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8 real negative results, null findings, and replication failures in Biochemistry, Genetics and Molecular Biology · Failed Experiment Report. Search the index →

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Failed Experiment ReportOpen accessBiochemistry, Genetics and Molecular Biology

The Two-pore channel (TPC) interactome unmasks isoform-specific roles for TPCs in endolysosomal morphology and cell pigmentation

Yaping Lin-Moshier, Michael V. Keebler, Robert Hooper et al. · 2014 · Proceedings of the National Academy of Sciences

The two-pore channels (TPC1 and TPC2) belong to an ancient family of intracellular ion channels expressed in the endolysosomal system. Little is known about how regulatory inputs converge to modulate TPC activity, and proposed activation…

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Failed Experiment ReportOpen accessBiochemistry, Genetics and Molecular Biology

Genetic effects influencing risk for major depressive disorder in China and Europe

Tim B. Bigdeli, Stephan Ripke, Roseann E. Peterson et al. · 2017 · Translational Psychiatry

Abstract Major depressive disorder (MDD) is a common, complex psychiatric disorder and a leading cause of disability worldwide. Despite twin studies indicating its modest heritability (~30–40%), extensive heterogeneity and a complex genetic architecture have complicated efforts to detect associated genetic risk variants. We combined single-nucleotide polymorphism (SNP) summary statistics from the CONVERGE and PGC studies of MDD, representing 10 502 Chinese (5282 cases and 5220 controls) and 18 663 European (9447 cases and 9215 controls) subjects. We determined the fraction of SNPs displaying c

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Failed Experiment ReportOpen accessBiochemistry, Genetics and Molecular Biology

Phase 1/2 AAV5-hRKp.RPGR (Botaretigene Sparoparvovec) Gene Therapy: Safety and Efficacy in RPGR-Associated X-Linked Retinitis Pigmentosa

Michel Michaelides, Cagri G. Besirli, Yesa Yang et al. · 2024 · American Journal of Ophthalmology

PURPOSE: To assess the safety and efficacy of AAV5-hRKp.RPGR in participants with retinitis pigmentosa GTPase regulator (RPGR)-associated X-linked retinitis pigmentosa (XLRP). DESIGN: Open-label, phase 1/2 dose escalation/expansion study (ClinicalTrials.gov Identifier: NCT03252847). METHODS: vg/ml) was administered to the poorer-seeing eye (n = 10). Dose confirmation (intermediate dose) was carried out in 3 pediatric participants. In the dose expansion phase, 36 participants were randomized 1:1:1 to immediate (low or intermediate dose) or deferred (control) treatment. The primary outcome was s

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Failed Experiment ReportOpen accessBiochemistry, Genetics and Molecular Biology

Degradation of Rural and Urban Great Tit Song: Testing Transmission Efficiency

Emily J. Mockford, Rupert C. Marshall, Torben Dabelsteen · 2011 · PLoS ONE

Acoustic signals play a fundamental role in avian territory defence and mate attraction. Several studies have now shown that spectral properties of bird song differ between urban and rural environments. Previously this has been attributed to competition for acoustic space as a result of low-frequency noise present in cities. However, the physical structure of urban areas may have a contributory effect. Here we investigate the sound degradation properties of woodland and city environments using both urban and rural great tit song. We show that although urban surroundings caused significantly le

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Failed Experiment ReportOpen accessBiochemistry, Genetics and Molecular Biology

Prostate-Specific Antigen-Retargeted Recombinant Newcastle Disease Virus for Prostate Cancer Virotherapy

Shobana Raghunath, Siba K. Samal, Subbiah Elankumaran · 2013 · Journal of Virology

Oncolytic virus (OV) therapies of cancer are based on the use of replication-competent, tumor-selective viruses with limited toxicity. Newcastle disease virus (NDV), an avian paramyxovirus, is a promising OV and is inherently tumor…

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Failed Experiment ReportOpen accessBiochemistry, Genetics and Molecular Biology

Inhibitory Receptors Induced by VSV Viroimmunotherapy Are Not Necessarily Targets for Improving Treatment Efficacy

Kevin G. Shim, Shane Zaidi, Jill Thompson et al. · 2017 · Molecular Therapy

Systemic viroimmunotherapy activates endogenous innate and adaptive immune responses against both viral and tumor antigens. We have shown that therapy with vesicular stomatitis virus (VSV) engineered to express a tumor-associated antigen…

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Failed Experiment ReportOpen accessBiochemistry, Genetics and Molecular Biology

Genetic improvement of indigenous Greek sheep and goat breeds

Angeliki Argyriadou, Athanasios Ι. Gelasakis, Georgios Banos et al. · 2020 · Journal of the Hellenic Veterinary Medical Society

The objective of the study was twofold: (i) to review the genetics of production and resilience traits of indigenous Greek small ruminant breeds as well as the evolution of national breeding programs, and (ii) explore innovative and…

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Failed Experiment ReportOpen accessBiochemistry, Genetics and Molecular Biology

A Francisella tularensis Live Vaccine Strain That Improves Stimulation of Antigen-Presenting Cells Does Not Enhance Vaccine Efficacy

Deanna Schmitt, Dawn M. O’Dee, Joseph Horzempa et al. · 2012 · PLoS ONE

Vaccination is a proven strategy to mitigate morbidity and mortality of infectious diseases. The methodology of identifying and testing new vaccine candidates could be improved with rational design and in vitro testing prior to animal experimentation. The tularemia vaccine, Francisella tularensis live vaccine strain (LVS), does not elicit complete protection against lethal challenge with a virulent type A Francisella strain. One factor that may contribute to this poor performance is limited stimulation of antigen-presenting cells. In this study, we examined whether the interaction of genetical

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