Polyprotein Processing as a Determinant for in Vitro Activity of Semliki Forest Virus Replicase
Maija K. Pietilä; Irina C. Albulescu; Martijn J. van Hemert; T. Ahola · 2017 · Viruses
WASTE classifies this as Negative / Null Result Report · AI classification, approximate
The study found no significant effect — useful as a negative control or null benchmark for your own design.
Abstract
Semliki Forest virus (SFV) is an arthropod-borne alphavirus that induces membrane invaginations (spherules) in host cells. These harbor the viral replication complexes (RC) that synthesize viral RNA. Alphaviruses have four replicase or nonstructural proteins (nsPs), nsP1-4, expressed as polyprotein P1234. An early RC, which synthesizes minus-strand RNA, is formed by the polyprotein P123 and the polymerase nsP4. Further proteolytic cleavage results in a late RC consisting of nsP1-4 and synthesizing plus strands. Here, we show that only the late RCs are highly active in RNA synthesis in vitro. F
Abstract by Maija K. Pietilä; Irina C. Albulescu; Martijn J. van Hemert; T. Ahola, Viruses (2017) — licensed CC BY 4.0.
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Metadata source: OpenAlex · DOI 10.3390/v9100292