e-ISSN: Pending

Browse the failure-mode index

157 real negative results, null findings, and replication failures in Agricultural and Biological Sciences · Negative / Null Result Report. Search the index →

WASTE indexes published research — it does not host or republish full papers. Each entry is a metadata record compiled from open scholarly databases; the abstract is shown in full only where the paper is openly licensed, otherwise a short excerpt under fair use. Classifications are automated and approximate.

Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Harnessing genotype-by-environment interaction to determine adaptability of advanced cowpea lines to multiple environments in Uganda

Abiriga Francis, O. Ongom Patrick, Rubaihayo Patrick et al. · 2020 · Journal of Plant Breeding and Crop Science

This study was conducted to determine the yield stability of advanced cowpea lines in diverse agro-ecological zones of Uganda in order to facilitate documentation requirements for national performance trials (NPT). Thirty cowpea genotypes were evaluated against six checks in three localities, over three growing seasons, making a total of 9 unique environments. The trials were laid in a 6x6 alpha lattice design with three replications and grain yield was the principal trait measured. Single-site and multi-location data were summarized using analysis of variance. Further analysis of stability wa

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Exploring meat analogs: A review of alternative proteins with a focus on food safety and regulatory challenges

Tianyang Zhao, Noemí Echegaray, José M. Lorenzo et al. · 2025 · Food Control

Food safety and environmental concerns are global challenges that require innovative solutions, and alternative protein sources like insect proteins, plant-based meat analogs (PBMAs), and single-cell proteins represent promising pathways toward sustainable nutrition. These proteins are produced through different techniques including extrusion, fermentation, and cell cultivation, with emerging technologies like 3D printing and shear cell structuring enhancing their texture and nutritional quality. Beyond their technological advantages, these alternatives provide significant environmental benefi

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Persistence and Retention of Porcine Reproductive and Respiratory Syndrome Virus in Stable Flies (Diptera: Muscidae)

Kateryn Rochon, Rodney B. Baker, Glen W. Almond et al. · 2015 · Journal of Medical Entomology

We investigated the acquisition of porcine reproductive and respiratory syndrome (PRRS) virus by the stable fly (Diptera: Muscidae; Stomoxys calcitrans (L.)) through a bloodmeal, and virus persistence in the digestive organs of the fly…

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Continental-scale drivers of soil microbial extracellular polymeric substances

Ke Shi, Qing Zheng, Baorong Wang et al. · 2026 · Nature Communications

Abstract Extracellular polymeric substances (EPS) are key microbial residues that contribute to soil organic carbon (SOC) and promote soil aggregation. Yet, their abundance and large-scale controls have only begun to be investigated. We conduct extensive soil sampling across a European transect spanning diverse climates, bedrocks, and land uses. Average soil EPS content is 956 ± 55 µg g -1 soil ( n = 92 sites), with EPS-carbon (EPS-C) contributing 1.6 ± 0.1% to SOC. Bedrock influences EPS content, EPS-C contribution to SOC, and the EPS-C/microbial biomass carbon (MBC) ratio, whereas land use m

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Integrative Genomics and Precision Breeding for Stress-Resilient Cotton: Recent Advances and Prospects

Zahra Ghorbanzadeh, Bahman Panahi, Leila Purhang et al. · 2025 · Agronomy

Developing climate-resilient and high-quality cotton cultivars remains an urgent challenge, as the key target traits yield, fibre properties, and stress tolerance are highly polygenic and strongly influenced by genotype–environment interactions. Recent advances in chromosome-scale genome assemblies, pan-genomics, and haplotype-resolved resequencing have greatly enhanced the capacity to identify causal variants and recover non-reference alleles linked to fibre development and environmental adaptation. Parallel progress in functional genomics and precision genome editing, particularly CRISPR/Cas

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Genomic and phenomic prediction for soybean seed yield, protein, and oil

