455 real negative results, null findings, and replication failures in Biochemistry, Genetics and Molecular Biology · Negative / Null Result Report. Search the index →
WASTE indexes published research — it does not host or republish full papers. Each entry is a metadata record compiled from open scholarly databases; the abstract is shown in full only where the paper is openly licensed, otherwise a short excerpt under fair use. Classifications are automated and approximate.
Jennifer L. Havens, Sergei L. Kosakovsky Pond, Jordan D. Zehr et al. · 2026 · Cell
Using a phylogenetic framework to characterize natural selection, we investigate the hypothesis that zoonotic viruses require adaptation prior to zoonosis to sustain human-to-human transmission. Examining the zoonotic emergence of Ebola…
View details →Lei Li, Hongchao Huang, Yin He et al. · 2025 · Military Medical Research
Sepsis is a dysregulated host response to infection that frequently results in fatal multiple organ dysfunction. Despite advances in clinical identification and management, both its incidence and mortality have remained persistently high. Emerging evidence indicates that cell-free DNA (cfDNA), as a novel biomarker and molecular therapeutic target, holds promise for improving the clinical management of sepsis. cfDNA refers to DNA fragments present in body fluids, including naked DNA, membrane-coated DNA, nucleosomes, and neutrophil extracellular traps (NETs). cfDNA is released from host cells o
View details →Tahrim Ramzan, Muhammad Shahbaz, Farooq Ahmad et al. · 2026 · New Zealand Journal of Crop and Horticultural Science
Salinity stress is a major abiotic factor that inhibits plant growth and yield. This study's objective was to evaluate how foliar applications of melatonin and ascorbic acid affected canola varieties under salt stress. The treatments of…
View details →Jayeshkumar Kanani · 2025 · The Egyptian Journal of Neurosurgery : the official publication of the Egyptian Society of Neurological Surgeons/Egyptian journal of neurosurgery
Abstract Background Hemangioblastomas are rare, slow-growing benign neoplasms predominantly affecting the central nervous system (CNS). Despite their benign nature, they pose diagnostic challenges due to their diverse clinical manifestations and radiological features. Case presentation. A 40-year-old male presented with chronic headaches and diplopia persisting for 8 years. Initial MRI revealed two cystic lesions in the cerebellum, causing compression and herniation. Surgical removal of one cyst yielded inconclusive results. Follow-up MRIs showed cyst growth and syrinx development. The patient
View details →Wanting Zhang, Yuhang Xiang, Chen Lu et al. · 2026 · Molecular Biomedicine
The extracellular matrix (ECM) forms a dynamic structure around cells, providing environmental cues, mechanical support, and tissue protection. It is composed of fibrous proteins, glycosaminoglycans (GAGs), proteoglycans, and glycoproteins. The molecular, physical, and mechanical properties of the ECM regulate the motility, survival, and function of immune cells. In most cancers, inflammatory cytokines and proteases-particularly matrix metalloproteinases(MMPs)-released within the immune-infiltrated inflammatory microenvironment can remodel the ECM. Cytokines such as tumor necrosis factor-α (TN
View details →Ashley Rich, Ziqi Li, Alessandro De Simone et al. · 2026 · Nature Physics
Amputated salamander limbs or fish fins precisely regenerate to their pre-injury size, providing a paradigm for positional memory. Although this phenomenon has been appreciated for centuries, how position-dependent cues control tissue…
View details →Borbála Turcsán, Dorottya Júlia Ujfalussy, Andrea Kerepesi et al. · 2025 · Scientific Reports
Most dog owners in Western countries regard their dogs as companions or family members, prompting extensive research on the human-animal bond. However, many studies rely on broad categories or single dimensions, such as satisfaction or attachment, which can oversimplify the complexity of these relationships. In contrast, our study employed a multifaceted approach, examining the dog-owner bond across 13 relationship scales and comparing these scales to four different types of human relationships to reveal the human-like roles of dogs within a family. Participants (N = 717) characterized their r
