Negative / Null Result ReportOpen accessMedicine
Natalie Hofmann, Felista Mwingira, Seif Shekalaghe et al. · 2015 · PLoS Medicine
BACKGROUND: Planning and evaluating malaria control strategies relies on accurate definition of parasite prevalence in the population. A large proportion of asymptomatic parasite infections can only be identified by surveillance with molecular methods, yet these infections also contribute to onward transmission to mosquitoes. The sensitivity of molecular detection by PCR is limited by the abundance of the target sequence in a DNA sample; thus, detection becomes imperfect at low densities. We aimed to increase PCR diagnostic sensitivity by targeting multi-copy genomic sequences for reliable det
View details →DOI: 10.1371/journal.pmed.1001788Cited by 464 Negative / Null Result ReportOpen accessMedicine
Amy J. Schuh, Melissa J. Ward, Andrew Brown et al. · 2013 · PLoS neglected tropical diseases
The circulation of vector-borne zoonotic viruses is largely determined by the overlap in the geographical distributions of virus-competent vectors and reservoir hosts. What is less clear are the factors influencing the distribution of virus-specific lineages. Japanese encephalitis virus (JEV) is the most important etiologic agent of epidemic encephalitis worldwide, and is primarily maintained between vertebrate reservoir hosts (avian and swine) and culicine mosquitoes. There are five genotypes of JEV: GI-V. In recent years, GI has displaced GIII as the dominant JEV genotype and GV has re-emerg
View details →DOI: 10.1371/journal.pntd.0002411Cited by 136 Negative / Null Result ReportOpen accessAgricultural and Biological Sciences
Tariq M. Butt, Bethany P. J. Greenfield, Carolyn Greig et al. · 2013 · PLoS ONE
Metarhizium anisopliae, a fungal pathogen of terrestrial arthropods, kills the aquatic larvae of Aedes aegypti, the vector of dengue and yellow fever. The fungus kills without adhering to the host cuticle. Ingested conidia also fail to germinate and are expelled in fecal pellets. This study investigates the mechanism by which this fungus adapted to terrestrial hosts kills aquatic mosquito larvae. Genes associated with the M. anisopliae early pathogenic response (proteinases Pr1 and Pr2, and adhesins, Mad1 and Mad2) are upregulated in the presence of larvae, but the established infection proces
View details →DOI: 10.1371/journal.pone.0081686Cited by 130 Negative / Null Result ReportOpen accessMedicine
Marco Marklewitz, Florian Zirkel, Innocent B. Rwego et al. · 2013 · Journal of Virology
Bunyaviruses are the largest known family of RNA viruses, infecting vertebrates, insects, and plants. Here we isolated three novel bunyaviruses from mosquitoes sampled in Côte d'Ivoire, Ghana, and Uganda. The viruses define a highly…
View details →DOI: 10.1128/jvi.01862-13Cited by 101 Negative / Null Result ReportOpen accessMedicine
Kelly A. Liebman, Steven T. Stoddard, Amy C. Morrison et al. · 2012 · PLoS neglected tropical diseases
BACKGROUND: Knowledge of spatial patterns of dengue virus (DENV) infection is important for understanding transmission dynamics and guiding effective disease prevention strategies. Because movement of infected humans and mosquito vectors plays a role in the spread and persistence of virus, spatial dimensions of transmission can range from small household foci to large community clusters. Current understanding is limited because past analyses emphasized clinically apparent illness and did not account for the potentially large proportion of inapparent infections. In this study we analyzed both c
View details →DOI: 10.1371/journal.pntd.0001472Cited by 91 Negative / Null Result ReportOpen accessMedicine
John C. Morrill, Tetsuro Ikegami, Naoko Yoshikawa-Iwata et al. · 2010 · PLoS ONE
BACKGROUND: Rift Valley fever virus (RVFV), a member of the genus Phlebovirus within the family Bunyaviridae, is a negative-stranded RNA virus with a tripartite genome. RVFV is transmitted by mosquitoes and causes fever and severe hemorrhagic illness among humans, while in livestock it causes fever and high abortion rates. METHODOLOGY/PRINCIPAL FINDINGS: Sequence analysis showed that a wild-type RVFV ZH501 preparation consisted of two major viral subpopulations, with a single nucleotide heterogeneity at nucleotide 847 of M segment (M847); one had a G residue at M847 encoding glycine in a major
View details →DOI: 10.1371/journal.pone.0009986Cited by 50 Negative / Null Result ReportOpen accessMedicine
Jessica J. Harrison, David Warrilow, Breeanna J. McLean et al. · 2016 · Viruses