Liza Van der Laan, Kyle Parmley, Mojdeh Saadati et al. · 2025 · The Plant Genome

Developments in genomics and phenomics have provided valuable tools for use in cultivar development. Genomic prediction (GP) has been used in commercial soybean [Glycine max L. (Merr.)] breeding programs to predict grain yield and seed…

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Trichoderma in Sustainable Agriculture and the Challenges Related to Its Effectiveness

Karina Gutiérrez-Moreno, Ana Isabel Olguín-Martínez, Amelia C. Montoya-Martínez et al. · 2025 · Diversity

Fungi from the genus Trichoderma have been extensively studied and used as biological control agents (BCAs) because of their versatile mechanisms of action. These include triggering systemic resistance, directly inhibiting pathogens, promoting plant growth, enhancing tolerance to abiotic stress, and producing auxins. However, the widespread application of the most studied Trichoderma strains has been limited by discrepancies between their potential results observed in controlled environments and the outcomes in greenhouses and field conditions. These differences are associated with context dep

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

The copepod Acartia tonsa as live feed for fat snook (Centropomus parallelus) larvae from notochord flexion to advanced metamorphosis

Márcia Vanacor Barroso, Cristina Vaz Avelar de Carvalho, Rosemar Antoniassi et al. · 2017 · Latin American Journal of Aquatic Research

From early development until the completion of metamorphosis, fat snook (Centropomus parallelus) larvae are commonly fed the rotifers Brachionus spp. and Artemia spp. nauplii. In this study, cultivated copepods Acartia tonsa were evaluated…

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Emodin and the Anthraquinone Scaffold: Therapeutic Promise and Strategies to Overcome Translational Barriers

Rositsa Mihaylova, Viktoria Elincheva, Rumyana Simeonova et al. · 2026 · Molecules

species, and other medicinal plants, exemplifies the therapeutic potential and translational complexity of the broader anthraquinone scaffold. Anthraquinone derivatives have demonstrated antiproliferative, anti-inflammatory, metabolic, cardiovascular, antifibrotic, and immunomodulatory effects, consistently reported across diverse preclinical models, targeting pathways such as NF-κB, PI3K/AKT, MAPKs, AMPK, PPARs, NLRP3, and ferroptosis-related axes. Despite strong preclinical efficacy, clinical development has been limited by unfavorable absorption, distribution, metabolism, and excretion (ADM

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Exploring consumer intentions to purchase organic food with the health belief model and theory of planned behavior

Shrish Singh, V.K. Jain, Hemraj Verma et al. · 2025 · Discover Food

Food consumption depletes natural resources, pollutes ecosystems, and generates waste, significantly impacting sustainability and the environment. Traditional agriculture, reliant on synthetic fertilizers, pesticides, and excessive water…

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

The beneficial effect of probiotics in the prevention of irinotecan-induced diarrhea in colorectal cancer patients with colostomy: a pooled analysis of two probiotic trials (Probio-SK-003 and Probio-SK-005) led by Slovak Cooperative Oncology Group

Michal Mego, Barbora Judita Kasperová, Jozef Chovanec et al. · 2024 · Frontiers in Oncology

Background: Probiotics could decrease irinotecan-induced diarrhea due to the reduction of intestinal beta-d-glucuronidase activity. This study included a combined analysis of two clinical trials aimed to determine the effectiveness of the probiotics in the prophylaxis of irinotecan-induced diarrhea in metastatic colorectal cancer (CRC) patients. Methods: This combined analysis included 46 patients with CRC enrolled in the Probio-SK-003 (NCT01410955) and 233 patients from Probio-SK-005 (NCT02819960) starting a new line of irinotecan-based therapy with identical eligibility criteria. Patients we

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

A novel strategy to attenuate porcine reproductive and respiratory syndrome virus by inhibiting viral replication in the target pulmonary alveolar macrophages via hematopoietic-specific miR-142

