CRISPR-Cas9 trans-cleavage is hindered by a flanked R-loop, an elongated spacer, and an inactive HNH domain.
Montagud-Martínez R; Ruiz R; Baldanta S; Delicado-Mateo R; Rodrigo G · 2026 · Nature communications
WASTE classifies this as Failed Experiment Report · AI classification, approximate
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Abstract (excerpt)
Cas9 can process poly(T) single-stranded DNA molecules upon activation in an RNA-guided manner. Here, we uncover key determinants underlying this function. First, we show that unflanked R-loops in the RNA 5' side favor trans-cleavage…
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Metadata source: Europe PMC · DOI 10.1038/s41467-026-68789-3