Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Han Wu, Xiang Zhao, Fei Wang et al. · 2017 · Frontiers in Immunology
Mumps virus (MuV) infection has high tropism to the testis and usually leads to orchitis, an etiological factor in male infertility. However, MuV replication in testicular cells and the cellular antiviral responses against MuV are not fully understood. The present study showed that MuV infected the majority of testicular cells, including Leydig cells (LC), testicular macrophages, Sertoli cells (SC), and male germ cells (GC). MuV was replicated at relatively high efficiencies in SC compared with LC and testicular macrophages. In contrast, MuV did not replicate in male GC. Notably, testicular ce
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Sindrila Dutta Banik, Nilashis Nandi · 2012 · Journal of Biomolecular Structure and Dynamics
In the present work we report, for the first time, a novel difference in the molecular mechanism of the activation step of aminoacylation reaction between the class I and class II aminoacyl tRNA synthetases (aaRSs). The observed difference is in the mode of nucleophilic attack by the oxygen atom of the carboxylic group of the substrate amino acid (AA) to the αP atom of adenosine triphosphate (ATP). The syn oxygen atom of the carboxylic group attacks the α-phosphorous atom (αP) of ATP in all class I aaRSs (except TrpRS) investigated, while the anti oxygen atom attacks in the case of class II aa
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Kagistia Hana Utami, Nur Amirah Binte Mohammad Yusof, Jing Eugene Kwa et al. · 2020 · Molecular Autism
FXS is the most common genetic cause of intellectual (ID) and autism spectrum disorders (ASD). FXS is caused by loss of FMRP, an RNA-binding protein involved in the translational regulation of a large number of neuronal mRNAs. Absence of FMRP has been shown to lead to elevated protein synthesis and is thought to be a major cause of the synaptic plasticity and behavioural deficits in FXS. The increase in protein synthesis results in part from abnormal activation of key protein translation pathways downstream of ERK1/2 and mTOR signalling. Pharmacological and genetic interventions that attenuate
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Michelle G. Pitts, Tanya Myers‐Morales, Sarah E. F. D’Orazio · 2016 · The Journal of Immunology
Type I IFN (IFN-α/β) is thought to enhance growth of the foodborne intracellular pathogen Listeria monocytogenes by promoting mechanisms that dampen innate immunity to infection. However, the type I IFN response has been studied primarily…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Urko M. Marigorta, Óscar Lao, Ferrán Casals et al. · 2011 · BMC Genomics
BACKGROUND: Searching for associations between genetic variants and complex diseases has been a very active area of research for over two decades. More than 51,000 potential associations have been studied and published, a figure that keeps increasing, especially with the recent explosion of array-based Genome-Wide Association Studies. Even if the number of true associations described so far is high, many of the putative risk variants detected so far have failed to be consistently replicated and are widely considered false positives. Here, we focus on the world-wide patterns of replicability of
View details →Negative / Null Result ReportBiochemistry, Genetics and Molecular Biology
Guohao Xie, Glenn C Duff, L.W. Hall et al. · 2013 · Journal of Animal Science
The effects of bacitracin methylene disalicylate (BMD) and scours on the fecal microbiome, animal performance, and health were studied in Holstein bull calves. Holstein bull calves (n = 150) were obtained from a single source at 12 to 24 h…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
S. Heirbaut, Xiaoping Jing, Barbara Stefańska et al. · 2022 · Journal of Dairy Science
Data on metabolic profiles of blood sampled at d 3, 6, 9, and 21 in lactation from 117 lactations (99 cows) were used for unsupervised k-means clustering. Blood metabolic parameters included -hydroxybutyrate (BHB), nonesterified fatty acids, glucose, insulin-like growth factor-1 (IGF-1) and insulin. Clustering relied on the average and range of the 5 blood parameters of all 4 sampling days. The clusters were labeled as imbalanced (n = 42) and balanced (n = 72) metabolic status based on the values of the blood parameters. Various random forest models were built to predict the metabolic cluster
View details →Negative / Null Result ReportBiochemistry, Genetics and Molecular Biology
Aditi Mathur, Matthew H. Law, Ian L. Megson et al. · 2010 · Psychiatric Genetics
OBJECTIVES: A number of studies have reported a genetic association of the AKT1 gene with schizophrenia, although some have failed to replicate the AKT1 association. This study was undertaken to further explore the AKT1 association with…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Kirandeep Bhullar, Maryam Zarepour, Hongbing Yu et al. · 2015 · Infection and Immunity
Bacterial pathogens produce a number of autotransporters that possess diverse functions. These include the family of serine protease autotransporters of Enterobacteriaceae (SPATEs) produced by enteric pathogens such as Shigella flexneri…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Yuan Gao, Dokyun Lee, Gordon Burtch et al. · 2025 · Proceedings of the National Academy of Sciences
Recent studies suggest large language models (LLMs) can generate human-like responses, aligning with human behavior in economic experiments, surveys, and political discourse. This has led many to propose that LLMs can be used as surrogates…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Francesco Giuseppe De Rosa, Silvia Corcione, Giovanni Di Perri et al. · 2015 · PubMed
