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Failure-mode index

Search what already failed

A searchable index of real negative results, null findings, and replication failures from the published literature — so you can learn what didn't work before repeating it.

WASTE indexes published research — it does not host or republish full papers. Each entry is a metadata record (title, authors, DOI) compiled from open scholarly databases, with the abstract shown in full only where the paper is openly licensed (e.g. Creative Commons); otherwise a short excerpt is shown for reference under fair use. WASTE classifies each work by failure type; classifications are automated and approximate.

21350 results · page 566 of 712

Negative / Null Result ReportOpen accessMedicine

Origins of the obturator artery in Thai and Chinese

Damkoeng Purnindu · 2023 · Siriraj Medical Journal

Dissection of one-half of adult pelvis in 197 Thai and 44 Chinese cadavers disclosed that the obturator artery arose from the internal iliac and umbilical arteries in 34.9%, from the inferior gluteal artery in 10.4%, from the internal pudendal artery in 8.7%, from the superior gluteal - inferior gluteal trunk in 4.2%, from the inferior gluteal - internal pudendal trunk in 9.1%, from the inferior epigastric artery in 12.0%, from the external iliac artery in 1.2%, and from the superior gluteal and the inferior epigastric arteries in 0.4%. There were no statistically significant differences of fi

Negative / Null Result ReportOpen accessAnesthesiology

Comparison of malnutrition screening tests in predicting postoperative complications in elderly patients with femur fracture

Orhan Kılıç, Murat Yaşar Özkalkanlı, Fulya Yılmaz et al. · 2022 · Ain Shams Journal of Anesthesiology

Abstract Background Malnutrition is an important and widespread problem in hospitalised elderly orthopaedic patients. It is an important predictor of morbidity and mortality and has been associated with an increased risk of complications. Although it is a common problem in the elderly, there is neither a universally accepted criterion for the diagnosis of malnutrition nor a screening tool accepted as the gold standard to detect patients at risk of malnutrition. There is still no consensus on which is the most recommended for screening hospitalised patients for nutritional risk. The aim of this

View details →DOI: 10.1186/s42077-022-00280-9
Negative / Null Result ReportOpen accessDentistry

Correlation of CD10 immunoexpression and eosinophil count in stromal tissue as predictors of prognosis of squamous cell carcinoma of the oral cavity

Deece Patuti, Harmas Yazid Yusuf, Bethy Suryawathy Hernowo · 2010 · Padjadjaran Journal of Dentistry

Introduction: CD10 can also facilitate the ability of tumour metastasis by its ability to invade blood vessel walls.Correlation of CD10 immunoexpression and eosinophil count in stromal tissue as predictors of prognosis of squamous cell carcinoma of the oral cavity. Methods: This study was preceded by collecting data on squamous cell carcinoma of the oral cavity in the medical record and paraffin blocks in the Anatomy Pathology section of 15 samples with enlarged regional lymph nodes and 15 samples without enlargement of regional lymph nodes. Then two preparations were made, the first preparati

View details →DOI: 10.24198/pjd.vol22no2.26873
Negative / Null Result ReportOpen accessMedicine

Correlation of body mass index and orthostatic hypotension in patients with hypertension on ACE inhibitor monotherapy

Tasić Danijela, Kovačević Zorana, Mitrović Miroslav et al. · 2021 · Scripta Medica

Background/Aim: Orthostatic hypotension (OH) is considered to be a drop in the systolic and diastolic blood pressure (> 20 mmHg; > 10 mmHg) 3 minutes from postural changes. The objective of this study was to analyse the correlation of body mass index (BMI) and OH during the treatment with trandolapril, as a single-drug treatment of hypertension. Methods: The study involved 255 patients (average age 54.3 ± 11.7; 54.1 % men) with poorly regulated hypertension, who were given trandolapril as a single-drug treatment. The patients were divided into two groups regarding stage of hypertension: first-

View details →DOI: 10.5937/scriptamed52-32561
Negative / Null Result ReportOpen accessMedicine

Impact of an Online Gastrointestinal Symptom History Taker on Physician Documentation and Charting Time: Pragmatic Controlled Trial

Hall, Natalie J, Berry, Sameer K, Aguilar, Jack et al. · 2021 · JMIR Formative Research

BackgroundA potential benefit of electronic health records (EHRs) is that they could potentially save clinician time and improve documentation by auto-generating the history of present illness (HPI) in partnership with patients prior to the clinic visit. We developed an online patient portal called AEGIS (Automated Evaluation of Gastrointestinal [GI] Symptoms) that systematically collects patient GI symptom information and then transforms the data into a narrative HPI that is available for physicians to review in the EHR prior to seeing the patient. ObjectiveThis study aimed to compare whether

View details →DOI: 10.2196/23599
Negative / Null Result ReportOpen accessDiseases of the genitourinary system. Urology

Which localization method is optimal in ESWL: fluoroscopy or ultrasonography?

