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397 real negative results, null findings, and replication failures in Biochemistry, Genetics and Molecular Biology · Negative / Null Result Report. Search the index →

WASTE indexes published research — it does not host or republish full papers. Each entry is a metadata record compiled from open scholarly databases; the abstract is shown in full only where the paper is openly licensed, otherwise a short excerpt under fair use. Classifications are automated and approximate.

Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Blood-based extracellular matrix biomarkers as predictors of survival in patients with metastatic pancreatic ductal adenocarcinoma receiving pegvorhyaluronidase alfa

Song Wang, Cecilie L. Bager, M.A. Karsdal et al. · 2021 · Journal of Translational Medicine

BACKGROUND: Extensive extracellular matrix (ECM) remodeling is a hallmark of metastatic pancreatic ductal adenocarcinoma (mPDA). We investigated fragments of collagen types III (C3M, PRO-C3), VI (PRO-C6), and VIII (C8-C), and versican (VCANM) in plasma as biomarkers for predicting progression-free survival (PFS) and overall survival (OS) in patients with mPDA treated with pegvorhyaluronidase alfa, a biologic that degrades the ECM component hyaluronan (HA), in a randomized phase 2 study (HALO109-202). METHODS: HALO109-202 comprised a discovery cohort (Stage 1, n = 94) and a validation cohort (S

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Cardiometabolic phenotypes and mitochondrial DNA copy number in two cohorts of UK women

Anna L. Guyatt, Kimberley Burrows, Philip A. I. Guthrie et al. · 2017 · Mitochondrion

The mitochondrial genome is present at variable copy number between individuals. Mitochondria are vulnerable to oxidative stress, and their dysfunction may be associated with cardiovascular disease. The association of mitochondrial DNA copy number with cardiometabolic risk factors (lipids, glycaemic traits, inflammatory markers, anthropometry and blood pressure) was assessed in two independent cohorts of European origin women, one in whom outcomes were measured at mean (SD) age 30 (4.3) years (N=2278) and the second at 69.4 (5.5) years (N=2872). Mitochondrial DNA copy number was assayed by qua

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Negative / Null Result ReportBiochemistry, Genetics and Molecular Biology

Modelling vocal anatomy's significant effect on speech

Bart de Boer · 2010 · Journal of Evolutionary Psychology

This paper investigates the effect of larynx position on the articulatory abilities of a human-like vocal tract. Previous work has investigated models that were built to resemble the anatomy of existing species or fossil ancestors. This…

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Exosome secretion kinetics are controlled by temperature

Anarkali Mahmood, Zdeněk Otruba, Alan W. Weisgerber et al. · 2023 · Biophysical Journal

When multivesicular endosomes (MVEs) fuse with the plasma membrane, exosomes are released into the extracellular space where they can affect other cells. The ability of exosomes to regulate cells nearby or further away depends on whether…

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Chronic hyperglycemia and cardiovascular dysfunction: an in-depth exploration of metabolic and cellular pathways in type 2 diabetes mellitus

Araiz Hussain · 2025 · Cardiovascular Diabetology – Endocrinology Reports

Chronic hyperglycemia is the defining feature of type 2 diabetes mellitus (T2DM) and a central driver of its long-term complications, including microvascular and macrovascular diseases. Among these, cardiovascular disease (CVD) remains the leading cause of morbidity and mortality in individuals with T2DM. Persistent glucose elevation activates multiple interrelated biochemical pathways, including the polyol pathway, formation of advanced glycation end-products, protein kinase C activation, oxidative stress via several cellular mechanisms, as well as the hexosamine biosynthetic pathway. These p

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Propofol‑induced miR‑125a‑5p inhibits the proliferation and metastasis of ovarian cancer by suppressing LIN28B

Juan Zeng, Yukun Li, Feifei Quan et al. · 2020 · Molecular Medicine Reports

Propofol, a commonly used intravenous anesthetic agent during surgery, has relatively widespread pharmacological actions. Previous studies have reported that propofol may act as an antitumor drug in several cancer types, such as pancreatic…

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

KC-SMARTR: An R package for detection of statistically significant aberrations in multi-experiment aCGH data

Jorma J. de Ronde, Christiaan Klijn, Arno Velds et al. · 2010 · BMC Research Notes

BACKGROUND: Most approaches used to find recurrent or differential DNA Copy Number Alterations (CNA) in array Comparative Genomic Hybridization (aCGH) data from groups of tumour samples depend on the discretization of the aCGH data to gain, loss or no-change states. This causes loss of valuable biological information in tumour samples, which are frequently heterogeneous. We have previously developed an algorithm, KC-SMART, that bases its estimate of the magnitude of the CNA at a given genomic location on kernel convolution (Klijn et al., 2008). This accounts for the intensity of the probe sign