View details →Victoria Bae‐Jump, Michael W. Sill, Paola A. Gehrig et al. · 2025 · Gynecologic Oncology
INTRODUCTION: We evaluated the efficacy of the addition of the anti-diabetic drug metformin to standard-of-care paclitaxel and carboplatin (PC) in patients with advanced and recurrent endometrial cancer (EC). METHODS: In this phase II/III…
View details →Mathilde Bourdon, Chloé Maignien, Marion Ouazana et al. · 2025 · Reproductive BioMedicine Online
Oestradiol plays a crucial role in reproduction, particularly in assisted reproductive technology (ART), where it can reach supraphysiological concentrations. These fluctuations occur during ovarian stimulation in fresh embryo transfer cycles and during endometrial preparation for frozen embryo transfer, potentially impacting implantation and perinatal outcomes. Oestradiol influences endometrial proliferation, receptivity, implantation and placentation, with the sensitivity of the endometrium to systemic oestrogen emerging as a key determinant of reproductive success. In fresh embryo transfer
View details →Richard E. Pratley, Douglas Scott Denham, Rupal Trivedi et al. · 2026 · Nature Medicine
Abstract Loss of lean mass in proportion to total weight loss is observed with incretin mimetic therapies such as tirzepatide and has the potential to adversely affect health and function. Apitegromab is an investigational, fully human monoclonal antibody that selectively inhibits myostatin activation and is, thereby, capable of increasing muscle mass. In the randomized, double-blind, placebo-controlled phase 2 EMBRAZE study, adults with overweight or obesity ( n = 102) were randomized 1:1 to receive tirzepatide plus apitegromab (10 mg kg −1 ) or tirzepatide plus placebo. At week 24, apitegrom
View details →Pegah Ataei, Hamidreza Kalantari, Tamara S. Bodnar et al. · 2026 · Frontiers in Microbiomes
The human gut microbiome has emerged as a pivotal modulator of brain function and mental health, acting through intricate bidirectional communication along the gut-brain axis. Mounting evidence suggests that microbial communities influence neurodevelopment, neurotransmission, and behavior via pathways involving the vagus nerve, immune signaling, and microbiota-derived metabolites such as short-chain fatty acids and neurotransmitter precursors. This review critically examines the mechanistic underpinnings of microbiota-brain communication and evaluates current findings linking dysbiosis to psyc
View details →Chengjiao Yang, Ruici Yang, Binbin Zheng et al. · 2026 · Cell Death and Disease
Protein lactylation emerges as a pivotal metabolic rheostat, translating microenvironmental lactate flux into stable programs that orchestrate cancer treatment resistance. This review synthesizes recent advances under the framework of "Lactylation Switch in Cancer Vulnerabilities." We dissect the dominant enzymatic pathways (AARS1/2, KATs, HDACs) and non-enzymatic mechanisms (MGO/LGSH), alongside their critical structural underpinnings. Furthermore, we delineate how lactylation signals are interpreted by specific readers (e.g., TRIM33), directly reprogram non-histone protein function through s
View details →Sukeshi Patel Arora, Laura Tenner, John Sarantopoulos et al. · 2019 · Journal of Clinical Oncology
3551 Background: Agents targeting the angiogenic pathway have been a cornerstone therapy in mCRC. In chemo-refractory mCRC, RGF, an oral multikinase inhibitor with considerable angiogenic inhibition, has shown modest effects on survival.…
View details →Gabriele Meyer, Jeffrey R. Leipprandt, Jianwei Xie et al. · 2012 · Endocrinology
Mammary organoids from adult mice produce tubules, analogous to mammary ducts in vivo, in response to hepatocyte growth factor (HGF) when cultured in collagen gels. The combination of HGF plus progestin (R5020) causes reduced tubule number…
View details →Jennifer Zou, Jinjing Zhou, Sarah Faller et al. · 2022 · G3 Genes Genomes Genetics
Genome-wide association studies (GWAS) have identified thousands of genetic variants associated with complex human traits, but only a fraction of variants identified in discovery studies achieve significance in replication studies. Replication in genome-wide association studies has been well-studied in the context of Winner's Curse, which is the inflation of effect size estimates for significant variants due to statistical chance. However, Winner's Curse is often not sufficient to explain lack of replication. Another reason why studies fail to replicate is that there are fundamental difference
