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Negative / Null Result ReportOpen accessNeoplasms. Tumors. Oncology. Including cancer and carcinogens

ERα and ERK1/2 MAP kinase expression in microdissected stromal and epithelial endometrial cells

Said Abu Alkhair Mohamed; Ihab Shafek Atta; Brian G. Rowan; Mohamed Mokhtar Desouki · 2014 · Journal of the Egyptian National Cancer Institute

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Abstract

Our previous published data detected higher expression of total and active mitogen activated protein kinase (MAPK) in the epithelial vs. stromal cells of the endometrium. In the present work we compared the expression of ERK1/2 MAPK and estrogen receptor α (ERα) in epithelial versus stromal cells in benign human endometrial tissues. Laser capture microdissection was used to separate glandular epithelium and stromal cells from six frozen, proliferative phase endometrial specimens. Total and phosphorylated levels for ERK1/2 and ERα were measured by quantitation of signals from Western blots usin

Abstract by Said Abu Alkhair Mohamed; Ihab Shafek Atta; Brian G. Rowan; Mohamed Mokhtar Desouki, Journal of the Egyptian National Cancer Institute (2014) — licensed CC BY 4.0.

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Metadata source: DOAJ · DOI 10.1016/j.jnci.2013.09.001