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Classification of Type 2 Diabetes Genetic Variants and a Novel Genetic Risk Score Association With Insulin Clearance

Mark O. Goodarzi, Nicholette D Palmer, Jinrui Cui et al. · 2019 · The Journal of Clinical Endocrinology & Metabolism

CONTEXT: Genome-wide association studies have identified more than 450 single nucleotide polymorphisms (SNPs) for type 2 diabetes (T2D). OBJECTIVE: To facilitate use of these SNPs in future genetic risk score (GRS)-based analyses, we aimed…

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Type 1 diabetes presenting in adults: Trends, diagnostic challenges and unique features

Carmella Evans‐Molina, Richard A. Oram · 2025 · Diabetes Obesity and Metabolism

Type 1 diabetes (T1D) has been historically regarded as a childhood-onset disease; however, recent epidemiological data indicate that adult-onset T1D accounts for a substantial proportion of cases worldwide. There is evidence that…

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Hyaluronic Acid as Macromolecular Crowder in Equine Adipose-Derived Stem Cell Cultures

Sergio Garnica-Galvez, Stefanie Korntner, Ioannis Skoufos et al. · 2021 · Cells

The use of macromolecular crowding in the development of extracellular matrix-rich cell-assembled tissue equivalents is continuously gaining pace in regenerative engineering. Despite the significant advancements in the field, the optimal macromolecular crowder still remains elusive. Herein, the physicochemical properties of different concentrations of different molecular weights hyaluronic acid (HA) and their influence on equine adipose-derived stem cell cultures were assessed. Within the different concentrations and molecular weight HAs, the 10 mg/mL 100 kDa and 500 kDa HAs exhibited the high

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Influence of Statins on Survival Outcome in Patients with Metastatic Castration Resistant Prostate Cancer Treated with Abiraterone Acetate

Martin Boegemann, Katrin Schlack, Ann-Kathrin Fischer et al. · 2016 · PLoS ONE

OBJECTIVE: Even though the exact mechanism is largely unknown until now, statins are supposed to improve survival outcomes in various malignancies. For prostate cancer however, statins are known to compete with dehydroepiandrosterone (DHEAS) for the transport into the cytosol both using the cell by the Solute Carrier Transporter and thus diminish the cellular uptake of DHEAS as a precursor of androgens. Abiraterone inhibits CYP17A1 and thus effectively decreases the production of all relevant androgens including DHEAS. In this study we examined whether statins still affect survival outcome in

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

No Dose-Response Effect of Carbohydrate Mouth Rinse Concentration on 5-km Running Performance in Recreational Athletes

Neil D. Clarke, James R. Thomas, Marion Kagka et al. · 2016 · The Journal of Strength and Conditioning Research

Clarke, ND, Thomas, JR, Kagka, M, Ramsbottom, R, and Delextrat, A. No dose-response effect of carbohydrate mouth rinse concentration on 5-km running performance in recreational athletes. J Strength Cond Res 31(3): 715-720, 2017-Oral…

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Candidate gene studies and the quest for the entrepreneurial gene

Matthijs J. H. M. van der Loos, Philipp Koellinger, Patrick J. F. Groenen et al. · 2011 · Small Business Economics

Candidate gene studies of human behavior are gaining interest in economics and entrepreneurship research. Performing and interpreting these studies is not straightforward because the selection of candidates influences the interpretation of…

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Analysis of Copy Number Variants on Chromosome 21 in Down Syndrome-Associated Congenital Heart Defects

Benjamin L. Rambo‐Martin, Jennifer G. Mulle, David J. Cutler et al. · 2017 · G3 Genes Genomes Genetics

One in five people with Down syndrome (DS) are born with an atrioventricular septal defect (AVSD), an incidence 2000 times higher than in the euploid population. The genetic loci that contribute to this risk are poorly understood. In this study, we tested two hypotheses: (1) individuals with DS carrying chromosome 21 copy number variants (CNVs) that interrupt exons may be protected from AVSD, because these CNVs return AVSD susceptibility loci back to disomy, and (2) individuals with DS carrying chromosome 21 genes spanned by microduplications are at greater risk for AVSD because these microdup

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Gut Microbiota Modulation through Akkermansia spp . Supplementation Increases CAR T-cell Potency

Laura Marcos-Kovandzic, Michele Avagliano, Myriam Ben Khelil et al. · 2025 · Cancer Discovery

This study investigates the clinical relevance of the gut microbiome at taxonomic and metabolic levels in anti-CD19 chimeric antigen receptor (CAR) T-cell therapy, both in patients and in a preclinical syngeneic tumor model. Patients with…

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Significant association between renal function and amyloid-positive area in renal biopsy specimens in AL amyloidosis

