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Browse the failure-mode index

12,246 real negative results, null findings, and replication failures in Medicine. Search the index →

WASTE indexes published research — it does not host or republish full papers. Each entry is a metadata record compiled from open scholarly databases; the abstract is shown in full only where the paper is openly licensed, otherwise a short excerpt under fair use. Classifications are automated and approximate.

Negative / Null Result ReportOpen accessMedicine

The application value and limitations of metagenomic detection technology based on cerebrospinal fluid samples in suspected central nervous system infection: a retrospective study.

Feng Q, Liu B, Liu H et al. · 2025 · Frontiers in microbiology

Background Accurately diagnosing central nervous system (CNS) infections remains challenging. This study aimed to evaluate the effectiveness of metagenomic next-generation sequencing (mNGS) in diagnosing suspected CNS infections and its…

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Failed Experiment ReportOpen accessMedicine

Atypical superotemporal iris and retinal coloboma.

Patterson KN, Reynolds MM, Lueder GT · 2026 · American journal of ophthalmology case reports

Purpose To report our findings in a patient with an atypical case of supertemporal iris and retinal coloboma, and to assess its systemic associations and potential genetic causes. Observations A premature female infant born at 31 weeks…

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Negative / Null Result ReportOpen accessMedicine

Smear-positive Pulmonary Tuberculosis with False-negative Loop-mediated Isothermal Amplification and T-SPOT.TB Assays.

Tsutsumi Y, Nagasaki T, Muraki M et al. · 2026 · Internal medicine (Tokyo, Japan)

Rapid molecular and immunological assays for tuberculosis, including loop-mediated isothermal amplification (LAMP) and T-SPOT.TB assays, may yield false-negative results. We herein report a 62-year-old immunocompetent man with a productive…

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Negative / Null Result ReportOpen accessMedicine

Analytical performance of species-targeted quantitative PCR for intra-abdominal candidiasis in critically ill patients: a proof-of-concept post hoc analysis from the pBDG2 multicenter study.

Novy E, Schaeffer A, Nguyen M et al. · 2026 · Critical care (London, England)

BACKGROUND: Intra-abdominal candidiasis (IAC) is a severe form of invasive candidiasis that affects critically ill patients and is associated with a high mortality rate and persistent diagnostic delays. Conventional fungal culture from…

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Negative / Null Result ReportMedicine

A study of patient recall and comprehension of genetic testing results in amyotrophic lateral sclerosis (ALS).

Tomlinson J, Roberson E, Klee V et al. · 2026 · Amyotrophic lateral sclerosis & frontotemporal degeneration

Objective: Assess the accuracy of ALS patient recall of genetic testing results and evaluate comprehension of key implications of results. Methods: Participants were recruited from the Center for Disease Control's National ALS Registry. A…

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Negative / Null Result ReportMedicine

Metagenomic Next-generation Sequencing of Cerebrospinal Fluid: First-year Experience at a Tertiary Referral Hospital.

Alvarez Mulett S, Molina Garcia S, Abu Saleh O et al. · 2026 · Clinical infectious diseases : an official publication of the Infectious Diseases Society of America

Background Central nervous system (CNS) infections are significant causes of morbidity and mortality, especially when diagnosis is delayed. Traditional cerebrospinal fluid diagnostic methods for CNS infections have limited sensitivity.…

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Negative / Null Result ReportOpen accessMedicine

Diagnostic performance and clinical limitations of sentinel lymph node biopsy in head and neck melanoma: a 20-year retrospective cohort study.

Lükewille L, Wiegand S, Fazel A et al. · 2026 · Journal of cancer research and clinical oncology

Purpose Sentinel lymph node biopsy (SLNB) is a standard procedure for nodal staging in cutaneous melanoma. However, its diagnostic performance in the head and neck region remains limited due to complex lymphatic drainage patterns. This…

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Negative / Null Result ReportMedicine

[Analysis of false-negative cases by Optical genome mapping and a literature review].

Zhang J, Su M, Zhang Y et al. · 2025 · Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics

Objective To explore the reasons for false negative results by Optical genome mapping (OGM) analysis of three cases and propose strategies for handling them. Methods Three patients presented at the Affiliated Hospital of Nantong University…

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Negative / Null Result ReportMedicine

Cross-resistance of Belinostat and Romidepsin in Non-T Follicular Helper Peripheral T-cell Lymphoma Models Suggests Subtype-Specific Implications for Belinostat-CHOP.

Pantazis JC, Palmer AC · 2026 · Molecular cancer therapeutics

The phase III study of romidepsin plus CHOP for peripheral T-cell lymphoma (PTCL) yielded negative results in the overall analysis and showed that outcomes were only improved in the T follicular helper (TFH) subgroup of PTCL, with no…

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Negative / Null Result ReportOpen accessMedicine

Interpreting the drop: concordance and disparity between a new point-of-care assay and laboratory intraoperative parathyroid hormone testing in parathyroid surgery.