Dursun Baba, Necati Ekici, Arda Taşkın Taşkıran et al. · 2025 · BMC Urology

Abstract Background Urinary stone disease is a common urological disorder, particularly among middle-aged individuals. Extracorporeal Shock Wave Lithotripsy (ESWL) is often the first-line treatment for kidney and ureteral stones. Traditionally, fluoroscopy is used for stone targeting in ESWL, but it exposes patients and clinicians to radiation and cannot visualize non-opaque stones. Ultrasonographic targeting eliminates these issues. This study compares the advantages and disadvantages of fluoroscopy and ultrasound-targeted ESWL. Methods At Düzce University Hospital, 100 patients with radio-op

View details →DOI: 10.1186/s12894-025-01716-8
Negative / Null Result ReportOpen accessMicrobiology

Seroprevalence of Mycoplasma genitalium among Fertile and Infertile Women at Murtala Muhammad Specialist Hospital, Kano

Bakori, H. S., Kumurya, A. S., Idris, A. M. · 2024 · UMYU Journal of Microbiology Research

Mycoplasma genitalium is an emerging sexually transmitted pathogen with potential implications for reproductive health. This study aimed to investigate and compare the seroprevalence of M. genitalium among fertile and infertile women to provide insights into its potential association with fertility outcomes. A cross-sectional study was conducted involving a total of 90 women, including 31 fertile and 59 infertile women. Serum samples were collected and tested for the presence of M. genitalium-IgG antibodies using ELISA detection techniques. Data on demographic characteristics, including age an

View details →DOI: 10.47430/ujmr.2492.014
Negative / Null Result ReportOpen accessSurgery

Our midterm restenosis results using patch angioplasty closure versus primary closure in patients undergoing carotid endarterectomy: A comparative study

Taner İyigun, Mugisha Kyaruzi, Barış Timur et al. · 2019 · Turkish Journal of Vascular Surgery

Objectives: This study aims to compare midterm results of patch angioplasty with primary closure techniques in patients undergoing carotid endarterectomy (CEA) in terms of restenosis. Patients and methods: This prospective, single-center, randomized study included a total of 137 patients (42 females, 95 males; mean age 64.4±8.8 years; range 36 to 86 years) who underwent CEA between January 2010 and October 2015. The patients were divided into two groups: the patch angioplasty group (n=70) and the primary closure group (n=67). The mean follow-up was 2-7 years. Carotid computed tomography angiog

Negative / Null Result ReportOpen accessMedical technology

Effects of timing parameter changes on the gait of functional electrical stimulation users with drop foot

Simon Marchant, Shona Michael, Laura Milner et al. · 2019 · Journal of Rehabilitation and Assistive Technologies Engineering

Introduction Functional electrical stimulation uses clinician-set parameters to modify stimulation. This study aimed to investigate whether timing parameters in the ODFS Pace functional electrical stimulation device have an effect on the gait of the general population of functional electrical stimulation users who have a foot drop. Methods Twelve functional electrical stimulation users with foot drop resulting from upper motor neurone disorders were recruited from the functional electrical stimulation Service in Leeds, UK. A crossover trial design was used, comparing adjusted values of rising

View details →DOI: 10.1177/2055668319859142
Negative / Null Result ReportOpen accessEducation

نمط التعلم المقلوب (تدريس الأقران/ الاستقصائي) وأثر تفاعله مع استراتيجية التعلم المنظم ذاتيا (البحث عن المساعدة/ البحث عن المعلومات) على تنمية التحصيل الفوري والمرجأ ودافعية الإنجاز والرضا لدى طلاب تکنولوجيا التعليم

