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11,325 real negative results, null findings, and replication failures in Medicine · Negative / Null Result Report. Search the index →

WASTE indexes published research — it does not host or republish full papers. Each entry is a metadata record compiled from open scholarly databases; the abstract is shown in full only where the paper is openly licensed, otherwise a short excerpt under fair use. Classifications are automated and approximate.

Negative / Null Result ReportOpen accessMedicine

Intermedin inhibits norepinephrine-induced contraction of rat seminal vesicle

P.F. Wong, M.P.L. Cheung, O WS et al. · 2014 · Asian Pacific Journal of Reproduction

Objective: To study the effect of inter medin(IMD) on smooth muscle of rat seminal vesicles including the specific receptors and the signal pathways involved. Methods: The contraction of the seminal vesicle in response to norepinephrine (NE) and ADM2/IMD was studied by the organ bath method. The effects of antagonists for calcitonin gene related peptide (CGRP), adrenomedullin (ADM) and IMD receptors, and inhibitors of nitric oxide synthase, [L-NG-Nitroarginine Methyl Ester, L-NAME) and cAMP-dependent protein kinase (PKA), KT5720] were also investigated. The first overshoot, amplitude, frequenc

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Negative / Null Result ReportMedicine

Child perceptions of note-taking in forensic interviews.

Dang M, Bishop Z, Schick KD et al. · 2026 · Child abuse & neglect

Background In child maltreatment investigations, forensic interviewers must elicit accurate narratives while prioritizing children's wellbeing in a safe environment that minimizes distress. While note-taking may help reduce suggestive…

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Negative / Null Result ReportOpen accessMedicine

Age at diagnosis as a prognostic factor in selected categories of juvenile idiopathic arthritis

Kristiina Aalto, Hannu Kautiainen, Anders Fasth et al. · 2025 · RMD Open

Introduction The age at the onset of juvenile idiopathic arthritis (JIA) can influence the trajectory of the disease. We aimed to clarify how age at the visit 6 months after the onset as a continuous variable affects long-term remission of JIA.Methods This study investigated 358 patients from the Nordic JIA cohort study. Age at diagnosis was analysed continuously. Three age groups were studied: under 3 years, 3–5 years and over 6 years of age. JIA was categorised as oligoarthritis, seronegative polyarthritis and others (enthesitis-related, psoriatic and undifferentiated arthritis). Clinical da

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Negative / Null Result ReportOpen accessMedicine

Analysis of risk factors of rapid attenuation of graft endothelium in the early stage after penetrating keratoplasty.

Ting-Ting Xu, Rui Cao, Yan-Ling Dong et al. · 2022 · PLoS ONE

This study aimed to analyze the factors of rapid attenuation of graft endothelium in the early stage after penetrating keratoplasty (PKP), with a view to guiding patients with PKP to better long-term outcomes. This study included 226 patients who underwent PKP with follow-up time >1 year at the Qingdao Eye Hospital of Shandong First Medical University from January 2018 to June 2020. Medical records were retrospectively studied, and donor factors, patient factors, and surgical factors were comparatively analyzed to clarify those affecting the rapid decay of graft endothelium after PKP. The medi

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Negative / Null Result ReportOpen accessMedicine

Use of Contact Lenses in Eyes with Severe Keratoconus: Long-term Results

Zerrin Tuncer, Turhan Soylu · 2012 · Türk Oftalmoloji Dergisi

Pur po se: To evaluate the long-term results of rigid gas permeable (RGP) contact lenses in severe keratoconic eyes. Ma te ri al and Met hod: Severe keratoconic eyes with RGP contact lenses were evaluated retrospectively. Re sults: Long-term follow-up results of RGP contact lenses applied to 59 eyes of 42 patients (25 women, 17 men) with a diagnosis of severe keratoconus were analyzed. Follow-up period was 3 to 12 years (mean: 6.57±3.60). The mean age at first exam was 25.88±9.10 years, the mean corneal curve K1 was 6.49±0.40 mm and K2 was 5.91±0.40 mm. Mean visual acuities with spectacles and

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Negative / Null Result ReportOpen accessMedicine

In vitro evaluation of the effect of galectins on Schistosoma mansoni motility

Tomoharu Takeuchi, Risa Nakamura, Megumi Hamasaki et al. · 2023 · BMC Research Notes