Kwatra D, Wakeford W, Ally M et al. · 2026 · BMC endocrine disorders

BACKGROUND: To evaluate the concordance of the NBCL CONNECT Point-of-Care (POC) Intraoperative Parathyroid Hormone (IOPTH) assay with a Central Laboratory standard, with a focus on percentage drops and the usefulness of POC testing in…

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Negative / Null Result ReportMedicine

Colostrum-derived immunity reduces detection of calves persistently infected with bovine viral diarrhea virus by common diagnostic tests.

Saucedo M, Chamorro MF, Passler T et al. · 2026 · Journal of the American Veterinary Medical Association

Objective This study aimed to compare the most accurate sample type, test, and timing for detecting bovine viral diarrhea virus (BVDV) in persistently infected (PI) calves at different time points following birth and colostrum intake.…

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Negative / Null Result ReportOpen accessMedicine

Disseminated Histoplasmosis and Aortic Valve Infective Endocarditis in a Patient With Recent Aortobifemoral Bypass.

Behjatnia H, Couto P, Castillo Y et al. · 2026 · The American journal of case reports

BACKGROUND Fungal endocarditis constitutes up to 3% of diagnosed infective endocarditis cases, and Histoplasma spp. cause approximately 10% of fungal endocarditis cases. Histoplasma infection occurs through inhalation of spores typically…

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Negative / Null Result ReportOpen accessMedicine

Concurrent <i>EGFR</i> L858R and K860I mutations: PCR-negative but NGS-positive alterations that respond to <i>EGFR</i> inhibitors in non-small cell lung cancer.

Shi H, Li W, Guo L et al. · 2025 · Journal of thoracic disease

Background Both amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) and next-generation sequencing (NGS) are able to identify sensitizing epidermal growth factor receptor ( EGFR ) mutations of non-small cell lung…

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Negative / Null Result ReportMedicine

Pegbelfermin in Patients With Nonalcoholic Steatohepatitis and Stage 3 Fibrosis (FALCON 1): A Randomized Phase 2b Study.

Loomba R, Sanyal AJ, Nakajima A et al. · 2024 · Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association

Background & aims Pegbelfermin is a polyethlene glycol-conjugated analog of human fibroblast growth factor 21, a nonmitogenic hormone that regulates energy metabolism. This phase 2b study evaluated 48-week pegbelfermin treatment in…

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Null DatasetOpen accessMedicine

The unsung hero: <i>ntnh</i> gene as complementary botulism marker.

Valdezate S, Valiente M, Díaz-Ramón S et al. · 2026 · Frontiers in cellular and infection microbiology

Botulism is a rare but severe neurological disease caused by botulinum neurotoxins (BoNTs). Standard diagnostic methods including the mouse bioassay (SMB) and bont gene type-specific PCR, are often limited by the high genetic diversity…

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Null DatasetOpen accessMedicine

Diagnostic performance of the Sepsis qPCR MX-30<sup>®</sup> panel compared with conventional blood culture in pediatric febrile neutropenia: a single-center retrospective study.

Kurtipek FB, Yozgat AK, Gökçek E et al. · 2026 · BMC infectious diseases

Background Febrile neutropenia (FN) is a frequent and potentially life-threatening complication in patients with hematologic malignancies. Prompt and accurate identification of bloodstream pathogens is essential to optimize antimicrobial…

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Replication FailureMedicine

The Limited Utility of Synovial C-Reactive Protein in the Diagnosis of Knee and Hip Periprosthetic Joint Infection: Proposed New Diagnostic Cutoffs.

McCavitt DG, Culler MW, Palmer RC et al. · 2026 · The Journal of arthroplasty

Background The utility of synovial C-reactive protein (CRP) in diagnosing periprosthetic joint infection (PJI) remains controversial. This study examined the performance of synovial CRP for PJI diagnosis following total knee arthroplasty…

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Replication FailureMedicine

Case report: false-negative Xpert MRSA/SA BC result due to spa gene deletion in a patient with methicillin-resistant Staphylococcus aureus bacteremia.

Mura T, Aso M, Kawamura K et al. · 2025 · Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy

Rapid diagnostic tests like the Xpert MRSA/SA BC assay are valuable for the early detection of Staphylococcus aureus (SA) and methicillin-resistant S. aureus (MRSA) in positive blood cultures. However, genetic variations in target regions…

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Negative / Null Result ReportMedicine

Nosocomial tuberculosis exposure events in the era of nucleic acid amplification testing: A root cause analysis.

Yang YJ, Lee MR, Chung CL et al. · 2026 · International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases

Objectives Despite the availability of rapid tuberculosis (TB) nucleic acid amplification (NAA) testing, prevention of TB transmission within healthcare facilities remains a challenge due to ongoing difficulties in promptly isolating…

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Failed Experiment ReportMedicine

Melatonin as a Supportive Biomarker in Early-Onset Neonatal Sepsis: A Prospective Study.

Kaya B, Can E, Karakurt Y et al. · 2026 · Advances in neonatal care : official journal of the National Association of Neonatal Nurses

Background Early-onset neonatal sepsis (EOS) remains a diagnostic challenge because clinical signs are nonspecific and blood cultures, although the diagnostic gold standard may yield false-negative results due to low blood volumes or…

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