The discovery and characterisation of new mosquito-borne viruses provides valuable information on the biodiversity of vector-borne viruses and important insights into their evolution. In this study, a broad-spectrum virus screening system, based on the detection of long double-stranded RNA in inoculated cell cultures, was used to investigate the presence of novel viruses in mosquito populations of northern Australia. We detected and isolated a new virus (tentatively named Parry's Lagoon virus, PLV) from Culex annulirostris, Culex pullus, Mansonia uniformis and Aedes normanensis mosquitoes that
View details →DOI: 10.3390/v8050141Cited by 46 Negative / Null Result ReportOpen accessMedicine
Ricardo Parreira, Shelley Cook, Ângela Celis de Almeida Lopes et al. · 2012 · Virus Research
We describe the full genetic characterization of an insect-specific flavivirus (ISF) from Culex theileri (Theobald) mosquitoes collected in Portugal. This represents the first isolation and full characterization of an ISF from Portuguese mosquitoes. The virus, designated CTFV, for Culex theileri flavivirus, was isolated in the C6/36 Stegomyia albopicta (=Aedes albopictus) cell line, and failed to replicate in vertebrate (Vero) cells in common with other ISFs. The CTFV genome encodes a single polyprotein with 3357 residues showing all the features expected for those of flaviviruses. Phylogeneti
View details →DOI: 10.1016/j.virusres.2012.04.010Cited by 43 Negative / Null Result ReportOpen accessMedicine
Albert J. Auguste, Rose M. Langsjoen, Danielle L. Porier et al. · 2021 · Virology
We describe the isolation and characterization of a novel insect-specific flavivirus (ISFV), tentatively named Aripo virus (ARPV), that was isolated from Psorophora albipes mosquitoes collected in Trinidad. The ARPV genome was determined…
View details →DOI: 10.1016/j.virol.2021.07.004Cited by 27 Negative / Null Result ReportOpen accessAgricultural and Biological Sciences
Arley Calle-Tobón, Juliana Pérez-Pérez, Nicolás Forero-Pineda et al. · 2022 · PLoS ONE
Aedes spp. comprise the primary group of mosquitoes that transmit arboviruses such as dengue, Zika, and chikungunya viruses to humans, and thus these insects pose a significant burden on public health worldwide. Advancements in next-generation sequencing and metagenomics have expanded our knowledge on the richness of RNA viruses harbored by arthropods such as Ae. aegypti and Ae. albopictus. Increasing evidence suggests that vector competence can be modified by the microbiome (comprising both bacteriome and virome) of mosquitoes present in endemic zones. Using an RNA-seq-based metataxonomic app
View details →DOI: 10.1371/journal.pone.0263143Cited by 25 Negative / Null Result ReportMedicine
Nathalie Charlier, Andrew D. Davidson, Kai Dallmeier et al. · 2010 · Journal of General Virology
Chimeric yellow fever virus 17D (YFV-17D) and dengue virus type 2 (DENV2) carrying the surface proteins of Modoc virus (MODV), a not-known-vector (NKV) flavivirus, replicated efficiently in mammalian (Vero-B) and mosquito (C6/36) cells,…
View details →DOI: 10.1099/vir.0.019851-0Cited by 24 Negative / Null Result ReportOpen accessMedicine
Geovani de Oliveira Ribeiro, Antônio Charlys da Costa, Danielle Elise Gill et al. · 2021 · Scientific Reports
Classical insect-flaviviruses (cISFVs) and dual host-related insect-specific flavivirus (dISFV) are within the major group of insect-specific flavivirus. Remarkably dISFV are evolutionarily related to some of the pathogenic flavivirus, such as Zika and dengue viruses. The Evolutionary relatedness of dISFV to flavivirus allowed us to investigate the evolutionary principle of host adaptation. Additionally, dISFV can be used for the development of flavivirus vaccines and to explore underlying principles of mammalian pathogenicity. Here we describe the genetic characterization of a novel putative
View details →DOI: 10.1038/s41598-021-83879-6Cited by 21 Negative / Null Result ReportOpen accessMedicine
Natalie A. Prow, Marcus G. Mah, Joshua M. Deerain et al. · 2018 · Journal of General Virology
Liao ning virus (LNV) was first isolated in 1996 from mosquitoes in China, and has been shown to replicate in selected mammalian cell lines and to cause lethal haemorrhagic disease in experimentally infected mice. The first detection of…
View details →DOI: 10.1099/jgv.0.001038Cited by 19 Negative / Null Result ReportOpen accessMedicine
Jittawadee Murphy, Walter R. Weiss, David J. Fryauff et al. · 2014 · Malaria Journal
