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136 real negative results, null findings, and replication failures in Agricultural and Biological Sciences · Negative / Null Result Report. Search the index →

WASTE indexes published research — it does not host or republish full papers. Each entry is a metadata record compiled from open scholarly databases; the abstract is shown in full only where the paper is openly licensed, otherwise a short excerpt under fair use. Classifications are automated and approximate.

Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Temperature-dependent metabolic consequences of food deprivation in the European sardine

Elisa Thoral, Damien Roussel, Éric Gasset et al. · 2023 · Journal of Experimental Biology

Aquatic ecosystems can exhibit seasonal variation in resource availability and animals have evolved to cope with the associated caloric restriction. During winter in the NW Mediterranean Sea, the European sardine Sardina pilchardus…

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Eristalis flower flies can be mechanical vectors of the common trypanosome bee parasite, Crithidia bombi

Abby E. Davis, Kaitlin R. Deutsch, Alondra M. Torres et al. · 2021 · Scientific Reports

Flowers can be transmission platforms for parasites that impact bee health, yet bees share floral resources with other pollinator taxa, such as flies, that may be hosts or non-host vectors (i.e., mechanical vectors) of parasites. Here, we assessed whether the fecal-orally transmitted gut parasite of bees, Crithidia bombi, can infect Eristalis tenax flower flies. We also investigated the potential for two confirmed solitary bee hosts of C. bombi, Osmia lignaria and Megachile rotundata, as well as two flower fly species, Eristalis arbustorum and E. tenax, to transmit the parasite at flowers. We

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Negative / Null Result ReportAgricultural and Biological Sciences

Stay cool, travel far: cold‐acclimated oriental fruit moth females have enhanced flight performance but lay fewer eggs

Aurélie Ferrer, Dominique Mazzi, Silvia Dorn · 2014 · Entomologia Experimentalis et Applicata

Abstract Acclimation to a particular environment may provide organisms with advantages in that environment. In species with multiple generations per year, acclimation may cause life‐history traits to vary with season and between…

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Negative / Null Result ReportAgricultural and Biological Sciences

A genome‐wide significant association on chromosome 2 for footrot resistance/susceptibility in Swiss White Alpine sheep

Allegra Niggeler, Jens Tetens, Anna Stäuble et al. · 2017 · Animal Genetics

Footrot is one of the most important causes of lameness in global sheep populations and is characterized by a bacterial infection of the interdigital skin. As a multifactorial disease, its clinical representation depends not only on…

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Negative / Null Result ReportAgricultural and Biological Sciences

Cortical Neurons Derived from Equine Induced Pluripotent Stem Cells Are Susceptible to Neurotropic Flavivirus Infection and Replication: An In Vitro Model for Equine Neuropathic Diseases

Patrick R.J. Fortuna, Helle Bielefeldt‐Ohmann, Dmitry A. Ovchinnikov et al. · 2018 · Stem Cells and Development

Horses are susceptible to a number of neurotropic viruses, including West Nile virus (WNV), which is a pathogen of global significance in both horses and humans. However, there are no in vitro models with which to study infectious…

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Response of broilers to supplementation of branched-chain amino acids blends with different valine contents in the starter period under summer conditions

Canan Kop Bozbay, Ahmet Akdag, Helin Atan et al. · 2020 · Animal Bioscience

OBJECTIVE: The objectives of this study were to compare the effects of normal and low protein content (PC) of starter diet supplemented or not with blends of branched-chain amino acids (BCAAs) on growth performance of broilers under summer conditions and to investigate whether these effects altered some quality traits and the characteristics of gastrointestinal tract. METHODS: A total of 768 mixed-sex broiler chicks (Ross 308, one-d-old) with an average initial body weight (BW) of 47.6±1.03 g were allocated into six treatments with four replications in 2×3 factorial arrangement. Factors were:

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Effects of adding an automated monitoring device to the health screening of postpartum Holstein cows on survival and productive and reproductive performances

Manuel A. Silva, Anderson Veronese, Anna Luiza Belli et al. · 2021 · Journal of Dairy Science

kg) than cows diagnosed with a clinical disease that had an AMD alert. Adding AMD-generated health alerts to the health screening of postpartum cows in a herd with an existing screening program did not improve survival, milk yield, or…

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Negative / Null Result ReportAgricultural and Biological Sciences

Benefits of larval group feeding by Chrysolina aurichalcea asclepiadis on Vincetoxicum: improved host location or feeding facilitation?