ولاء أحمد عباس مرسى · 2018 · تکنولوجیا التعلیم: سلسلة دراسات و بحوث

هدف البحث إلى تحديد انسب نمط لبيئة التعلم المقلوب (تدريس الأقران مقابل الاستقصائي) في إطار تفاعله مع استراتيجية التنظيم الذاتي للتعلم (البحث عن المساعدة مقابل البحث عن المعلومات)، ودراسة مدى تأثيرها في تنمية التحصيل الفوري والمرجأ، ودافعية الإنجاز، والرضا عن بيئة التعلم لدى طلاب تکنولوجيا التعليم. وقد استخدم في هذا البحث التصميم التجريبي ذي المجموعات التجريبية الأربعة، وهو امتداد للتصميم التجريبي ذي المجموعة الواحدة، واشتمل البحث على متغير مستقل وله مستويان: بيئة التعلم المقلوب (تدريس الأقران مقابل الاستقصائي)، مع استراتيجية التنظيم الذاتي للتعلم (البحث عن المساعدة مقابل البحث عن المعلومات)، و

View details →DOI: 10.21608/tesr.2018.124659
Negative / Null Result ReportOpen accessSurgery

Association of Charlson Comorbidity Index and Charlson Fracture Index With Surgical Complications in Distal Radius Fractures: A Retrospective Review

Amber M. Parker, MD, Nicholas Genovese, MD, Alexy Ilchuk, BS et al. · 2026 · Journal of Hand Surgery Global Online

Purpose: To assess and compare the associative value of comorbidity fracture-specific index (CFI) versus the Charlson comorbidity index (CCI) in identifying patients at risk for early complications after open reduction and internal fixation (ORIF) of distal radius fractures (DRFs). Methods: We performed a single-center retrospective cohort study including patients ≥40 years who underwent ORIF for closed DRF using a volar locking plate with a minimum of 1 year follow-up. CCI and CFI scores were calculated based on comorbidities over the prior 15 years. The primary outcome was 90-day postoperati

View details →DOI: 10.1016/j.jhsg.2026.100959
Negative / Null Result ReportOpen accessNeurology. Diseases of the nervous system

Longitudinal evaluation of olfactory function in individuals with Gaucher disease and GBA1 mutation carriers with and without Parkinson's disease

Grisel J. Lopez, Jens Lichtenberg, Nahid Tayebi et al. · 2022 · Frontiers in Neurology

ObjectiveBiallelic mutations in GBA1, which encodes the lysosomal enzyme glucocerebrosidase, cause the lysosomal storage disorder Gaucher disease (GD). In addition, mutations in GBA1 are the most common genetic risk factor for future development of Parkinson's disease (PD). However, most mutation carriers will never develop parkinsonism. Olfactory dysfunction is often a prodromal symptom in patients with PD, appearing many years prior to motor dysfunction. The purpose of this study was to assess olfactory function longitudinally in individuals with and without parkinsonism who carry at least o

View details →DOI: 10.3389/fneur.2022.1039214
Negative / Null Result ReportOpen accessNeoplasms. Tumors. Oncology. Including cancer and carcinogens

Learning curve of transanal minimally invasive surgery for rectal neoplasm

Xingwang Li, Xingwang Li, Shaoqing Guo et al. · 2025 · Frontiers in Oncology

ObjectivesThe field of view through transanal endoscopic provides new treatment approaches for solving complex clinical problems. TAMIS belongs to single-port endoscopic surgery, and the operation is complex. Analyzing the learning curve of TAMIS aims to facilitate its better clinical promotion.MethodsA retrospective cohort study analyzed the clinical data of 58 patients who underwent TAMIS by the same surgeon from January 2018 to October 2024. The learning curve of TAMIS was obtained using the cumulative sum (CUSUM) analysis, and the optimal number of surgeries was determined based on the pea

View details →DOI: 10.3389/fonc.2025.1545589
Negative / Null Result ReportOpen accessOrthopedic surgery

[Translated article] Analysis of the results of anterior cruciate ligament reconstruction associated with high-resistance tape reinforcement (ultra-high molecular weight polyethylene braided material)

A.N. Toro-Ibarguen, E. Sánchez Benito, J.M. Cortés Villar · 2026 · Revista Española de Cirugía Ortopédica y Traumatología

Background and objective: The primary objective is to evaluate the clinical and functional outcomes of tape reinforcement in anterior cruciate ligament (ACL) reconstructions, recording complications, as well as the rate of reinterventions and graft failure. Materials and methods: Retrospective analysis of ACL reconstructions with hamstring (HS) autograft that were reinforced with high-strength tape. We included patients in whom we obtained a graft of HS <8 mm or ≥8 mm of poor quality. Age, sex, body mass index (BMI), and previous activity were recorded. Clinical and functional evaluation were

View details →DOI: 10.1016/j.recot.2025.11.004
Negative / Null Result ReportOpen accessMedicine (General)