Takeshi Kuroda, Naohito Tanabe, Daisuke Kobayashi et al. · 2012 · BMC Nephrology

BACKGROUND: The kidney is a major target organ for systemic amyloidosis that often affects the kidney including proteinura, and elevated serum creatinine (Cr). The correlation between amount of amyloid deposits and clinical parameters is not known. The aim of this study was to clarify correlation the amyloid area in all renal biopsy specimen and clinical parameters. METHODS: Fifty-eight patients with an established diagnosis of AL amyloidosis participated in the study. All patients showed amyloid deposits in renal biopsies. We retrospectively investigated the correlation between clinical data

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Replication of the Association of a MET Variant with Autism in a Chinese Han Population

Xue Zhou, Yang Xu, Jia Wang et al. · 2011 · PLoS ONE

BACKGROUND: Autism is a common, severe and highly heritable neurodevelopmental disorder in children, affecting up to 100 children per 10,000. The MET gene has been regarded as a promising candidate gene for this disorder because it is located within a replicated linkage interval, is involved in pathways affecting the development of the cerebral cortex and cerebellum in ways relevant to autism patients, and has shown significant association signals in previous studies. PRINCIPAL FINDINGS: Here, we present new ASD patient and control samples from Heilongjiang, China and use them in a case-contro

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Lipid nanoparticle screening in nonhuman primates with minimal loss of life

Ryan Zenhausern, Bora Jang, Elisa Schrader Echeverri et al. · 2025 · Nature Biotechnology

Understanding how well delivery in mice predicts delivery in nonhuman primates (NHPs) could make lipid nanoparticle (LNP) discovery more efficient. Yet, few LNP-mRNA drug candidates are tested in NHPs, in part because the experiments…

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

An unbiased comparison of 14 epigenetic clocks in relation to 174 incident disease outcomes

Christos Mavrommatis, Daniel W. Belsky, Kejun Ying et al. · 2025 · Nature Communications

Epigenetic Clocks have been trained to predict chronological age, healthspan and lifespan. Such clocks are often analysed in relation to disease outcomes - typically using small datasets and a limited number of clocks. Here, we present a large-scale (n = 18,859), unbiased comparison of 14 widely used clocks as predictors of 174 incident disease outcomes and all-cause mortality over 10-years of follow up. Second- and third-generation clocks significantly outperform first-generation clocks, which have limited applications in disease settings. Of the 176 Bonferroni significant (P 1%. However, the

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Genome-Wide Association Study of Serum Fructosamine and Glycated Albumin in Adults Without Diagnosed Diabetes: Results From the Atherosclerosis Risk in Communities Study

Stephanie Loomis, Man Li, Nisa M. Maruthur et al. · 2018 · Diabetes

Fructosamine and glycated albumin are potentially useful alternatives to hemoglobin A1c (HbA1c) as diabetes biomarkers. The genetic determinants of fructosamine and glycated albumin, however, are unknown. We performed genome-wide…

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Negative / Null Result ReportBiochemistry, Genetics and Molecular Biology

Significant Differences in Capsid Properties and Potency Between Adeno-Associated Virus Vectors Produced in Sf9 and HEK293 Cells

April R. Giles, Martin Lock, Shu‐Jen Chen et al. · 2023 · Human Gene Therapy

For successful vector-based gene therapy manufacturing, the selected adeno-associated virus (AAV) vector production system must produce vector at sufficient scale. However, concerns have arisen regarding the quality of vector produced…

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Long-term functional and structural outcomes in X-linked retinoschisis: implications for clinical trials

Beau J. Fenner, Jonathan F. Russell, Arlene V. Drack et al. · 2023 · Frontiers in Medicine

Introduction X-linked retinoschisis (XLRS) is an inherited retinal disease (IRD) caused by pathogenic mutations in the retinoschisin gene, RS1 . Affected individuals develop retinal layer separation, leading to loss of visual acuity (VA). Several XLRS gene therapy trials have been attempted but none have met their primary endpoints. An improved understanding of XLRS natural history and clinical outcomes may better inform future trials. Here, we report the long-term functional and structural outcomes of XLRS and the relevance of RS1 genotypes to the visual prognosis of affected individuals. Met

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Comparison of wild-type KT2440 and genome-reduced EM42 Pseudomonas putida strains for muconate production from aromatic compounds and glucose

Caroline R. Amendola, William T. Cordell, Colin M. Kneucker et al. · 2023 · Metabolic Engineering

Pseudomonas putida KT2440 is a robust, aromatic catabolic bacterium that has been widely engineered to convert bio-based and waste-based feedstocks to target products. Towards industrial domestication of P. putida KT2440, rational genome…

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Medium-sized protein language models perform well at transfer learning on realistic datasets