Tigecycline, the first member of the glycylcyclines, has been approved for complicated skin and soft tissue infections (cSSTIs) and complicated intra-abdominal infections (cIAIs). It has a wide range of activity against Gram-positive and…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Maximilian L. Würstle, Markus Rehm · 2014 · Journal of Biological Chemistry
The protease caspase-9 is activated on the apoptosome, a multiprotein signal transduction platform that assembles in response to mitochondria-dependent apoptosis initiation. Despite extensive molecular research, the assembly of the holo-apoptosome and the process of caspase-9 activation remain incompletely understood. Here, we therefore integrated quantitative data on the molecular interactions and proteolytic processes during apoptosome formation and apoptosis execution and conducted mathematical simulations to investigate the resulting biochemical signaling, quantitatively and kinetically. I
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Ismaïl Ahmed, Pei‐Chen Lee, Christina M. Lill et al. · 2014 · PLoS Genetics
The etiology of Parkinson disease (PD) involves both genetic susceptibility and environmental exposures. In particular, coffee consumption is inversely associated with PD but the mechanisms underlying this intriguing association are unknown. According to a recent genome-wide gene-environment interaction study, the inverse coffee-PD association was two times stronger among carriers of the T allele of SNP rs4998386 in gene GRIN2A than in homozygotes for the C allele. We attempted to replicate this result in a similarly sized pooled analysis of 2,289 cases and 2,809 controls from four independent
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
William R. Scott, Weihua Zhang, Marie Loh et al. · 2016 · PLoS ONE
South Asians are 1/4 of the world's population and have increased susceptibility to central obesity and related cardiometabolic disease. Knowledge of genetic variants affecting risk of central obesity is largely based on genome-wide association studies of common SNPs in Europeans. To evaluate the contribution of DNA sequence variation to the higher levels of central obesity (defined as waist hip ratio adjusted for body mass index, WHR) among South Asians compared to Europeans we carried out: i) a genome-wide association analysis of >6M genetic variants in 10,318 South Asians with focused analy
View details →Negative / Null Result ReportBiochemistry, Genetics and Molecular Biology
Kang‐Hsi Wu, Chung-Hsing Wang, Yung‐Li Yang et al. · 2010 · PubMed
BACKGROUND: The DNA repair gene XRCC4, a member of the protein family involved in non-homologous end-joining repair pathway, plays a major role in repairing DNA double-strand breaks. XRCC4 is important in maintaining the overall genome…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Jin‐Yi Wan, Chong‐Zhi Wang, Qi‐Hui Zhang et al. · 2016 · Biomedical Chromatography
Abstract After ingestion of ginseng, the bioavailability of its parent compounds is low and enteric microbiota plays an important role in parent compound biotransformation to their metabolites. Diet type can influence the enteric…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Shahzad Ahmad, Adelina Orellana, Isabelle Köhler et al. · 2020 · Alzheimer s Research & Therapy
BACKGROUND: Lysophosphatidic acids (LPAs) are bioactive signaling phospholipids that have been implicated in Alzheimer's disease (AD). It is largely unknown whether LPAs are associated with AD pathology and progression from mild cognitive impairment (MCI) to AD. METHODS: The current study was performed on cerebrospinal fluid (CSF) and plasma samples of 182 MCI patients from two independent cohorts. We profiled LPA-derived metabolites using liquid chromatography-mass spectrometry. We evaluated the association of LPAs with CSF biomarkers of AD, Aβ-42, p-tau, and total tau levels overall and stra
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Mark P. Purdue, Yuanqing Ye, Zhaoming Wang et al. · 2013 · Cancer Epidemiology Biomarkers & Prevention
Genome-wide association studies (GWAS) of renal cell carcinoma (RCC) in populations of European ancestry have identified four susceptibility loci. No GWAS has been conducted among African Americans (AA), who experience a higher incidence…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Rebecca C. Richmond, Carlos Sillero‐Rejon, Jasmine Khouja et al. · 2021 · Clinical Epigenetics
Abstract Background Little evidence exists on the health effects of e-cigarette use. DNA methylation may serve as a biomarker for exposure and could be predictive of future health risk. We aimed to investigate the DNA methylation profile of e-cigarette use. Results Among 117 smokers, 117 non-smokers and 116 non-smoking vapers, we evaluated associations between e-cigarette use and epigenome-wide methylation from saliva. DNA methylation at 7 cytosine-phosphate-guanine sites (CpGs) was associated with e-cigarette use at p < 1 × 10 –5 and none at p < 5.91 × 10 –8 . 13 CpGs were associated wi
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
I. Krzyzewska, Judith Ensink, Laura Nawijn et al. · 2018 · European Journal of Human Genetics
Posttraumatic stress disorder (PTSD) is a debilitating psychiatric disorder that may develop after a traumatic event. Here we aimed to identify epigenetic and genetic loci associated with PTSD. We included 73 traumatized police officers…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