Aaron S. Weed · 2010 · Entomologia Experimentalis et Applicata

Abstract Several studies have shown that group feeding by immature insects enhances larval performance through feeding facilitation. However, the benefits of this behavior have not been studied for species that hatch off from their host…

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Invited Review: Inflammation and Health in the Transition Period Influence Reproductive Function in Dairy Cows

T.C. Bruinjé, S.J. LeBlanc · 2025 · Animals

In the early postpartum period, dairy cows undergo significant adaptations in Ca and lipid metabolism, immune function, and inflammatory processes. Concurrent exposure to endotoxins from the uterus, gastrointestinal tract, or mammary gland increases the risk of disease and reproductive problems. Metabolic and inflammatory imbalances during this phase can have both immediate and long-term effects on reproductive health. Associations between metabolic disorders and reproductive outcomes are often confounded by immune activation and systemic inflammation. However, optimal markers, thresholds, and

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Implications of root morphology and anatomy for water deficit tolerance and recovery of grapevine rootstocks

David García Alonso, Ignacio Buesa, Luis Flor et al. · 2025 · Frontiers in Plant Science

The intensification of drought conditions due to climate change poses a major challenge to sustainable grape production. Rootstocks are essential in supporting grapevine water uptake and drought resilience; however, their physiological responses to water stress are not fully understood. Under the hypothesis that root morphology and anatomy may be key traits in grapevine tolerance to water deficit, this study aimed to investigate these traits across diverse rootstocks under progressive water deficit and recovery phases. Thirteen genotypes, including commercial rootstocks and recently bred RG-se

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Functional implications of residues of the B′ motif of geminivirus replication initiator protein in its helicase activity

Rajrani Ruhel, Mohit Mazumder, Prabu Gnanasekaran et al. · 2021 · FEBS Journal

Geminivirus replication initiator protein (Rep) is a multifunctional viral protein required for replication. During the process of viral replication, Rep acts as a site- and strand-specific endonuclease, ligase, ATPase, and helicase. B'…

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Diversity of adapted tobacco microbial community and its application in improving tobacco quality

Jing Mai, Ying Ning, Zhonglong Lin et al. · 2025 · Industrial Crops and Products

Many bacteria such as Bacillus , Pseudomonas , and Lactobacillus are widely present in flue-cured tobacco leaves during the aging process, indicating they play important roles in improving tobacco quality. In this study, we aimed to screen…

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Unravelling Changes in the Pinus radiata Root and Soil Microbiomes as a Function of Aridity

Sarah L. Addison, Zhenzhen Yan, Thomas F. Carlin et al. · 2025 · Global Change Biology

Increased aridity is emerging as a key impact of climate change in terrestrial ecosystems globally. Forest biomes are particularly vulnerable to the impacts of changing environmental conditions due to their long-lived and sessile nature.…

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Mechanism, Efficacy, and Safety of Natural Antibiotics

Andrei Teodor Matei, Anita Ioana Vișan · 2025 · Antibiotics

The growing ineffectiveness of common antibiotics against multidrug-resistant pathogens has made antimicrobial resistance (AMR) a serious global health concern. This review emphasizes that natural antibiotics from animals, bacteria, fungi, and plants are worthy alternatives for combating this crisis. Evolutionary pressure has shaped these molecules, leading to antibiotic-resistant bacteria that can withstand single-target synthetic drugs but are vulnerable to multiple attack pathways (e.g., cell wall disruption, protein synthesis inhibition, biofilm interference) from natural compounds. Natura

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Anticoccidial and immunogenic effectivity of encapsulated organic acids and anticoccidial drugs in broilers infected with Eimeria spp.

Ali Nouri · 2022 · Scientific Reports

Abstract The study was conducted to consider the anticoccidial and immunogenic effectivities of encapsulated organic acids and anticoccidial drugs in broilers reared on a reused litter infected with Eimeria spp. for simulating in-field exposure to avian coccidiosis. 525 mixed -sex one-day-old broiler chicks (Ross 308) were used in a 2 × 3 factorial experiment as a completely randomized design with seven experimental groups and five replicates of 15 chicks. The seven experimental groups were included: negative (uninfected; T 1 ) and positive (infected; T 2 ) control groups fed a diet without ad

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Large-scale multi-omics unveils host–microbiome interactions driving root development and nitrogen acquisition

Nannan Li, Guoliang Li, Xiaofang Huang et al. · 2026 · Nature Plants

The rhizosphere microbiome plays a crucial role in determining plant performance and fitness. Nevertheless, regulatory mechanisms linking host genetic variation, root gene regulation and microbiome assembly-and their collective influence on plant nutritional traits-remain poorly understood. Here we generated and integrated 1,341 paired datasets, including root transcriptomes, rhizosphere bacterial 16S rRNA profiles and root ionomes, across 175 resequenced Brassica napus ecotypes grown at two contrasting field sites. We identified 203 highly heritable bacterial amplicon sequence variants (ASVs)

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Harnessing genotype-by-environment interaction to determine adaptability of advanced cowpea lines to multiple environments in Uganda

Abiriga Francis, O. Ongom Patrick, Rubaihayo Patrick et al. · 2020 · Journal of Plant Breeding and Crop Science

This study was conducted to determine the yield stability of advanced cowpea lines in diverse agro-ecological zones of Uganda in order to facilitate documentation requirements for national performance trials (NPT). Thirty cowpea genotypes were evaluated against six checks in three localities, over three growing seasons, making a total of 9 unique environments. The trials were laid in a 6x6 alpha lattice design with three replications and grain yield was the principal trait measured. Single-site and multi-location data were summarized using analysis of variance. Further analysis of stability wa

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Influence of soil properties for improving productivity and grain quality of rainfed rice

Chitra Priyadarshani, Ranjan Laik, Sumit Sow et al. · 2025 · Archives of Agronomy and Soil Science

