Microbiome analysis of 940 lung cancers in never-smokers reveals lack of clinically relevant associations
John P. McElderry; Tongwu Zhang; Wei Zhao; Phuc H. Hoang; Samuel Anyaso-Samuel; Jian Sang; Azhar Khandekar; Caleb Hartman · 2025 · Nature Communications
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Abstract
Abstract In spite of the growing interest in the microbiome in human cancer, there are currently only small-scale lung cancer microbiome studies conducted directly on tissue. As part of the Sherlock-Lung study, we studied the microbiomes of 940 lung cancers (4090 samples) in never smokers (LCINS) directly from lung tissue using three data types: 16S rRNA gene sequencing (16S), whole-genome sequencing (WGS) with paired blood, and RNA-seq. We observe very low biomass and few microbiome associations in LCINS using 16S and WGS tissue. Using RNA-seq, we observe more total microbial reads, and decre
Abstract by John P. McElderry; Tongwu Zhang; Wei Zhao; Phuc H. Hoang; Samuel Anyaso-Samuel; Jian Sang; Azhar Khandekar; Caleb Hartman, Nature Communications (2025) — licensed CC BY 4.0.
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Metadata source: DOAJ · DOI 10.1038/s41467-025-66780-y