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19,810 real negative results, null findings, and replication failures · Negative / Null Result Report. Search the index →

WASTE indexes published research — it does not host or republish full papers. Each entry is a metadata record compiled from open scholarly databases; the abstract is shown in full only where the paper is openly licensed, otherwise a short excerpt under fair use. Classifications are automated and approximate.

Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Invited Review: Inflammation and Health in the Transition Period Influence Reproductive Function in Dairy Cows

T.C. Bruinjé, S.J. LeBlanc · 2025 · Animals

In the early postpartum period, dairy cows undergo significant adaptations in Ca and lipid metabolism, immune function, and inflammatory processes. Concurrent exposure to endotoxins from the uterus, gastrointestinal tract, or mammary gland increases the risk of disease and reproductive problems. Metabolic and inflammatory imbalances during this phase can have both immediate and long-term effects on reproductive health. Associations between metabolic disorders and reproductive outcomes are often confounded by immune activation and systemic inflammation. However, optimal markers, thresholds, and

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Negative / Null Result ReportOpen accessNeuroscience

The role of plasma inflammatory markers in late-life depression and conversion to dementia: a 3-year follow-up study

Mariia Bocharova, Tom Borza, Leiv Otto Watne et al. · 2025 · Molecular Psychiatry

Late-life depression (LLD) has been linked to increased likelihood of dementia, although mechanisms responsible for this association remain largely unknown. One feature frequently observed in both LLD and dementia is elevated levels of plasma inflammatory markers. The present study aimed to compare the levels of 12 plasma inflammatory markers between older people with LLD and controls, and to explore whether these markers, along with clinical characteristics, can predict dementia in patients with LLD within 3 years of follow-up. Using multiple linear regression with stepwise adjustment, we com

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Negative / Null Result ReportOpen accessMedicine

Integrating Artificial Intelligence and PET Imaging for Drug Discovery: A Paradigm Shift in Immunotherapy

Jeremy McGale, Harrison J. Howell, Arnaud Beddok et al. · 2024 · Pharmaceuticals

The integration of artificial intelligence (AI) and positron emission tomography (PET) imaging has the potential to become a powerful tool in drug discovery. This review aims to provide an overview of the current state of research and highlight the potential for this alliance to advance pharmaceutical innovation by accelerating the development and deployment of novel therapeutics. We previously performed a scoping review of three databases (Embase, MEDLINE, and CENTRAL), identifying 87 studies published between 2018 and 2022 relevant to medical imaging (e.g., CT, PET, MRI), immunotherapy, arti

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Negative / Null Result ReportOpen accessEngineering

Experimental Investigation of the Bearing-Deformation Behavior of Broken Rocks in Goafs Under Various Influencing Factors

Yue Zhao, Jiang Su, Zhengzhen An et al. · 2026 · Applied Sciences

Coal gangue is one of the most abundant solid wastes generated during coal mining. The use of coal gangue for underground backfilling is widely recognized as an effective approach to reducing waste accumulation and promoting sustainable utilization. To further investigate the bearing and deformation behavior of underground gangue filling materials, combined with the underground occurrence conditions of crushed gangue in goaf, a self-designed loading apparatus for crushed gangue was employed to perform lateral compression experiments on crushed gangue. The compaction deformation, fractal dimens

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Negative / Null Result ReportOpen accessNeuroscience

Psychological mechanisms of offset analgesia: The effect of expectancy manipulation

Tibor M. Szikszay, Wacław M. Adamczyk, Janina Panskus et al. · 2023 · PLoS ONE

A frequently used paradigm to quantify endogenous pain modulation is offset analgesia, which is defined as a disproportionate large reduction in pain following a small decrease in a heat stimulus. The aim of this study was to determine whether suggestion influences the magnitude of offset analgesia in healthy participants. A total of 97 participants were randomized into three groups (hypoalgesic group, hyperalgesic group, control group). All participants received four heat stimuli (two constant trials and two offset trials) to the ventral, non-dominant forearm while they were asked to rate the