BACKGROUND: When rhesus monkeys (Macaca mulatta) are used to test malaria vaccines, animals are often challenged by the intravenous injection of sporozoites. However, natural exposure to malaria comes via mosquito bite, and antibodies can neutralize sporozoites as they traverse the skin. Thus, intravenous injection may not fairly assess humoral immunity from anti-sporozoite malaria vaccines. To better assess malaria vaccines in rhesus, a method to challenge large numbers of monkeys by mosquito bite was developed. METHODS: Several species and strains of mosquitoes were tested for their ability
View details →DOI: 10.1186/1475-2875-13-215Cited by 15 Negative / Null Result ReportOpen accessMedicine
Lu Zhao, Caroline Mwaliko, Evans Atoni et al. · 2019 · Frontiers in Microbiology
Globally, mosquitoes are known to be competent vectors to various arboviruses that cause serious and debilitating diseases to humans and animals. Conversely, mosquitoes harbor a wide array of insect specific viruses (ISVs) that are generally neglected. Extensive characterization of these ISVs is important in understanding their persistence infection effect on host behavior and arbovirus transmission. Herein, we report first time isolation of Tanay virus (TANAV) isolate YN15_103_01 in Anopheles sinensis mosquitoes from Yunnan Province, China. Phylogenetically, the isolate’s nucleotide identity
View details →DOI: 10.3389/fmicb.2019.01963Cited by 15 Negative / Null Result ReportOpen accessMedicine
Muhammad Jasim Uddin, Willy W. Suen, Angela M. Bosco‐Lauth et al. · 2016 · Veterinary Research
West Nile virus (WNV) is one of the most common causes of epidemic viral encephalitis in horses worldwide. Peripheral blood mononuclear cells (PBMCs) are amongst the first to encounter the virus following a mosquito bite. This study aimed to elucidate the transcription kinetics of cytokine, Toll-like receptor (TLRs) and TLRs-associated genes following WNV challenge of equine PBMCs. PBMCs were challenged with an Australian strain of WNV (WNVNSW2011) and transcriptomes were quantified at 2, 6, 12 and 24 h post-infection (pi) using qRT-PCR. Type I and II interferons (IFNα, β and γ) mRNA transcrip
View details →DOI: 10.1186/s13567-016-0347-8Cited by 11 Failed Experiment ReportOpen accessMedicine
Anne Kopp, Alexandra Hübner, Florian Zirkel et al. · 2019 · Viruses
. Orthobunyaviruses and pacuviruses are mainly transmitted by blood-feeding insects and infect a variety of vertebrates whereas herbeviruses and shangaviruses have a host range restricted to insects. Here, we tested mosquitoes from a tropical rainforest in Mexico for infections with peribunyaviruses. We identified and characterized two previously unknown viruses, designated Baakal virus (BKAV) and Lakamha virus (LAKV). Sequencing and de novo assembly of the entire BKAV and LAKV genomes revealed that BKAV is an orthobunyavirus and LAKV is likely to belong to a new genus. LAKV was almost equidis
View details →DOI: 10.3390/v11090832Cited by 11 Negative / Null Result ReportOpen accessInfectious and parasitic diseases
Jenna R. Davidson, Isra Wahid, Rusdiyah Sudirman et al. · 2019 · Parasites & Vectors
Abstract Background Sampling methodologies for mosquitoes that are capable of transmitting vector-borne infectious diseases provide critical information on entomological endpoints. Reliable and meaningful field data is vital to the understanding of basic vector biology as well as disease transmission. Various traps take advantage of different vector behaviors and are inevitably subject to sampling biases. This study represents the first comparison of kelambu traps (KT) to barrier screens (BS), barrier screens with eaves (BSE) and indoor and outdoor human landing catches (HLCs). Methods Two tra
View details →DOI: 10.1186/s13071-019-3649-7 Negative / Null Result ReportMedicine
Wang, Wang, Luo et al. · 2026 · Frontiers in veterinary science
This study aimed to assess the effectiveness of intelligent mosquito surveillance devices implemented in Zhejiang Province and to compare these metrics with those obtained from traditional surveillance methods. Intelligent surveillance…
View details →DOI: 10.3389/fvets.2026.1831713 Negative / Null Result Report
Palatini U, Dabo S, Rosa-Villegas A et al. · 2026 · Preprint
ABSTRACT Billions of people each year are at risk from infection by dengue, Zika, yellow fever, and chikungunya viruses, which are transmitted by female Aedes aegypti mosquitoes. Mosquitoes themselves are infected by these arboviruses, but…
View details →DOI: 10.64898/2026.06.10.731349 Negative / Null Result ReportMedicine
Kelley AT, Boger R · 2025 · EcoHealth