View details →Jong Won Shin, Minh Nguyen Thien, Sun Ha Jee · 2025 · Healthcare
Background: Bilirubin is a potent endogenous antioxidant that plays a key role in regulating oxidative stress and inflammation, both closely linked to lung carcinogenesis. This study reinterprets the ALBI (Albumin–Bilirubin) and PALBI (Platelet–Albumin–Bilirubin) indices as composite markers of antioxidant and inflammatory status and evaluates their associations with lung cancer risk by sex, including stratified analyses by major lifestyle factors such as smoking and alcohol use. Methods: This study utilized data from the Korean Cancer Prevention Study-II (KCPS-II) cohort, which included 133,6
View details →Sami Alobaidi · 2025 · Frontiers in Medicine
Chronic kidney disease (CKD) remains a major global health issue, affecting millions and presenting persistent diagnostic and therapeutic challenges. Conventional biomarkers such as serum creatinine and estimated glomerular filtration rate have well-recognized limitations, underscoring the need for novel diagnostic tools and interventions. Emerging evidence highlights the gut-kidney axis as a central contributor to CKD pathogenesis, shaped by microbial dysbiosis and altered metabolite production. Harmful metabolites such as indoxyl sulfate, p-cresyl sulfate, and trimethylamine-N-oxide promote
View details →Giacomo Mutti, Eduard Ocaña‐Pallarès, Toni Gabaldón · 2025 · Molecular Biology and Evolution
Recent developments in protein structure prediction have allowed the use of this previously limited source of information at genome-wide scales. It has been proposed that the use of structural information may offer advantages over…
View details →Alicia Huerta‐Chagoya, Joohyun Kim, Ravi Mandla et al. · 2026 · The Lancet Diabetes & Endocrinology
BACKGROUND: Polygenic risk scores (PRSs) improve prediction of the development of type 2 diabetes over the use of clinical risk factors alone; however, they perform poorly in populations of non-European ancestry, limiting their global…
View details →Zhongwei Liu, Shuang Shi, Haitao Zhu et al. · 2018 · International Heart Journal
The phenotype shifting of vascular smooth muscle cells (VSMCs) was indicated to play a role during the initial stage of atherosclerotic plaque formation by facilitating extracellular matrix deposition. This study was aimed at investigating…
View details →Peipei Ma, Mogens Sandø Lund, Xiangdong Ding et al. · 2014 · Journal of Animal Breeding and Genetics
This study investigated the effect of including Nordic Holsteins in the reference population on the imputation accuracy and prediction accuracy for Chinese Holsteins. The data used in this study include 85 Chinese Holstein bulls genotyped…
View details →Gabriel Baldanzi, Anna Larsson, Sergi Sayols-Baixeras et al. · 2026 · Nature Medicine
Disruptions in gut microbiome are implicated in cardiometabolic disorders and other health outcomes. Antibiotics are known gut microbiome disruptors, but their long-term consequences remain underexplored. Here we combined individual-level data from the Swedish Prescribed Drug Register with fecal metagenomes of 14,979 adults to examine the association between oral antibiotic use over 8 years and gut microbiome. In multivariable confounder-adjusted regression models, antibiotic use <1 year before fecal sampling was associated with the greatest reduction in species diversity, but significant asso
View details →Jeanne M. McCaffery, George D. Papandonatos, Lucy F. Faulconbridge et al. · 2015 · Psychosomatic Medicine
OBJECTIVES: Numerous studies have found elevated depressive symptoms among individuals with Type 2 diabetes, yet the mechanisms remain unclear. We examined whether genetic loci previously associated with depressive symptoms predict…
View details →A. AbdAllah · 2026 · RSC Advances
In an attempt to collect clinical data about HDAC inhibitors as very significant anticancer drugs we aimed to compare data and reveal the impact of the structural features, concluding the points of interest that are likely to help further development of better cancer therapy. We presented results of different clinical phases of HDAC inhibitors classified as hydroxamic acid derivatives, cyclic peptides, benzamides, and short chain aliphatic acids in a coherent and cohesive manner. It was found that HDAC inhibitors are preferentially combined with other antitumor drugs, mainly anti PD-1 and doxo