Shaoyuan Tan, Weixin Wu, Xinna Ge et al. · 2023 · One Health Advances

Porcine reproductive and respiratory syndrome virus (PRRSV) is an economically important pathogen for the global pork industry. Although modified live virus (MLV) vaccines are commonly used for PRRSV prevention and control, they still carry a risk of infecting the host and replicating in target cells, thereby increasing the likehood of virus recombination and reversion to virulence. In this study, we inserted the target sequence of miR-142 into the nsp2 hypervariable region of PRRSV to inhibit viral replication in its host cells of pigs, with the aim of achieving virus attenuation. The chimeri

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Bioremediation of Synthetic Dyes by White-Rot Fungi: Enzymatic Mechanisms, Biosorption, and Environmental Applications

Anna Carolina Bruno Ferreira, Ygor Velloso Tavares, Nina Rezende Fontana et al. · 2026 · Molecules

The widespread utilization of synthetic dyes within the textile industry, driven by their chemical recalcitrance and diverse chromatic spectra, constitutes a significant global environmental challenge. Improper discharge of these highly stable effluents into natural water bodies leads to severe ecological imbalances, affecting aquatic life and soil integrity while posing indirect risks to human health due to their mutagenic potential. Conventional physicochemical treatment methods are often hindered by prohibitive operational costs and the frequent generation of hazardous secondary pollutants.

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Probiotics and human health: biological activities, nutritional aspects, immunomodulatory properties, applications, and future perspectives - a comprehensive review

Mohamed T. El‐Saadony, Ahmed Mohamed Saad, Mahmoud Zaki Sitohy et al. · 2026 · Frontiers in Immunology

Probiotics, defined as living microorganisms, are widely recognized for their ability to positively influence the gut microbiota, an effect increasingly linked to a wide array of health benefits. They are claimed to treat or prevent conditions ranging from infant colic to cardiovascular disease, respiratory infections, and certain cancers. Since the beginning of the 21st century, consumer demand for probiotic-enriched foods has risen significantly, propelled by these health assertions. The consumption of such products has been associated with the alleviation of disorders, including irritable b

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Influence of soil properties for improving productivity and grain quality of rainfed rice

Chitra Priyadarshani, Ranjan Laik, Sumit Sow et al. · 2025 · Archives of Agronomy and Soil Science

Integrated crop residue management with micronutrient application can effectively addresses dual challenges of maintaining productivity while enhancing grain nutritional quality of rice in calcareous soils. This study evaluated the role of different soil properties for enhancing yield and grain quality of rice under 29 years of long-term effects of crop residue incorporation and zinc (Zn) fertilization in calcareous Typic Haplaquepts soils. Results showed 100% crop residue incorporation significantly enhanced soil organic carbon (SOC), available nitrogen, available Zn, soil respiration, and we

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Health Effects of Bee Products: A Comprehensive Review

Nevin Sanlier, Elif Yildiz Kaya, Ikbal Irem Yucel · 2026 · Food Science & Nutrition

Honey, bee pollen, propolis, bee bread, royal jelly, bee venom, beeswax, and apilarnil are among the bee-derived products that may serve health-related purposes, as they exhibit various biological activities such as antibacterial, antiviral, antifungal, antioxidant, anti-inflammatory, antitumor, vasodilatory, and blood pressure-lowering effects. In this review, the possible health effects and action mechanisms of bee products frequently used in apitherapy were examined. The therapeutic potential of bee-derived products is attributed to their content of amino acids, fatty acids, carbohydrates,

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Understanding Retailers’ Intentions to Use AI for Product Waste Reduction in Grocery Supply Chains: Extending the Technology Acceptance Model

Kamel Mouloudj, Tiziana Amoriello, Eeman Almokdad et al. · 2026 · Sustainability

Product waste in grocery supply chains remains a major concern for multiple stakeholders, particularly retailers, due to the direct financial losses it generates and the potential risks it poses to customer health and safety. In this context, digital technologies—especially artificial intelligence (AI)—offer promising opportunities to improve retail performance and reduce waste. Accordingly, this study aims to investigate the factors influencing retailers’ intentions to adopt AI-based solutions for product waste reduction. To achieve this objective, the Technology Acceptance Model (TAM) was ex

