Directed Evolution Pipeline for the Improvement of Orthogonal Translation Machinery for Genetic Code Expansion at Sense Codons
Wil Biddle; David G. Schwark; Margaret A. Schmitt; John D. Fisk · 2022 · Frontiers in Chemistry
WASTE classifies this as Negative / Null Result Report · AI classification, approximate
The study found no significant effect — useful as a negative control or null benchmark for your own design.
Abstract
The expansion of the genetic code beyond a single type of noncanonical amino acid (ncAA) is hindered by inefficient machinery for reassigning the meaning of sense codons. A major obstacle to using directed evolution to improve the efficiency of sense codon reassignment is that fractional sense codon reassignments lead to heterogeneous mixtures of full-length proteins with either a ncAA or a natural amino acid incorporated in response to the targeted codon. In stop codon suppression systems, missed incorporations lead to truncated proteins; improvements in activity may be inferred from increase
Abstract by Wil Biddle; David G. Schwark; Margaret A. Schmitt; John D. Fisk, Frontiers in Chemistry (2022) — licensed CC BY 4.0.
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Metadata source: OpenAlex · DOI 10.3389/fchem.2022.815788