Luiz Carlos Vieira, Morgan L. Handojo, Claus O. Wilke · 2025 · Scientific Reports

Protein language models (pLMs) can offer deep insights into evolutionary and structural properties of proteins. While larger models, such as the 15 billion parameter model ESM-2, promise to capture more complex patterns in sequence space, they also present practical challenges due to their high dimensionality and high computational cost. We systematically evaluated the performance of various ESM-style models across multiple biological datasets to assess the impact of model size on transfer learning via feature extraction. Surprisingly, we found that larger models do not necessarily outperform

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Negative / Null Result ReportBiochemistry, Genetics and Molecular Biology

No Significant Effect of 7,8-Dihydroxyflavone on APP Processing and Alzheimer-Associated Phenotypes

Weitao Zhou, Xiaoyong Li, Daochao Huang et al. · 2015 · Current Alzheimer Research

It is reported that 7,8-dihydroxyflavone (DHF), a TrkB agonist, has beneficial effects on neuronal excitotoxicity, stroke, and Parkinson disease in animal models by enhancing axon regeneration, muscle reinnervation and neuromuscular…

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Residential Proximity to Major Roadways at Birth, DNA Methylation at Birth and Midchildhood, and Childhood Cognitive Test Scores: Project Viva(Massachusetts, USA)

Cheng Peng, Martijn den Dekker, Andrés Cárdenas et al. · 2018 · Environmental Health Perspectives

BACKGROUND: Epigenetic variability is hypothesized as a regulatory pathway through which prenatal exposures may influence child development and health. OBJECTIVE: We sought to examine the associations of residential proximity to roadways at birth and epigenome-wide DNA methylation. We also assessed associations of differential methylation with child cognitive outcomes. METHODS: We estimated residential proximity to roadways at birth using a geographic information system (GIS) and cord blood methylation using Illumina's HumanMethylation450-array in 482 mother-child pairs in Project Viva. We ide

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

MicroRNA expression profiling predicts clinical outcome of carboplatin/paclitaxel-based therapy in metastatic melanoma treated on the ECOG-ACRIN trial E2603

Liza C. Villaruz, Grace Huang, Marjorie Romkes et al. · 2015 · Clinical Epigenetics

BACKGROUND: Carboplatin/paclitaxel (CP), with or without sorafenib, result in objective response rates of 18-20 % in unselected chemotherapy-naïve patients. Molecular predictors of survival and response to CP-based chemotherapy in metastatic melanoma (MM) are critical to improving the therapeutic index. Intergroup trial E2603 randomized MM patients to CP with or without sorafenib. Expression data were collected from pre-treatment formalin-fixed paraffin-embedded (FFPE) tumor tissues from 115 of 823 patients enrolled on E2603. The selected patients were balanced across treatment arms, BRAF stat

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Chronological age, biological age, and individual variation in the stress response in the European starling: a follow-up study

Annie Gott, Clare Andrews, María Larriva et al. · 2018 · PeerJ

) found that a marker of biological age predicted the strength of the stress response even in individuals of the same chronological age. Specifically, birds that had experienced greater developmental telomere attrition (DTA) showed a lower peak corticosterone (CORT) response to an acute stressor, and more rapid recovery of CORT levels towards baseline. Here, we performed a follow-up study using the same capture-handling-restraint stressor in a separate cohort of starlings that had been subjected to a developmental manipulation of food availability and begging effort. We measured the CORT respo

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Comparison of ambulatory capacity and disease progression of Duchenne muscular dystrophy subjects enrolled in the drisapersen DMD114673 study with a matched natural history cohort of subjects on daily corticosteroids

Nathalie Goemans, M. Tulinius, Anna‐Karin Kroksmark et al. · 2016 · Neuromuscular Disorders

Duchenne muscular dystrophy is a rare genetic disorder with life-limiting pathology. Drisapersen induces exon 51 skipping, thereby producing a shorter but functional dystrophin protein. The longest available data are from an open-label…

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Negative / Null Result ReportOpen accessBiochemistry, Genetics and Molecular Biology

Polygenic risk scores in schizophrenia with clinically significant copy number variants

Satoru Taniguchi, Kohei Ninomiya, Itaru Kushima et al. · 2019 · Psychiatry and Clinical Neurosciences

AIMS: Recent studies have revealed that the interplay between polygenic risk scores (PRS) and large copy number variants (CNV; >500kb) is essential for the etiology of schizophrenia (SCZ). To replicate previous findings, including those for smaller CNV (>10kb), the PRS between SCZ patients with and without CNV were compared. METHODS: The PRS were calculated for 724 patients with SCZ and 1178 healthy controls (HC), genotyped using array-based comparative genomic hybridization and single nucleotide polymorphisms chips, and comparisons were made between cases and HC, or between subjects with and

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