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Ecology and spread of the North American H5N1 epizootic

Lambodhar Damodaran, Anna S. Jaeger, Louise H. Moncla · 2025 · Nature

Abstract Since late 2021, a panzootic of highly pathogenic H5N1 has devastated wild birds, agriculture and mammals. Here an analysis of 1,818 haemagglutinin sequences from wild birds, domestic birds and mammals reveals that the North American panzootic was driven by around nine introductions into the Atlantic and Pacific flyways, followed by rapid dissemination through wild, migratory birds. Transmission was primarily driven by Anseriformes, while non-canonical species acted as dead-end hosts. In contrast to the epizootic of 2015 (refs. 1,2 ), outbreaks in domestic birds were driven by around

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Mycotoxins and the Intestinal Epithelium: From Barrier Injury to Stem Cell Dysfunction

Wenying Huo, Yingying Qiao, Xiangru He et al. · 2025 · Toxins

Mycotoxins are toxic secondary metabolites produced by filamentous fungi that contaminate agricultural commodities, posing risks to food safety, animal productivity, and human health. The gastrointestinal tract is the first and most critical site of exposure, where the intestinal epithelium functions as both a physical and immunological barrier against luminal toxins and pathogens. While extensive research has demonstrated that mycotoxins disrupt epithelial integrity through tight junction impairment, oxidative stress, apoptosis, and inflammation, their effects on the intestinal stem cell (ISC

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

The impact of large-scale agricultural operations on the grain production resilience: a quasi-natural experiment based on land transfer policy

Da Cui · 2025 · Frontiers in Sustainable Food Systems

Under the dual challenges of intensifying global food security crises and pressing demands for sustainable agricultural development, investigating the mechanisms through which large-scale agricultural operations affect the grain production resilience carries significant strategic importance. Using a quasi-natural experiment based on land transfer policy, this study assesses these mechanisms. The findings indicate that large-scale agricultural operations significantly enhances grain production resilience. This positive effect is mediated by increased income levels among rural residents and grea

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Use of Beauveria bassiana to control northern fowl mites (Ornithonyssus sylviarum) on roosters in an agricultural research facility.

Matthew S W Rassette, Elizabeth I. Pierpont, Tina Wahl et al. · 2011 · PubMed

Treatment of Northern fowl mite (Ornithonyssus sylviarum) infestation on poultry in research facilities can be challenging. The mite has a rapid reproductive cycle (egg to adult in 5 to 7 d), and chemical treatments can be toxic to birds,…

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Agentic AI Framework to Automate Traditional Farming for Smart Agriculture

Muhammad Murad, Muhammad Ahmed, Nizam ul din et al. · 2026 · AgriEngineering

Artificial intelligence (AI) shows great promise for transforming the agriculture sector and can enable the development of many modern farming practices over conventional methods. Nowadays, AI agents and agentic AI have attained popularity due to their autonomous structure and working mechanism. This research work proposes an agentic AI framework that integrates multiple agents developed for farming land to promote climate-smart agriculture and support United Nations (UN) sustainable development goals (SDGs). The developed structure has four agents: Agent A for monitoring soil properties, Agen

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Improving ricotta cheese shelf life using saffron petal extract double nanoemulsions stabilized by plant proteins and Lepidium sativum L. gum

Fereshteh Bakhshalipoor, Marzieh Bolandi, Fariborz Nahidi et al. · 2026 · Scientific Reports

This study evaluated the effects of double nanoemulsions of saffron petal extract (DN-SPE), stabilized with soybean (SPI) and pea protein isolates (PPI), alone or combined with Lepidium sativum L. seed gum (LSG) (SL or PL), on the quality…

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Root traits and mycorrhizal fungi mediate reactive N and warming impacts on soil organic carbon

Yunpeng Qiu, Yunfeng Zhao, Bianbian Wang et al. · 2026 · Nature Communications

Plant roots and arbuscular mycorrhizal fungi (AMF) form a ubiquitous symbiosis in terrestrial ecosystems and critically affect soil organic carbon (SOC) dynamics. However, how roots and AMF mediate the impact of reactive nitrogen (Nr) and…