Craniofacial Characteristics of Thalassemia Major Patients

Sacide Karakas, Ayfer Metin Tellioglu, Mehmet Bilgin et al. · 2019 · Eurasian Journal of Medicine

Abstract Objective: Thalassemias major are the most common autosomal recessive disorders; they are characterized by anomalies in the synthesis of the beta chains of hemoglobin and are often associated with varying degrees of craniofacial anomalies. The purpose of this study was to evaluate the craniofacial dimensions of β-thalassemia patients and to identify differences by comparing them to those of a control group. Materials and Methods: The study comprised 43 thalassemia major patients and 26 age- and sex- matched healthy control subjects. Anthropometric measurements were performed in six di

View details →DOI: 10.5152/eurasianjmed.2016.150013
Negative / Null Result ReportOpen accessScience

Long bone cross-sectional geometric properties of Later Stone Age foragers and herder–foragers

Michelle E. Cameron, Susan Pfeiffer · 2014 · South African Journal of Science

Diaphyseal cross-sectional geometry can be used to infer activity patterns in archaeological populations. We examined the cross-sectional geometric (CSG) properties of adult Later Stone Age (LSA) herder–forager long bones from the inland lower Orange River Valley of South Africa (n=5 m, 13 f). We then compared their CSG properties to LSA forager adults from the coastal fynbos (n=23 m, 14 f) and forest (n=17 m, 19 f) regions, building on a previous report (Stock and Pfeiffer, 2004). The periosteal mould method was used to quantify total subperiosteal area, torsional strength, bilateral asymmetr

View details →DOI: 10.1590/sajs.2014/20130369
Negative / Null Result ReportOpen accessMedicine

Is pomegranate peels infusion effective for disinfection of toothbrushes?

Priscila Lima de Luna FREIRE, Priscila Hernández CAMPOS, Fabíola Galbiatti CARVALHO et al. · Revista de Odontologia da UNESP

Abstract Introduction Methods of decontamination or sanitization of toothbrushes have been questioned. Objective This study assessed the effectiveness of pomegranate peels infusion as a disinfectant of toothbrushes against Streptococcus mutans. Material and method A sample of 16 schoolchildren aged between 7 and 9 years performed brushing 5 days/week, with a careful brushing once a day. After each day of brushing, the toothbrushes were washed and sprayed with one disinfectant solution. This procedure was repeated for 4 weeks using one of the different solutions per week: distilled water (G1; n

View details →DOI: 10.1590/1807-2577.01416
Methods Dead-EndOpen accessFinance

Predictive Power of ESG Factors for DAX ESG 50 Index Forecasting Using Multivariate LSTM

Manuel Rosinus, Jan Lansky · 2025 · International Journal of Financial Studies

As investors increasingly use Environmental, Social, and Governance (ESG) criteria, a key challenge remains: ESG data is typically reported annually, while financial markets move much faster. This study investigates whether incorporating annual ESG scores can improve monthly stock return forecasts for German DAX-listed firms. We employ a multivariate long short-term memory (LSTM) network, a machine learning model ideal for time series data, to test this hypothesis over two periods: an 8-year analysis with a full set of ESG scores and a 16-year analysis with a single disclosure score. The evalu

View details →DOI: 10.3390/ijfs13030167
Failed Experiment ReportOpen accessDentistry

Evaluating the effectiveness of advanced platelet-rich fibrin, photobiomodulation, pentoxifylline, and Alveogyl in the treatment of alveolar osteitis: a randomized controlled clinical trial

Alper Yuzbasioglu, Cennet Neslihan Eroglu · 2024 · BMC Oral Health

Abstract Background Alveolar osteitis is a type of small-scale osteomyelitis of the alveolar bone that occurs after tooth extraction, the etiology of which remains unknown, and alternative methods are being investigated for its treatment. The aim of this study was to compare the effectiveness of advanced platelet-rich fibrin (A-PRF), photobiomodulation (PBM), and Alveogyl (butamben, idoform, eugenol), which have shown success in the treatment of alveolar osteitis, with that of pentoxifylline (PTX) to determine whether PTX could be an alternative treatment for alveolar osteitis. Methods This st

View details →DOI: 10.1186/s12903-024-05372-6
Failed Experiment ReportOpen accessMedicine

Effect of methylprednisolone treatment on COVID-19: An inverse probability of treatment weighting analysis.