Community gardens in urban Brooklyn, New York are social spaces that provide food sovereignty to many New Yorkers, but the arrival of the mosquito Aedes albopictus and longtime presence of Culex pipiens makes socializing in these spaces…
View details →DOI: 10.1007/s10393-025-01739-5 Negative / Null Result Report
Tornero I, Barta B, Hamer AJ et al. · 2026 · Preprint
ABSTRACT Mosquitoes in urban environments are considered unpleasant by citizens, and they can represent serious health risks as disease vectors. Invasive Aedes species are continuously spreading in Europe and have been increasingly…
View details →DOI: 10.64898/2026.04.07.716928 Negative / Null Result ReportMedicine
Taylor, Roberts, Rayner · 2026 · Viruses
Chikungunya virus (CHIKV) is an alphavirus that infects dermal fibroblasts as a primary target cell during natural mosquito-borne transmission. While primary human dermal fibroblasts (hDFs) have been implicated as a key source of type I…
View details →DOI: 10.3390/v18050503 Negative / Null Result ReportMedicine
Karalyan, Ross, Bayramyan et al. · 2026 · Acta tropica
Mosquito-specific viruses (MSVs) have garnered increasing interest, particularly those phylogenetically related to arboviruses. Among them, giant viruses of the phylum Nucleocytoviricota, such as Marseillevirus, have been detected in…
View details →DOI: 10.1016/j.actatropica.2026.108185 Negative / Null Result ReportMedicine
Lee, Kirby, Brown et al. · 2026 · Parasites & vectors
While mosquito vector-borne disease (VBD) risk remains low in Northern Europe, surveillance is needed to monitor potential vector species. However, most mosquito trapping methods have been evaluated only in areas of high mosquito density,…
View details →DOI: 10.1186/s13071-026-07478-y Negative / Null Result ReportOpen accessPublic aspects of medicine
Juliana Cavalcanti Correia, Rosângela Maria Rodrigues Barbosa, Cláudia Maria Fontes de Oliveira et al. · 2012 · Revista de Saúde Pública
OBJECTIVE: Analyse how basic sanitation conditions, water supply and housing conditions affect the concentration of Culex quinquefasciatus METHODS: Populations of C. quinquefasciatus in 61 houses in the municipality of Olinda, PE, were monitored between October 2009 and October 2010. Observations were carried out in homes without the presence of preferred breeding sites in order to identify characteristics that may be aggravating factors for the development of the mosquito. Five aggravating factors were analysed: vegetation cover surrounding the home, number of residents/home, water storage, s
View details →DOI: 10.1590/s0034-89102013005000010 Negative / Null Result ReportOpen accessPublic aspects of medicine
Santos Luciana Urbano dos, Andrade Carlos Fernando S. de · 1997 · Revista de Saúde Pública
INTRODUCTION: Cyclopid copepods are known to be good mosquito controllers, specially as regards the larvae of the dengue vectors Aedes aegypti and Ae. albopictus. MATERIAL AND METHOD: The objective of the study was to survey the local copepod fauna and search for new strains of M. longisetus var. longisetus, comparing the potential of the samples found with the current strain ML-01 against Ae. albopictus larvae, under laboratory conditions. Eleven bodies of water in Campinas, SP, Brazil, were screened for copepods by collecting 1.5 l of water from each of then. The predatory potential of adult
Negative / Null Result ReportOpen accessMedicine
Chen C, Qualls WA, Xue RD et al. · 2026 · Journal of economic entomology
The sterile insect technique (SIT) is increasingly used as an alternative or supplemental tool in the integrated mosquito management toolbox to protect human health worldwide. SIT programs targeting mosquitoes such as Aedes aegypti (L.)…
View details →DOI: 10.1093/jee/toaf030 Negative / Null Result ReportOpen accessBiotechnology
Enkelejda Velo, Fabrizio Balestrino, Fabrizio Balestrino et al. · 2022 · Frontiers in Bioengineering and Biotechnology
The pathogen transmitting Aedes albopictus mosquito is spreading rapidly in Europe, putting millions of humans and animals at risk. This species is well-established in Albania since its first detection in 1979. The sterile insect technique (SIT) is increasingly gaining momentum worldwide as a component of area-wide-integrated pest management. However, estimating how the sterile males will perform in the field and the size of target populations is crucial for better decision-making, designing and elaborating appropriate SIT pilot trials, and subsequent large-scale release strategies. A mark-rel
View details →DOI: 10.3389/fbioe.2022.833698 