Integrated crop residue management with micronutrient application can effectively addresses dual challenges of maintaining productivity while enhancing grain nutritional quality of rice in calcareous soils. This study evaluated the role of different soil properties for enhancing yield and grain quality of rice under 29 years of long-term effects of crop residue incorporation and zinc (Zn) fertilization in calcareous Typic Haplaquepts soils. Results showed 100% crop residue incorporation significantly enhanced soil organic carbon (SOC), available nitrogen, available Zn, soil respiration, and we

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

A novel strategy to attenuate porcine reproductive and respiratory syndrome virus by inhibiting viral replication in the target pulmonary alveolar macrophages via hematopoietic-specific miR-142

Shaoyuan Tan, Weixin Wu, Xinna Ge et al. · 2023 · One Health Advances

Porcine reproductive and respiratory syndrome virus (PRRSV) is an economically important pathogen for the global pork industry. Although modified live virus (MLV) vaccines are commonly used for PRRSV prevention and control, they still carry a risk of infecting the host and replicating in target cells, thereby increasing the likehood of virus recombination and reversion to virulence. In this study, we inserted the target sequence of miR-142 into the nsp2 hypervariable region of PRRSV to inhibit viral replication in its host cells of pigs, with the aim of achieving virus attenuation. The chimeri

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Persistence and Retention of Porcine Reproductive and Respiratory Syndrome Virus in Stable Flies (Diptera: Muscidae)

Kateryn Rochon, Rodney B. Baker, Glen W. Almond et al. · 2015 · Journal of Medical Entomology

We investigated the acquisition of porcine reproductive and respiratory syndrome (PRRS) virus by the stable fly (Diptera: Muscidae; Stomoxys calcitrans (L.)) through a bloodmeal, and virus persistence in the digestive organs of the fly…

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

The copepod Acartia tonsa as live feed for fat snook (Centropomus parallelus) larvae from notochord flexion to advanced metamorphosis

Márcia Vanacor Barroso, Cristina Vaz Avelar de Carvalho, Rosemar Antoniassi et al. · 2017 · Latin American Journal of Aquatic Research

From early development until the completion of metamorphosis, fat snook (Centropomus parallelus) larvae are commonly fed the rotifers Brachionus spp. and Artemia spp. nauplii. In this study, cultivated copepods Acartia tonsa were evaluated…

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

The beneficial effect of probiotics in the prevention of irinotecan-induced diarrhea in colorectal cancer patients with colostomy: a pooled analysis of two probiotic trials (Probio-SK-003 and Probio-SK-005) led by Slovak Cooperative Oncology Group

Michal Mego, Barbora Judita Kasperová, Jozef Chovanec et al. · 2024 · Frontiers in Oncology

Background: Probiotics could decrease irinotecan-induced diarrhea due to the reduction of intestinal beta-d-glucuronidase activity. This study included a combined analysis of two clinical trials aimed to determine the effectiveness of the probiotics in the prophylaxis of irinotecan-induced diarrhea in metastatic colorectal cancer (CRC) patients. Methods: This combined analysis included 46 patients with CRC enrolled in the Probio-SK-003 (NCT01410955) and 233 patients from Probio-SK-005 (NCT02819960) starting a new line of irinotecan-based therapy with identical eligibility criteria. Patients we

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Genomic and phenomic prediction for soybean seed yield, protein, and oil

Liza Van der Laan, Kyle Parmley, Mojdeh Saadati et al. · 2025 · The Plant Genome

Developments in genomics and phenomics have provided valuable tools for use in cultivar development. Genomic prediction (GP) has been used in commercial soybean [Glycine max L. (Merr.)] breeding programs to predict grain yield and seed…

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Integrative Genomics and Precision Breeding for Stress-Resilient Cotton: Recent Advances and Prospects

Zahra Ghorbanzadeh, Bahman Panahi, Leila Purhang et al. · 2025 · Agronomy

Developing climate-resilient and high-quality cotton cultivars remains an urgent challenge, as the key target traits yield, fibre properties, and stress tolerance are highly polygenic and strongly influenced by genotype–environment interactions. Recent advances in chromosome-scale genome assemblies, pan-genomics, and haplotype-resolved resequencing have greatly enhanced the capacity to identify causal variants and recover non-reference alleles linked to fibre development and environmental adaptation. Parallel progress in functional genomics and precision genome editing, particularly CRISPR/Cas

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Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Trichoderma in Sustainable Agriculture and the Challenges Related to Its Effectiveness

Karina Gutiérrez-Moreno, Ana Isabel Olguín-Martínez, Amelia C. Montoya-Martínez et al. · 2025 · Diversity

Fungi from the genus Trichoderma have been extensively studied and used as biological control agents (BCAs) because of their versatile mechanisms of action. These include triggering systemic resistance, directly inhibiting pathogens, promoting plant growth, enhancing tolerance to abiotic stress, and producing auxins. However, the widespread application of the most studied Trichoderma strains has been limited by discrepancies between their potential results observed in controlled environments and the outcomes in greenhouses and field conditions. These differences are associated with context dep

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