Influence of Electroporation Timing on CRISPR/Cas-Mediated Multiple Gene Editing in Buffalo Embryos
Dipankar Sardar; Md. Taslim Hossain; Rudranil Baidya; Adnan Habib · 2024 · Veterinary Integrative Sciences
WASTE classifies this as Negative / Null Result Report · AI classification, approximate
The study found no significant effect — useful as a negative control or null benchmark for your own design.
Abstract
Gene editing in large animals like buffalo is challenged by mosaicism, where cells contain both wild-type and mutant alleles, complicating the creation of genetically modified F0 animals in a single step. Traditionally, electroporation is performed on zygotes post in vitro fertilization (IVF), but mature oocytes’ higher permeability suggests earlier intervention might reduce mosaicism and enhance editing efficiency. We hypothesized that the timing of electroporation before in vitro fertilization (IVF) can increase the rates of biallelic mutation for multiple gene knockout as the permeability o
Abstract by Dipankar Sardar; Md. Taslim Hossain; Rudranil Baidya; Adnan Habib, Veterinary Integrative Sciences (2024) — licensed CC BY 4.0.
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Metadata source: DOAJ · DOI 10.12982/vis.2025.069