Lorenzo Porta, Sih-Shiang Huang, Chen Wei et al. · 2022 · PLoS ONE

Objectives While corticosteroids have been hypothesized to exert protective benefits in patients infected with SARS-CoV-2, data remain mixed. This study sought to investigate the outcomes of methylprednisone administration in an Italian cohort of hospitalized patients with confirmed SARS-CoV-2 infection. Methods Patients with confirmatory testing for SARS-CoV-2 were retrospectively enrolled from a tertiary university hospital in Milan, Italy from March 1st to April 30th, 2020 and divided into two groups by administration of corticosteroids. Methylprednisolone was administered to patients not r

View details →DOI: 10.1371/journal.pone.0266901
Negative / Null Result ReportOpen accessSurgery

Use of Botulinum Toxin for the Treatment of Raynaud Phenomenon

Manasa H. Kalluri, BS, Armin Edalatpour, MD, Allison J. Seitz, MD et al. · 2026 · Plastic and Reconstructive Surgery, Global Open

Summary:. Raynaud phenomenon (RP) is a vasospastic condition of the digital arteries that can cause pain, skin color changes, and functional limitations. Although botulinum toxin is not currently Food and Drug Administration–approved for the treatment of RP, it has been used in refractory RP and has generally been found to be well tolerated. At our institution, incobotulinumtoxinA (Xeomin) and abobotulinumtoxinA (Dysport) are commonly used. Here, we report our experience with the use of incobotulinumtoxinA (Xeomin) in the hand. We present 2 cases of patients with RP in bilateral hands who pres

View details →DOI: 10.1097/gox.0000000000007424
Negative / Null Result ReportOpen accessAgriculture (General)

Laboratory study of the nutrient release rate for vinasse on sandy soil and three coastal clay soils of Guyana

Clairmont Clementson, Lalita Gopaul · 2020 · International Journal of Recycling of Organic Waste in Agriculture

Purpose Vinasse is produced in large quantities as a by-product from bioethanol production. To ensure environmental sustainability, a beneficial use was sought. Previous study showed that short-term crops grown with vinasse-amended soil provided higher yield than crops treated with chemical NPK (15:15:15) fertilizer. To understand this phenomenon, this study sought to establish the nutrient release rates of vinasse in the agricultural soils of Guyana. Methods Soils representative of the three most common and one futuristic agricultural land in Guyana were fertilized with vinasse, then watered

View details →DOI: 10.30486/ijrowa.2020.1890596.1026
Negative / Null Result ReportOpen accessPsychiatry

A low-threshold sleep intervention for improving sleep quality and well-being

Esther-Sevil Eigl, Laura Krystin Urban-Ferreira, Manuel Schabus et al. · 2023 · Frontiers in Psychiatry

BackgroundApproximately one-third of the healthy population suffer from sleep problems, but only a small proportion of those affected receive professional help. Therefore, there is an urgent need for easily accessible, affordable, and efficacious sleep interventions.ObjectiveA randomized controlled study was conducted to investigate the efficacy of a low-threshold sleep intervention consisting of either (i) sleep data feedback plus sleep education or (ii) sleep data feedback alone in comparison with (iii) no intervention.Material and methodsA total of 100 employees of the University of Salzbur

View details →DOI: 10.3389/fpsyt.2023.1117645
Negative / Null Result ReportOpen accessEducation

Is Teacher Effectiveness Stable Across School Contexts? An Examination of Teachers Who Transfer Into Turnaround Schools

Lam D. Pham · 2022 · AERA Open

Turnaround interventions often require or encourage low-performing schools to replace teachers, assuming that schools will recruit high-performing teachers who remain effective after transferring. However, teacher effectiveness may change after transferring, which could explain why some teacher replacement efforts do not improve student achievement. This paper contributes new information on the stability of teacher effectiveness by examining teachers who transfer into turnaround schools relative to teachers who transfer into low-performing but non-turnaround schools. I examine this issue by us

View details →DOI: 10.1177/23328584221139763
Negative / Null Result ReportOpen accessElectrical engineering. Electronics. Nuclear engineering

Solar energy for liquid wastewater treatment with novel TiO2 supported catalysts

Rui C. Martins, Ângelo Sacras, Sanja Jovanovic et al. · 2022 · Energy Reports

Photocatalytic oxidation is promising technology for removal of recalcitrant pollutants from water. Solar energy can be an interesting radiation source since the operating costs can be lower. However, the use of powder photocatalyst is a major drawback of the technology since suitable separation technologies are required and catalysts recovery is difficult. This work aims to test the suitability of using polymeric supports to immobilize TiO 2 in the reactor and apply it for parabens removal from water by solar photocatalytic oxidation. Polyurethanes (PU) and polydimethylsiloxane (PDMS) membran