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Negative / Null Result ReportOpen accessMedicine

Replication of H9 influenza viruses in the human ex vivo respiratory tract, and the influence of neuraminidase on virus release

Renee W. Y. Chan, Louisa Chan, Chris Ka Pun Mok et al. · 2017 · Scientific Reports

H9N2 viruses are the most widespread influenza viruses in poultry in Asia. We evaluated the infection and tropism of human and avian H9 influenza virus in the human respiratory tract using ex vivo respiratory organ culture. H9 viruses infected the upper and lower respiratory tract and the majority of H9 viruses had a decreased ability to release virus from the bronchus rather than the lung. This may be attributed to a weak neuraminidase (NA) cleavage of carbon-6-linked sialic acid (Sia) rather than carbon-3-linked Sia. The modified cleavage of N-acetlylneuraminic acid (Neu5Ac) and N-glycolylne

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Negative / Null Result ReportOpen accessMedicine

Rescue of Infectious Rotavirus Reassortants by a Reverse Genetics System Is Restricted by the Receptor-Binding Region of VP4

Alexander Falkenhagen, Marno Huyzers, Alberdina A. van Dijk et al. · 2021 · Viruses

The rotavirus species A (RVA) capsid contains the spike protein VP4, which interacts with VP6 and VP7 and is involved in cellular receptor binding. The capsid encloses the genome consisting of eleven dsRNA segments. Reassortment events can result in novel strains with changed properties. Using a plasmid-based reverse genetics system based on simian RVA strain SA11, we previously showed that the rescue of viable reassortants containing a heterologous VP4-encoding genome segment was strain-dependent. In order to unravel the reasons for the reassortment restrictions, we designed here a series of

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Negative / Null Result ReportOpen accessImmunology and Microbiology

Uracil DNA glycosylase interacts with the p32 subunit of the replication protein A complex to modulate HIV-1 reverse transcription for optimal virus dissemination

Cécile Hérate, Clarisse Vigne, Carolin A. Guenzel et al. · 2016 · Retrovirology

BACKGROUND: Through incorporation into virus particles, the HIV-1 Vpr protein participates in the early steps of the virus life cycle by influencing the reverse transcription process. We previously showed that this positive impact on reverse transcription was related to Vpr binding to the uracil DNA glycosylase 2 enzyme (UNG2), leading to enhancement of virus infectivity in established CD4-positive cell lines via a nonenzymatic mechanism. RESULTS: We report here that Vpr can form a trimolecular complex with UNG2 and the p32 subunit (RPA32) of the replication protein A (RPA) complex and we expl

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Negative / Null Result ReportOpen accessMedicine

Underestimating Calorie Content When Healthy Foods Are Present: An Averaging Effect or a Reference-Dependent Anchoring Effect?

Suzanna Forwood, Amy L. Ahern, Gareth J Hollands et al. · 2013 · PLoS ONE

OBJECTIVE: Previous studies have shown that estimations of the calorie content of an unhealthy main meal food tend to be lower when the food is shown alongside a healthy item (e.g. fruit or vegetables) than when shown alone. This effect has been called the negative calorie illusion and has been attributed to averaging the unhealthy (vice) and healthy (virtue) foods leading to increased perceived healthiness and reduced calorie estimates. The current study aimed to replicate and extend these findings to test the hypothesized mediating effect of ratings of healthiness of foods on calorie estimat

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Negative / Null Result ReportMedicine

SAKK 24/09: Safety and tolerability of bevacizumab plus paclitaxel versus bevacizumab plus metronomic cyclophosphamide and capecitabine as first-line therapy in patients with HER2-negative advanced stage breast cancer—A multicenter, randomized phase III trial.