Abstract Objective Galectins are sugar-binding proteins that participate in many biological processes, such as immunity, by regulating host immune cells and their direct interaction with pathogens. They are involved in mediating infection by Schistosoma mansoni, a parasitic trematode that causes schistosomiasis. However, their direct effects on schistosomes have not been investigated. Results We found that galectin-2 recognizes S. mansoni glycoconjugates and investigated whether galectin-1, 2, and 3 can directly affect S. mansoni in vitro. Adult S. mansoni were treated with recombinant galecti

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Negative / Null Result ReportOpen accessMedicine

Novel fungicide and neonicotinoid insecticide impair flight behavior in pollen foraging honey bees, Apis mellifera

Anna Keodara, Lukas Jeker, Lars Straub et al. · 2024 · Scientific Reports

Abstract Bees are often exposed to pesticides affecting physiological functions and molecular mechanisms. Studies showed a potential link between altered expression of energy metabolism related transcripts and increased homing flight time of foragers exposed to pesticides. In this study, we investigated the effects of thiamethoxam and pyraclostrobin on longevity, flight behavior, and expression of transcripts involved in endocrine regulation (hbg-3, buffy, vitellogenin) and energy metabolism (cox5a, cox5b, cox17) using radio frequency identification (RFID) technology and quantitative polymeras

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Negative / Null Result ReportOpen accessMedicine

Mechanisms of Attenuation of Pulmonary V'O2 Slow Component in Humans after Prolonged Endurance Training.

Jerzy A Zoladz, Joanna Majerczak, Bruno Grassi et al. · 2016 · PLoS ONE

In this study we have examined the effect of prolonged endurance training program on the pulmonary oxygen uptake (V'O2) kinetics during heavy-intensity cycling-exercise and its impact on maximal cycling and running performance. Twelve healthy, physically active men (mean±SD: age 22.33±1.44 years, V'O2peak 3198±458 mL ∙ min-1) performed an endurance training composed mainly of moderate-intensity cycling, lasting 20 weeks. Training resulted in a decrease (by ~5%, P = 0.027) in V'O2 during prior low-intensity exercise (20 W) and in shortening of τp of the V'O2 on-kinetics (30.1±5.9 s vs. 25.4±1.5

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Negative / Null Result ReportOpen accessMedicine

Assessment of Foeniculum Vulgare L. methanolic extract effect on a model of Parkinson’s disease. induced by ICV injection of 6-hydroxydopamin in female rats, using behavioral tests

Hassan Nakisa, Fatemeh Ayoobi, Vahid Ehsani et al. · 2015 · مجله دانشگاه علوم پزشکی بیرجند

Background and Aim: Oxidative stresses are involved in neuronal degeneration of substantia nigra and thereby induction of Parkinson's disease. It is reported that Foeniculum Vulgare L. can affect lactating and modulating dopaminergic system activity and antioxidant activity as well. In the present study, the effects of methanolic extract of Foeniculum Vulgare seeds on a Parkinson’s disease model induced by ICV injection of 6-hydroxydopamin (6-OHDA) in female rats were investigated. Materials and Methods: In this experimental study, 60 female rats were randomly divided into 6 equal groups inclu

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Negative / Null Result ReportOpen accessMedicine

The Effects of Clindamycin and Cefazolin on Osteogenesis of Periodontal Ligament Stem Cells: An In vitro Study

Mohsen Dalband, Mona Isapour, Pantea Nazeman · 2016 · Regeneration, Reconstruction & Restoration

Introduction: Antibiotics are routinely administered in clinical setting for prevention of infections in surgeries and as a routine supplement to the culture medium. The nobility of the present study was to evaluate the effect of two commonly used antibiotics, clindamycin and cefazolin, in dental treatments, on osteogenic capability of periodontal ligament stem cells (PDLSCs). Materials and Methods: PDLSCs were isolated from periodontal ligament (PDL) of the root section out of four healthy extracted teeth. The cells were incubated in the following medium for 28 days: (1) Osteogenic medium (OM

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Negative / Null Result ReportOpen accessMedicine

Intraoperative haloperidol does not improve quality of recovery and postoperative analgesia

Amin Ebneshahidi, Mojtaba Akbari, Masood Mohseni · 2013 · Advanced Biomedical Research

Introduction: Haloperidol has an established role in nausea and vomiting prophylaxis and possible effects on multiple aspects of postoperative recovery including pain and sedation. The purpose of this study was to evaluate the effects of low-dose intraoperative intravenous haloperidol on quality of recovery (QoR) and pain control after general anesthesia and surgery. Methods: Ninety eight American Society of Anesthesiologists (ASA) physical status I-II patients undergoing elective general, gynecologic or orthopedic surgery under general anesthesia were enrolled. Participants were randomly allo

