Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Charles R. Dunlop, Yann Wallez, Timothy Isaac Johnson et al. · 2020 · British Journal of Cancer
BACKGROUND: Personalised medicine strategies may improve outcomes in pancreatic ductal adenocarcinoma (PDAC), but validation of predictive biomarkers is required. Having developed a clinical trial to assess the ATR inhibitor, AZD6738, in combination with gemcitabine (ATRi/gem), we investigated ATM loss as a predictive biomarker of response to ATRi/gem in PDAC. METHODS: Through kinase inhibition, siRNA depletion and CRISPR knockout of ATM, we assessed how ATM targeting affected the sensitivity of PDAC cells to ATRi/gem. Using flow cytometry, immunofluorescence and immunoblotting, we investigate
View details →DOI: 10.1038/s41416-020-1016-2Cited by 63 Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology
Raya Kh. Yashooa, Ari Q. Nabi, Shukur Wasman Smail et al. · 2026 · Frontiers in Neurology
CRISPR-Cas genome-editing technologies have emerged as powerful tools for precise DNA and RNA modulation, offering promising therapeutic strategies for neurodegenerative disorders such as Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease (HD), and amyotrophic lateral sclerosis (ALS). This review critically evaluates current CRISPR/Cas applications in neurodegeneration, with emphasis on mechanistic insights, therapeutic outcomes, and translational feasibility. Preclinical and early translational studies demonstrate that CRISPR-Cas platforms can correct pathogenic mutation
View details →DOI: 10.3389/fneur.2025.1737468Cited by 13 Negative / Null Result ReportOpen accessAgricultural and Biological Sciences
Zahra Ghorbanzadeh, Bahman Panahi, Leila Purhang et al. · 2025 · Agronomy
Developing climate-resilient and high-quality cotton cultivars remains an urgent challenge, as the key target traits yield, fibre properties, and stress tolerance are highly polygenic and strongly influenced by genotype–environment interactions. Recent advances in chromosome-scale genome assemblies, pan-genomics, and haplotype-resolved resequencing have greatly enhanced the capacity to identify causal variants and recover non-reference alleles linked to fibre development and environmental adaptation. Parallel progress in functional genomics and precision genome editing, particularly CRISPR/Cas
View details →DOI: 10.3390/agronomy15102393Cited by 10 Negative / Null Result Report
Juihsuan Chou, Nazanin Esmaeili Anvar, Reem Elghaish et al. · 2025 · Genome Biology
Abstract Genetic screens offer a promising strategy for identifying tumor-specific therapeutic targets, but single-gene knockout screens often miss functionally redundant paralogs. Multiplex Cas9 and Cas12a CRISPR systems have been…
View details →DOI: 10.1186/s13059-025-03660-0Cited by 4 Negative / Null Result Report
Chen J, Hou S, Feng R et al. · 2023 · Preprint
CRISPR homing gene drive is a potent technology with considerable potential for managing populations of medically and agriculturally significant insects. It induces a bias in the inheritance of the drive allele in progeny, rapidly…
View details →DOI: 10.1101/2023.11.29.569221 Negative / Null Result Report
Schwartz MA, Lue D, Mutaher M et al. · 2026 · Preprint
Stem-like TCF7+ CD8 T cells sustain anti-tumor responses and support immune checkpoint blockade. We systematically identified regulators of this cell state using genome-wide CRISPR screens in primary T cells in vitro . Using random…
View details →DOI: 10.64898/2026.06.14.732162 Negative / Null Result ReportMedicine
Zaki HF, Bishri J, Abdul Muqtadir M et al. · 2026 · Experimental eye research
Background Cone-rod dystrophy (CORD) and achromatopsia (ACHM) are inherited retinal dystrophies for which conventional adeno-associated virus (AAV) gene augmentation has important limitations, particularly in autosomal-dominant…
View details →DOI: 10.1016/j.exer.2026.111119 Negative / Null Result ReportOpen access
Hoyle C, Pino RD, Lai SM et al. · 2025 · Preprint
Interleukin (IL)-1α is a pro-inflammatory member of the IL-1 cytokine superfamily and is important for inflammatory responses to infection and injury. Unlike pro-IL-1β, pro-IL-1α is mainly localised to the nucleus upon expression. This is…
View details →DOI: 10.1101/2025.09.30.679460 Negative / Null Result ReportOpen accessVeterinary medicine
Dipankar Sardar, Md. Taslim Hossain, Rudranil Baidya et al. · 2024 · Veterinary Integrative Sciences
Gene editing in large animals like buffalo is challenged by mosaicism, where cells contain both wild-type and mutant alleles, complicating the creation of genetically modified F0 animals in a single step. Traditionally, electroporation is performed on zygotes post in vitro fertilization (IVF), but mature oocytes’ higher permeability suggests earlier intervention might reduce mosaicism and enhance editing efficiency. We hypothesized that the timing of electroporation before in vitro fertilization (IVF) can increase the rates of biallelic mutation for multiple gene knockout as the permeability o
View details →DOI: 10.12982/vis.2025.069 Negative / Null Result ReportOpen accessMedicine
Ekrami A, Taheri B, Daneshfar S et al. · 2025 · BMC infectious diseases
BACKGROUND: So far, few studies have examined the association between CRISPR-Cas and antibiotic resistance in Shigella isolates. Hence, this study sought to address this issue in Shigella species isolated from stool samples of patients…
View details →DOI: 10.1186/s12879-025-12100-0 Negative / Null Result Report
Khwanbua E, Lappe RR, Bierl AA et al. · 2026 · Preprint
Plant virus-based gRNA delivery systems offer a rapid alternative to stable transformation for CRISPR-mediated genome editing, but potyvirus-based platforms in Cas9-expressing plants are still underexplored. Here, we developed a turnip…
View details →DOI: 10.64898/2026.04.22.720221 Negative / Null Result ReportMedicine
Chu NH, Le HD, Quach NT et al. · 2026 · Biochemical genetics
The optical purity of lactic acid is a critical parameter for producing high-performance polylactic acid (PLA). To investigate the genetic basis of stereospecific lactic acid biosynthesis, the present study aimed to functionally…
View details →DOI: 10.1007/s10528-026-11374-6 Negative / Null Result Report
Jerabek S, Kim J, Sung J et al. · 2026 · Preprint
ABSTRACT Cas9-based tools enable the introduction of genetic lesions to investigate DNA repair outcomes and edit the genome at disease-relevant loci. DNA double-strand breaks (DSBs) induced by CRISPR/Cas9 result in frequent aneuploidy and…
View details →DOI: 10.64898/2026.05.30.728989 Negative / Null Result ReportOpen accessMedicine
An SY, Kim I, Hong SH et al. · 2026 · Protein science : a publication of the Protein Society
Anti-CRISPR (Acr) proteins are phage-encoded anti-defense factors that suppress CRISPR-Cas immunity in bacteria. AcrIIA8 was previously identified as an inhibitor of Streptococcus pyogenes Cas9 (SpyCas9) through functional assays of…
View details →DOI: 10.1002/pro.70651 