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Suppression of Cannibalism during Larviculture of Burbot through Size Grading

James M. Barron, Nathan R. Jensen, Paul J. Anders et al. · 2013 · North American Journal of Aquaculture

Abstract The survival and percentage of North American Burbot Lota lota maculosa larvae and metamorphosing larvae presumed to have been cannibalized during a 15-d period immediately following a size-grading event were compared with those of fish in nongraded control groups. In larvae (mean TL, 11.8 mm), grading immediately produced a size distinction, as the group that passed the grader was significantly narrower and shorter than the group retained by the grader. The mean coefficient of variation of the length of larvae in the retained group was significantly lower than that of the control gro

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Leaf ontogeny steers ethylene and auxin crosstalk to regulate leaf epinasty during waterlogging of tomato

Batist Geldhof, Jolien Pattyn, Sam Noppen et al. · 2026 · Plant Stress

Abstract Developing leaves undergo a vast array of age-related changes as they mature. These include physiological, hormonal and morphological changes that determine their adaptation plasticity towards adverse conditions. Waterlogging…

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Emergence of Human-Like Attention in Self-Supervised Vision Transformers: an eye-tracking study

Takuto Yamamoto, Hirosato Akahoshi, Shigeru Kitazawa · 2024 · arXiv

Many models of visual attention have been proposed so far. Traditional bottom-up models, like saliency models, fail to replicate human gaze patterns, and deep gaze prediction models lack biological plausibility due to their reliance on supervised learning. Vision Transformers (ViTs), with their self-attention mechanisms, offer a new approach but often produce dispersed attention patterns if trained with supervised learning. This study explores whether self-supervised DINO (self-DIstillation with NO labels) training enables ViTs to develop attention mechanisms resembling human visual attention.

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Optimization and evaluation of a coarse-grained model of protein motion using X-ray crystal data

Dmitry A. Kondrashov, Qiang Cui, George N. Phillips · 2006 · arXiv

Simple coarse-grained models, such as the Gaussian Network Model, have been shown to capture some of the features of equilibrium protein dynamics. We extend this model by using atomic contacts to define residue interactions and introducing more than one interaction parameter between residues. We use B-factors from 98 ultra-high resolution X-ray crystal structures to optimize the interaction parameters. The average correlation between GNM fluctuation predictions and the B-factors is 0.64 for the data set, consistent with a previous large-scale study. By separating residue interactions into cova

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Disentangling History and Propagation Dependencies in Cross-Subject Knee Contact Stress Prediction Using a Shared MeshGraphNet Backbone

Zhengye Pan, Jianwei Zuo, Jiajia Luo · 2026 · arXiv

Background:Subject-specific finite element analysis accurately characterizes knee joint mechanics but is computationally expensive. Deep surrogate models provide a rapid alternative, yet their generalization across subjects under limited pose and load inputs remains unclear. It remains unclear whether the dominant source of prediction uncertainty arises from temporal history dependence or spatial propagation dependence. Methods:To disentangle these factors, we employed a shared MGN backbone with a fixed mesh topology. A dataset of running trials from nine subjects was constructed using an Open

View details →
Negative / Null Result ReportOpen accessAgricultural and Biological Sciences

Impact of temporal correlations on high risk outbreaks of independent and cooperative SIR dynamics

Sina Sajjadi, Mohammad Reza Ejtehadi, Fakhteh Ghanbarnejad · 2020 · arXiv

We first propose a quantitative approach to detect high risk outbreaks of independent and coinfective SIR dynamics on three empirical networks: a school, a conference and a hospital contact network. This measurement is based on the k-means clustering method and identifies proper samples for calculating the mean outbreak size and the outbreak probability. Then we systematically study the impact of different temporal correlations on high risk outbreaks over the original and differently shuffled counterparts of each network. We observe that, on the one hand, in the coinfection process, randomizat

View details →