Christoph Rochlitz, Roger von Moos, Martin Bigler et al. · 2014 · Journal of Clinical Oncology

518 Background: Bevacizumab combined with chemotherapy has been shown to improve response rate and PFS in metastatic breast cancer. The aim of our study was to demonstrate decreased toxicity of metronomic chemotherapy/bevacizumab when…

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Negative / Null Result ReportImmunology and Microbiology

Deficiency of ULK1/ATG1 in the follicle cells disturbs ER homeostasis and causes defective chorion deposition in the vector Rhodnius prolixus

Larissa Bomfim, Isabela Ramos · 2020 · The FASEB Journal

In insects, synthesis and deposition of the chorion (eggshell) are performed by the professional secretory follicle cells (FCs) that surround the oocytes in the course of oogenesis. Here, we found that ULK1/ATG1, an autophagy-related…

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Negative / Null Result ReportOpen accessImmunology and Microbiology

Detection and characterization of diverse coccidian protozoa shed by California sea lions

Yvette A. Girard, Christine K. Johnson, Heather Fritz et al. · 2015 · International Journal for Parasitology Parasites and Wildlife

Tissue-cyst forming coccidia in the family Sarcocystidae are etiologic agents of protozoal encephalitis in marine mammals including the federally listed Southern sea otter (Enhydra lutris). California sea lions (Zalophus californianus),…

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Negative / Null Result ReportOpen accessPsychology

Characteristics of personally important episodic memories, counterfactual thoughts, and future projections across age and culture

Müge Özbek, Annette Bohn, Dorthe Berntsen · 2020 · Applied Cognitive Psychology

Summary We have limited knowledge as to whether the phenomenological differences between episodic memories, counterfactuals, and future projections show the same pattern across age groups and diverse samples. Here we compared the…

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Negative / Null Result ReportBusiness, Management and Accounting

THE EFFECTS OF PERCEIVED SERVICE QUALITY ON PATIENT SATISFACTION AT A PUBLIC HOSPITAL IN STATE OF PAHANG, MALAYSIA

Noor Azlinna Azizan, Bahari Mohamed · 2013 · Asian journal of social sciences & humanities

This study focuses on the effects of perceived service quality on patient satisfaction in a public hospital. Data was collected from 109 respondents that experienced the hospital service. Using a PLS-SEM tool, the hypothesized effects…

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Negative / Null Result ReportSocial Sciences

Career Indecision in China

Meghan Roche, Andrea Carr, In Heok Lee et al. · 2016 · Journal of Career Assessment

This study explored the measurement equivalence of the Career Indecision Profile (CIP) in a Chinese sample with both U.S. and South Korean samples. Past measurement invariance research on the CIP in four international samples (Icelandic…

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Comparing qPCR and DNA methylation-based measurements of telomere length in a high-risk pediatric cohort

Waylon J. Hastings, Laura Etzel, Christine M. Heim et al. · 2022 · Aging

Various approaches exist to assess population differences in biological aging. Telomere length (TL) is one such measure, and is associated with disease, disability and early mortality. Yet, issues surrounding precision and reproducibility are a concern for TL measurement. An alternative method to estimate TL using DNA methylation (DNAmTL) was recently developed. Although DNAmTL has been characterized in adult and elderly cohorts, its utility in pediatric populations remains unknown. We examined the comparability of leukocyte TL measurements generated using qPCR (absolute TL; aTL) to those esti

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Negative / Null Result ReportOpen accessEnvironmental Science

Sub-lethal effects of energy development on a migratory mammal—The enigma of North American pronghorn

Jon P. Beckmann, Sarah H. Olson, Renee G. Seidler et al. · 2016 · Global Ecology and Conservation

To meet societal demands for energy, ∼50,000 gas sites are developed annually in North America, among which many are in western less-developed and wildlife rich areas. To evaluate effects of increasing energy infrastructure requires…

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Negative / Null Result ReportMedicine