W-J Xiao, J-W He, H Zhang et al. · 2010 · International Journal of Obesity
OBJECTIVE: Arachidonate 12-lipoxygenase (ALOX12) is a member of the lipoxygenase superfamily, which catalyzes the incorporation of molecular oxygen into polyunsaturated fatty acids. The products of ALOX12 reactions serve as endogenous…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Xiaona Yang, Hong Guo, Min Zou · 2026 · Molecular Biomedicine
Inflammatory bowel disease (IBD) is a heterogeneous group of disorders characterized primarily by chronic relapsing intestinal inflammation, encompassing Crohn's disease (CD) and ulcerative colitis (UC), affecting individuals across age groups with variable clinical manifestations. With the advancement of global industrialization, its incidence continues to rise, particularly in newly industrialized regions, which not only severely impairs patients' quality of life but also emerges as a major public health concern threatening digestive system health, accompanied by a substantial healthcare bur
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Sean M. Carroll, Katherine S. Xue, Christopher J. Marx · 2014 · BMC Microbiology
BACKGROUND: A common assumption of microorganisms is that laboratory stocks will remain genetically and phenotypically constant over time, and across laboratories. It is becoming increasingly clear, however, that mutations can ruin strain integrity and drive the divergence or "domestication" of stocks. Since its discovery in 1960, a stock of Methylobacterium extorquens AM1 ("AM1") has remained in the lab, propagated across numerous growth and storage conditions, researchers, and facilities. To explore the extent to which this lineage has diverged, we compared our own "Modern" stock of AM1 to a
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Eamonn P. Culligan, Julian R. Marchesi, Colin Hill et al. · 2014 · Frontiers in Microbiology
In the current study, a number of salt-tolerant clones previously isolated from a human gut metagenomic library were screened using Phenotype MicroArray (PM) technology to assess their functional capacity. PM's can be used to study gene function, pathogenicity, metabolic capacity and identify drug targets using a series of specialized microtitre plate assays, where each well of the microtitre plate contains a different set of conditions and tests a different phenotype. Cellular respiration is monitored colorimetrically by the reduction of a tetrazolium dye. One clone, SMG 9, was found to be po
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Heini Sánez Tähtisalo, Sanni Ruotsalainen, Nina Mars et al. · 2020 · Scientific Reports
Polygenic risk scores (PRSs) for essential hypertension, calculated from > 900 genomic loci, were recently found to explain a significant fraction of hypertension heritability and complications. To investigate whether variation of hypertension PRS also captures variation of antihypertensive drug responsiveness, we calculated two different PRSs for both systolic and diastolic blood pressure: one based on the top 793 independent hypertension-associated single nucleotide polymorphisms and another based on over 1 million genome-wide variants. Using our pharmacogenomic GENRES study comprising four
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Masaru Sekijima, Hiroshi Takeda, Katsuaki Yasunaga et al. · 2010 · Journal of Radiation Research
We investigated the mechanisms by which radiofrequency (RF) fields exert their activity, and the changes in both cell proliferation and the gene expression profile in the human cell lines, A172 (glioblastoma), H4 (neuroglioma), and IMR-90…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Boshu Ru, Dingcheng Li, Yueqi Hu et al. · 2019 · IEEE Transactions on NanoBioscience
Serendipitous drug usage refers to the unexpected relief of comorbid diseases or symptoms when taking medication for a different known indication. Historically, serendipity has contributed significantly to identifying many new drug…
View details →Negative / Null Result ReportBiochemistry, Genetics and Molecular Biology
Ying Shen, Yuanlong Yan, Yunqiang Liu et al. · 2013 · Human Molecular Genetics
AZFc deletions cause a significant phenotypic heterogeneity with respect to spermatogenesis; however, the reason for this is poorly understood. Recently, testis-specific protein Y-encoded 1 (TSPY1) copy number variation (CNV) was…
View details →Negative / Null Result ReportBiochemistry, Genetics and Molecular Biology
Bo Xi, Yue Shen, Kathleen H. Reilly et al. · 2012 · Clinical Endocrinology
OBJECTIVE: Recent genome-wide association studies have identified a few single nucleotide polymorphisms (SNPs), which are associated with body mass index (BMI)/obesity. This study aimed to examine the identified associations among a…
View details →Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Oriah Mioduser, Eli Goz, Tamir Tuller · 2017 · BMC Genomics
BACKGROUND: Viruses undergo extensive evolutionary selection for efficient replication which effects, among others, their codon distribution. In the current study, we aimed at understanding the way evolution shapes the codon distribution in early vs. late viral genes in terms of their expression during different stages in the viral replication cycle. To this end we analyzed 14 bacteriophages and 11 human viruses with available information about the expression phases of their genes. RESULTS: We demonstrated evidence of selection for distinct composition of synonymous codons in early and late vi
View details →