomatitis virus (VSV) engineered to express a tumor-associated antigen…
View details →Negative / Null Result ReportBiochemistry, Genetics and Molecular Biology
Ying Shen, Yuanlong Yan, Yunqiang Liu et al. · 2013 · Human Molecular Genetics
AZFc deletions cause a significant phenotypic heterogeneity with respect to spermatogenesis; however, the reason for this is poorly understood. Recently, testis-specific protein Y-encoded 1 (TSPY1) copy number variation (CNV) was…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Masaru Sekijima, Hiroshi Takeda, Katsuaki Yasunaga et al. · 2010 · Journal of Radiation Research
We investigated the mechanisms by which radiofrequency (RF) fields exert their activity, and the changes in both cell proliferation and the gene expression profile in the human cell lines, A172 (glioblastoma), H4 (neuroglioma), and IMR-90…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Eamonn P. Culligan, Julian R. Marchesi, Colin Hill et al. · 2014 · Frontiers in Microbiology
In the current study, a number of salt-tolerant clones previously isolated from a human gut metagenomic library were screened using Phenotype MicroArray (PM) technology to assess their functional capacity. PM's can be used to study gene function, pathogenicity, metabolic capacity and identify drug targets using a series of specialized microtitre plate assays, where each well of the microtitre plate contains a different set of conditions and tests a different phenotype. Cellular respiration is monitored colorimetrically by the reduction of a tetrazolium dye. One clone, SMG 9, was found to be po
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Boshu Ru, Dingcheng Li, Yueqi Hu et al. · 2019 · IEEE Transactions on NanoBioscience
Serendipitous drug usage refers to the unexpected relief of comorbid diseases or symptoms when taking medication for a different known indication. Historically, serendipity has contributed significantly to identifying many new drug…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Heini Sánez Tähtisalo, Sanni Ruotsalainen, Nina Mars et al. · 2020 · Scientific Reports
Polygenic risk scores (PRSs) for essential hypertension, calculated from > 900 genomic loci, were recently found to explain a significant fraction of hypertension heritability and complications. To investigate whether variation of hypertension PRS also captures variation of antihypertensive drug responsiveness, we calculated two different PRSs for both systolic and diastolic blood pressure: one based on the top 793 independent hypertension-associated single nucleotide polymorphisms and another based on over 1 million genome-wide variants. Using our pharmacogenomic GENRES study comprising four
View details →Negative / Null Result ReportBiochemistry, Genetics and Molecular Biology
Bo Xi, Yue Shen, Kathleen H. Reilly et al. · 2012 · Clinical Endocrinology
OBJECTIVE: Recent genome-wide association studies have identified a few single nucleotide polymorphisms (SNPs), which are associated with body mass index (BMI)/obesity. This study aimed to examine the identified associations among a…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Song Wang, Cecilie L. Bager, M.A. Karsdal et al. · 2021 · Journal of Translational Medicine
BACKGROUND: Extensive extracellular matrix (ECM) remodeling is a hallmark of metastatic pancreatic ductal adenocarcinoma (mPDA). We investigated fragments of collagen types III (C3M, PRO-C3), VI (PRO-C6), and VIII (C8-C), and versican (VCANM) in plasma as biomarkers for predicting progression-free survival (PFS) and overall survival (OS) in patients with mPDA treated with pegvorhyaluronidase alfa, a biologic that degrades the ECM component hyaluronan (HA), in a randomized phase 2 study (HALO109-202). METHODS: HALO109-202 comprised a discovery cohort (Stage 1, n = 94) and a validation cohort (S
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Anarkali Mahmood, Zdeněk Otruba, Alan W. Weisgerber et al. · 2023 · Biophysical Journal
When multivesicular endosomes (MVEs) fuse with the plasma membrane, exosomes are released into the extracellular space where they can affect other cells. The ability of exosomes to regulate cells nearby or further away depends on whether…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Juan Zeng, Yukun Li, Feifei Quan et al. · 2020 · Molecular Medicine Reports
Propofol, a commonly used intravenous anesthetic agent during surgery, has relatively widespread pharmacological actions. Previous studies have reported that propofol may act as an antitumor drug in several cancer types, such as pancreatic…
View details →Replication FailureOpen accessBiochemistry, Genetics and Molecular Biology
Serena Porcari, Chiara CICCARESE, Vitor Heidrich et al. · 2026 · Nature Medicine
Renal cell carcinoma (RCC) is a common malignancy with limited durable responses to first-line immune checkpoint inhibitor (ICI)-based therapies. Emerging evidence implicates the gut microbiome in modulating ICI efficacy. In the…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Araiz Hussain · 2025 · Cardiovascular Diabetology – Endocrinology Reports
Chronic hyperglycemia is the defining feature of type 2 diabetes mellitus (T2DM) and a central driver of its long-term complications, including microvascular and macrovascular diseases. Among these, cardiovascular disease (CVD) remains the leading cause of morbidity and mortality in individuals with T2DM. Persistent glucose elevation activates multiple interrelated biochemical pathways, including the polyol pathway, formation of advanced glycation end-products, protein kinase C activation, oxidative stress via several cellular mechanisms, as well as the hexosamine biosynthetic pathway. These p
