Positively Charged Residues Are the Major Determinants of Ribosomal Velocity
Catherine A. Charneski; Laurence D. Hurst · 2013 · PLoS Biology
WASTE classifies this as Negative / Null Result Report · AI classification, approximate
The study found no significant effect — useful as a negative control or null benchmark for your own design.
The finding, in one line
“While codon usage is classically considered a major determinant, we find no evidence for this.”
Abstract
Both for understanding mechanisms of disease and for the design of transgenes, it is important to understand the determinants of ribosome velocity, as changes in the rate of translation are important for protein folding, error attenuation, and localization. While there is great variation in ribosomal occupancy along even a single transcript, what determines a ribosome's occupancy is unclear. We examine this issue using data from a ribosomal footprinting assay in yeast. While codon usage is classically considered a major determinant, we find no evidence for this. By contrast, we find that posit
Abstract by Catherine A. Charneski; Laurence D. Hurst, PLoS Biology (2013) — licensed CC BY 4.0.
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Metadata source: OpenAlex · DOI 10.1371/journal.pbio.1001508