Laboratory divergence of Methylobacterium extorquens AM1 through unintended domestication and past selection for antibiotic resistance
Sean M. Carroll; Katherine S. Xue; Christopher J. Marx · 2014 · BMC Microbiology
WASTE classifies this as Negative / Null Result Report · AI classification, approximate
The study found no significant effect — useful as a negative control or null benchmark for your own design.
Abstract
BACKGROUND: A common assumption of microorganisms is that laboratory stocks will remain genetically and phenotypically constant over time, and across laboratories. It is becoming increasingly clear, however, that mutations can ruin strain integrity and drive the divergence or "domestication" of stocks. Since its discovery in 1960, a stock of Methylobacterium extorquens AM1 ("AM1") has remained in the lab, propagated across numerous growth and storage conditions, researchers, and facilities. To explore the extent to which this lineage has diverged, we compared our own "Modern" stock of AM1 to a
Abstract by Sean M. Carroll; Katherine S. Xue; Christopher J. Marx, BMC Microbiology (2014) — licensed CC BY 4.0.
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Metadata source: OpenAlex · DOI 10.1186/1471-2180-14-2