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Anna L. Guyatt, Kimberley Burrows, Philip A. I. Guthrie et al. · 2017 · Mitochondrion
The mitochondrial genome is present at variable copy number between individuals. Mitochondria are vulnerable to oxidative stress, and their dysfunction may be associated with cardiovascular disease. The association of mitochondrial DNA copy number with cardiometabolic risk factors (lipids, glycaemic traits, inflammatory markers, anthropometry and blood pressure) was assessed in two independent cohorts of European origin women, one in whom outcomes were measured at mean (SD) age 30 (4.3) years (N=2278) and the second at 69.4 (5.5) years (N=2872). Mitochondrial DNA copy number was assayed by qua
View details →Negative / Null Result ReportBiochemistry, Genetics and Molecular Biology
Bart de Boer · 2010 · Journal of Evolutionary Psychology
This paper investigates the effect of larynx position on the articulatory abilities of a human-like vocal tract. Previous work has investigated models that were built to resemble the anatomy of existing species or fossil ancestors. This…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Jorma J. de Ronde, Christiaan Klijn, Arno Velds et al. · 2010 · BMC Research Notes
BACKGROUND: Most approaches used to find recurrent or differential DNA Copy Number Alterations (CNA) in array Comparative Genomic Hybridization (aCGH) data from groups of tumour samples depend on the discretization of the aCGH data to gain, loss or no-change states. This causes loss of valuable biological information in tumour samples, which are frequently heterogeneous. We have previously developed an algorithm, KC-SMART, that bases its estimate of the magnitude of the CNA at a given genomic location on kernel convolution (Klijn et al., 2008). This accounts for the intensity of the probe sign
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Mark O. Goodarzi, Nicholette D Palmer, Jinrui Cui et al. · 2019 · The Journal of Clinical Endocrinology & Metabolism
CONTEXT: Genome-wide association studies have identified more than 450 single nucleotide polymorphisms (SNPs) for type 2 diabetes (T2D). OBJECTIVE: To facilitate use of these SNPs in future genetic risk score (GRS)-based analyses, we aimed…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Sergio Garnica-Galvez, Stefanie Korntner, Ioannis Skoufos et al. · 2021 · Cells
The use of macromolecular crowding in the development of extracellular matrix-rich cell-assembled tissue equivalents is continuously gaining pace in regenerative engineering. Despite the significant advancements in the field, the optimal macromolecular crowder still remains elusive. Herein, the physicochemical properties of different concentrations of different molecular weights hyaluronic acid (HA) and their influence on equine adipose-derived stem cell cultures were assessed. Within the different concentrations and molecular weight HAs, the 10 mg/mL 100 kDa and 500 kDa HAs exhibited the high
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Carmella Evans‐Molina, Richard A. Oram · 2025 · Diabetes Obesity and Metabolism
Type 1 diabetes (T1D) has been historically regarded as a childhood-onset disease; however, recent epidemiological data indicate that adult-onset T1D accounts for a substantial proportion of cases worldwide. There is evidence that…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Matthijs J. H. M. van der Loos, Philipp Koellinger, Patrick J. F. Groenen et al. · 2011 · Small Business Economics
Candidate gene studies of human behavior are gaining interest in economics and entrepreneurship research. Performing and interpreting these studies is not straightforward because the selection of candidates influences the interpretation of…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Neil D. Clarke, James R. Thomas, Marion Kagka et al. · 2016 · The Journal of Strength and Conditioning Research
Clarke, ND, Thomas, JR, Kagka, M, Ramsbottom, R, and Delextrat, A. No dose-response effect of carbohydrate mouth rinse concentration on 5-km running performance in recreational athletes. J Strength Cond Res 31(3): 715-720, 2017-Oral…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Benjamin L. Rambo‐Martin, Jennifer G. Mulle, David J. Cutler et al. · 2017 · G3 Genes Genomes Genetics
One in five people with Down syndrome (DS) are born with an atrioventricular septal defect (AVSD), an incidence 2000 times higher than in the euploid population. The genetic loci that contribute to this risk are poorly understood. In this study, we tested two hypotheses: (1) individuals with DS carrying chromosome 21 copy number variants (CNVs) that interrupt exons may be protected from AVSD, because these CNVs return AVSD susceptibility loci back to disomy, and (2) individuals with DS carrying chromosome 21 genes spanned by microduplications are at greater risk for AVSD because these microdup
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Martin Boegemann, Katrin Schlack, Ann-Kathrin Fischer et al. · 2016 · PLoS ONE
OBJECTIVE: Even though the exact mechanism is largely unknown until now, statins are supposed to improve survival outcomes in various malignancies. For prostate cancer however, statins are known to compete with dehydroepiandrosterone (DHEAS) for the transport into the cytosol both using the cell by the Solute Carrier Transporter and thus diminish the cellular uptake of DHEAS as a precursor of androgens. Abiraterone inhibits CYP17A1 and thus effectively decreases the production of all relevant androgens including DHEAS. In this study we examined whether statins still affect survival outcome in
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