View details →DOI: 10.1016/j.egyr.2022.01.196
Negative / Null Result ReportOpen accessChemical technology

Performance-Based Robotic Training in Individuals with Subacute Stroke: Differences between Responders and Non-Responders

Ophélie Pila, Christophe Duret, Typhaine Koeppel et al. · 2023 · Sensors

The high variability of upper limb motor recovery with robotic training (RT) in subacute stroke underscores the need to explore differences in responses to RT. We explored differences in baseline characteristics and the RT dose between responders (ΔFugl-Meyer Assessment (FMA) score ≥ 9 points; n = 20) and non-responders ( n = 16) in people with subacute stroke (mean [SD] poststroke time at baseline, 54 (26) days, baseline FMA score, 23 (17) points) who underwent 16 RT sessions combined with conventional therapies. Baseline characteristics were compared between groups. During RT sessions, the a

View details →DOI: 10.3390/s23094304
Negative / Null Result ReportOpen accessDiseases of the digestive system. Gastroenterology

A randomized control trial to assess the effectiveness of a brief educational intervention on colonoscopy preparation and experience in patients with inflammatory bowel disease

Sumanpreet Kaur, Neha Jindal, Amandeep Kaur et al. · 2025 · BMC Gastroenterology

Abstract Background Colonoscopy is important for diagnosis, assessment and dysplasia screening in inflammatory bowel disease (IBD). A good bowel preparation is required for complete and adequate assessment. Trial design Randomised, assessor blinded parallel arm trial with 1:1 allocation. Methods Patients with IBD undergoing colonoscopy were randomised to control group (standard preparation with written instructions) or experimental group (additional brief video educational intervention). Outcome assessors and colonoscopist were masked to the allocation. Primary outcome was adequacy of bowel pr

View details →DOI: 10.1186/s12876-025-04242-x
Negative / Null Result ReportOpen accessGeriatrics

Absolute and relative reliability of acute effects of aerobic exercise on executive function in seniors

Lars Donath, Sebastian Ludyga, Daniel Hammes et al. · 2017 · BMC Geriatrics

Abstract Background Aging is accompanied by a decline of executive function. Aerobic exercise training induces moderate improvements of cognitive domains (i.e., attention, processing, executive function, memory) in seniors. Most conclusive data are obtained from studies with dementia or cognitive impairment. Confident detection of exercise training effects requires adequate between-day reliability and low day-to-day variability obtained from acute studies, respectively. These absolute and relative reliability measures have not yet been examined for a single aerobic training session in seniors.

View details →DOI: 10.1186/s12877-017-0634-x
Negative / Null Result ReportOpen accessMedicine

How safe and effective is oral analgesia for hip fracture patients? – a prospective cohort study

Zehra Abdul Muhammad, Tashfeen Ahmad · 2025 · Journal of the Pakistan Medical Association

Objective: To assess the safety and pain reduction profile of routinely prescribed oral analgesics in patients of hip fracture surgery. Method: The single-centre, prospective, cohort study was conducted from June 2022 to July 2023 at the Aga Khan University Hospital, Karachi and comprised adult patients of either gender with hip fractures. The analgesics prescribed at the time of hospital discharge and 1-week follow-up were recorded along with pain and adverse effects till the 2-week post-discharge follow-up. Pain was recorded using the visual analogue scale, with the target score set at 3/10.

View details →DOI: 10.47391/jpma.20532
Negative / Null Result ReportOpen accessDentistry

Addition of bone morphogenetic protein type 2 to ascorbate and -glycerophosphate supplementation did not enhance osteogenic differentiation of human adipose-derived stem cells

Ariadne Cristiane Cabral Cruz, Mariana Lúcia Silva, Thiago Caon et al. · 2012 · Journal of Applied Oral Science

Bone morphogenetic protein type 2 (BMP-2) is a potent local factor, which promotes bone formation and has been used as an osteogenic supplement for mesenchymal stem cells. OBJECTIVES: This study evaluated the effect of a recombinant BMP-2 as well as the endogenous BMP-4 and BMP-7 in the osteogenic differentiation of adipose-derived stem cells (ASCs) in medium supplemented with ascorbate and -glycerophosphate. MATERIAL AND METHODS: Human ASCs were treated with osteogenic medium in the presence (ASCs+OM+BMP-2) or absence (ASCs+OM) of BMP-2. The alkaline phosphatase (ALP) activity was determined