View details →Nick Quinn-Bohmann, Alex V. Carr, Sean M. Gibbons · 2026 · PLoS Biology
Prebiotic, probiotic, and combined (synbiotic) interventions often show variable outcomes across individuals, driven by complex interactions between introduced biotics, the endogenous microbiota, and the host diet. Predicting individual-specific success or failure of probiotic and prebiotic therapies remains a major challenge. Here, we leverage microbial community-scale metabolic models (MCMMs) to predict probiotic engraftment and microbiota-mediated short-chain fatty acid (SCFA) production in response to probiotic and prebiotic interventions. Using data from two human clinical trial cohorts,
View details →Anagha Krishna, Alison Meynert, Karamjit Singh Dolt et al. · 2026 · Nature Genetics
CTNNB1, the gene encoding β-catenin, is a frequent target for oncogenic mutations activating the canonical Wnt signaling pathway, typically through missense mutations within a degron hotspot motif in exon 3. Here, we combine saturation genome editing with a fluorescent reporter assay to quantify signaling phenotypes for all 342 possible missense mutations in the mutation hotspot. Our data define the genetic requirements for β-catenin degron function, refine the consensus motif for substrate recognition by β-TRCP and reveal diverse levels of signal activation among known driver mutations. Tumor
View details →Lucia Scisciola, Manuela Giovanna Basilicata, Marta Belmonte et al. · 2026 · Nutrients
BACKGROUND: The heart depends on a continuous and flexible energy supply from fatty acids, glucose, and other substrates. Emerging evidence shows that gut microbiota-derived metabolites-such as trimethylamine-N-oxide (TMAO), short-chain fatty acids (SCFAs), secondary bile acids, indoles, phenylacetylglutamine (PAGln), and branched-chain amino acids-modulate cardiac metabolism and function. Although clinical evidence linking these metabolites to cardiovascular outcomes is expanding, most data remain associative, with limited causal or interventional proof. METHODS: A comprehensive narrative rev
View details →Yang Fu, Jie Liu, Zeming Mo et al. · 2026 · MedComm
ABSTRACT Melanoma is the most aggressive skin malignant tumor, typically exhibiting a high mutation burden and potentially harboring mutations in NRAS, BRAF, or NF1. To enhance survival rates, these driver alterations can achieve significant antitumor activity through targeted therapy. In the past decade, BRAF inhibitors combined with MEK inhibitors significantly improved the prognosis of BRAF mutation melanoma. Nevertheless, researchers have attempted various strategies to block the NRAS signaling pathway, NRAS mutation in melanoma is still considered to be untargetable. In recent years, MEK
View details →Sarah Ancheta, Leah C. Dorman, Guillaume Le Treut et al. · 2026 · PLoS Computational Biology
Single-cell RNA sequencing is revolutionizing our understanding of cell state dynamics, allowing researchers to capture and quantify the transcriptomic profile of a single cell at a specific timepoint. Among the computational techniques used to predict cellular trajectories, RNA velocity has emerged as a predominant tool for modeling transcriptional dynamics. RNA velocity leverages the mRNA maturation process to generate velocity vectors that predict the likely future state of a cell, offering insights into cellular differentiation, aging, and disease progression. Although this technique has s
View details →Jorinde van de Vis, Jordy de Vries, Marieke Postma · 2026 · Progress in Particle and Nuclear Physics
The origin of the universal asymmetry between matter and antimatter remains a mystery. Electroweak baryogenesis is a well-motivated mechanism for generating the asymmetry dynamically, using interesting features of the Standard Model. In addition, it relies on beyond-the-Standard Model physics active around the electroweak scale: new physics coupling to the Higgs to make the electroweak phase transition first order, and a new mechanism of CP violation. The relatively low energy scale at which electroweak baryogenesis occurs makes certain aspects of the mechanism testable through collider experi
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