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Failed Experiment Report

Cross-Genera Amplification of prs/hlyA by Multiplex PCR Resulted in Misidentification of Enterococcus faecium as Listeria monocytogenes

Ali H; Kumurya A; Ajagbe J; Usman Y; Baba AS; Usman M · 2026 · Preprint

WASTE classifies this as Failed Experiment Report · AI classification, approximate

An experimental approach did not work as intended — learn what to avoid before investing the same effort.

Abstract (excerpt)

ABSTRACT Importance Molecular confirmation of Listeria monocytogenes typically employs a multiplex PCR method that targets both genus-specific prs and species-specific hlyA genes. This study assessed the specificity of this assay within…

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Metadata source: Europe PMC · DOI 10.64898/2026.01.20.700535