Factors and consequences of hypertension

Alberto Zanchetti · 2016 · Journal of Hypertension

The articles published in the current issue of the Journal of Hypertension focus on risk factors for the development of hypertension and on the relationship of hypertension with cardiovascular disease. Two articles illustrate the role of…

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Negative / Null Result ReportNeuroscience

On the usefulness of formal judgment tasks in syntax and in second-language research: The case of resumptive pronouns in English, Turkish, and Mandarin Chinese

John Hitz, Elaine J. Francis · 2016 · Linguistics

Abstract Gibson and Fedorenko (2013, The need for quantitative methods in syntax and semantics research, Language and Cognitive Processes 28(2), 88–124) have argued against the continued use of informally collected acceptability judgments…

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Negative / Null Result ReportOpen accessNeuroscience

Single nucleotide polymorphism rs6716901 in SLC25A12 gene is associated with Asperger syndrome

Jaroslava Durdiaková, Varun Warrier, Simon Baron‐Cohen et al. · 2014 · Molecular Autism

BACKGROUND: Autism Spectrum Conditions (ASC) are a group of developmental conditions which affect communication, social interactions and behaviour. Mitochondrial oxidative dysfunction has been suggested as a mechanism of autism based on the results of multiple genetic association and expression studies. SLC25A12 is a gene encoding a calcium-binding carrier protein that localizes to the mitochondria and is involved in the exchange of aspartate for glutamate in the inner membrane of the mitochondria regulating the cytosolic redox state. rs2056202 SNP in this gene has previously been associated w

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Artificial Intelligence for Exosomal Biomarker Discovery for Cardiovascular Diseases: Multi-Omics Integration, Reproducibility, and Translational Prospects

Rasit Dinc, Nurittin Ardıç · 2026 · Cells

Exosomes and other extracellular vesicles (EVs) carry microRNAs, proteins, and lipids that reflect cardiovascular pathophysiology and can enable minimally invasive biomarker discovery. However, EV datasets are highly dimensional and heterogeneous, strongly influenced by pre-analytic variables and non-standardized isolation/characterization workflows, limiting reproducibility across studies. Artificial intelligence (AI), including machine learning (ML), deep learning (DL), and network-based approaches, can support EV biomarker development by integrating multi-omics profiles with clinical metada

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Negative / Null Result ReportOpen accessSocial Sciences

The “Wallpaper Effect” Revisited: Divergent Findings on the Effects of Intergroup Contact on Attitudes in Diverse Versus Nondiverse Contexts

Katharina Schmid, Ralf Wölfer, Herman Swart et al. · 2017 · Personality and Social Psychology Bulletin

This article reexamines the so-called "wallpaper effect" of intergroup contact, which contends that for minority group members living in areas more densely populated by majority group members, intergroup contact fails to reduce prejudice. We tested this claim in five studies, using data from five countries, two types of contexts, a range of measures, and involving different minority versus majority groups. Using multilevel cross-level interaction models, we considered whether effects of contact on outgroup attitudes were moderated by relative outgroup size. Results failed to replicate the prev

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Negative / Null Result ReportOpen accessEconomics, Econometrics and Finance

Economic Growth, FDI, Tourism, and Agricultural Productivity as Drivers of Environmental Degradation: Testing the EKC Hypothesis in ASEAN Countries

Yuldoshboy Sobirov, Beruniy Artikov, Elbek Khodjaniyazov et al. · 2025 · Sustainability

This study examines the long-run relationship between carbon dioxide (CO2) emissions and key macroeconomic and sectoral drivers in ten ASEAN economies from 1995 to 2023. Employing Driscoll–Kraay standard errors, Prais–Winsten regression, heteroskedastic panel-corrected standard errors, Fully Modified Ordinary Least Squares (FMOLS), Dynamic Ordinary Least Squares (DOLS), and Canonical Cointegrating Regression (CCR) estimators, the analysis accounts for cross-sectional dependence, slope heterogeneity, and endogeneity. Results indicate that GDP exerts a more-than-unitary positive effect on emissi

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