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Negative / Null Result ReportOpen accessMedicine

Assessment of the Physical Activity of Elderly Population of Isfahan, Iran

Sayed Reza Eshaghi, Armindokht Shahsanai, Maryam Mellat Ardakani · 2011 · مجله دانشکده پزشکی اصفهان

Background: Improvement of medical care and decreasing of mortality, have lead to an increase in ageing in Iranian population. The main goal of Active ageing is improving the quality of life. Physical activity is one of the factors that is important to achieve a good quality of life. The aim of this study was to evaluate the physical activity of the elderly in Isfahan. Methods: This cross sectional study was carried out on 350 old residents of Isfahan, Iran between the years 2009-2010. Multi stage cluster sampling technique was used to collect the sample population. Rapid assessment of physica

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Negative / Null Result ReportOpen accessMedicine

The Impact of Previous Childbirth Experiences on Future Childbearing Decisions in Mothers with One Child: A Cross-Sectional Study

Samira Ghorbani, Seyyedeh Solmaz Talebi · 2025 · Journal of Mazandaran University of Medical Sciences

Background and purpose: Declining fertility rates and decreasing population growth are major challenges for the country. Numerous studies have been conducted to identify factors influencing this issue. One such factor is the childbirth experience. This research examines the relationship between previous childbirth experiences and fertility decisions in women with one child. Materials and methods: This cross-sectional study was conducted in 2023-2024 on 400 women with one child covered by health centers in Shahrud. Health centers were selected from various city areas (northern, southern, easter

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Negative / Null Result ReportOpen accessMedicine

Expression of DNA Gyrase at mRNA Levels and Antibacterial Effect of FluoroquinoloneDerived Compounds on Two Antibiotic Resistant Bacteria; Staphylococcus aureus and Enterococcus faecalis

Shokrollah Assar, Ali Darehkordi, Mohammad Moradi et al. · 2019 · Journal of Clinical and Diagnostic Research

Introduction: Fluoroquinolones are a form of antibiotics capable of influencing DNA Gyrase of bacteria. Any alteration in the structure of current fluoroquinolone antibacterials may provide a better effect on the growth inhibition of bacteria. Aim: To assess the probable anti-bacterial activity of two fluoroquinolone derivatives on Staphylococcus aureus (S.aureus) and Enterococcus faecalis (E. faecalis). Materials and Methods: The inhibitory effect of two synthetic derivatives of fluoroquinolones, N-4-methyl (phenyl) -2,2,2- trifluoroacetimidoyl ciprofloxacin (C1), and N-4-methyl (phenyl) -2,2

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Negative / Null Result ReportOpen accessMedicine

The Effect of Sucking Ice Bits on Nausea and Vomiting During Chemotherapy in Patients with Breast Cancer

Mohammad Haddadi, Javad Ganjloo, Hamid Reza Hashemifard et al. · 2019 · Complementary Medicine Journal of Faculty of Nursing and Midwifery

Objective Cancer disease and its treatment methods affect the quality of life of those suffering from breast cancer. Nausea and vomiting are frequent side-effects of chemotherapy. Nowadays, complementary therapies are used along with routine treatments to control this disease. This study aimed to evaluate the effect of sucking ice bits with plain water on nausea and vomiting during chemotherapy in patients with breast cancer. Methods In this clinical trial, participants were 60 breast cancer patients undergoing chemotherapy. They were randomly divided into two groups of control and ice. The ic

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Negative / Null Result ReportOpen accessMedicine

Prognosis of renal re-transplantation for chronic graft failure.

Gong X, Liu Y, Hui F et al. · 2026 · Frontiers in surgery

Objective To analyze the clinical characteristics, patient survival rate, and graft survival rate differences between renal re-transplantation recipients (ReTR) and first-time recipients (FTR) of matched donor kidneys by comparing clinical…

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Negative / Null Result ReportOpen accessMedicine

HBV-related hepatocellular carcinoma susceptibility gene KIF1B is not associated with development of chronic hepatitis B.

Rong Zhong, Yao Tian, Li Liu et al. · 2012 · PLoS ONE

A recent genome-wide association study has identified a new susceptibility locus, kinesin family member 1B gene (KIF1B), strongly associated with progression from chronic hepatitis B (CHB) to hepatitis B virus-related hepatocellular carcinoma (HCC) in Chinese population, this study was carried out to explore the role of the genetic variants in KIF1B in the development of chronic hepatitis B.Three KIF1B polymorphisms (rs8019, rs17401924, and rs17401966) were selected and genotyped in 473 CHB patients and 580 controls with no history of CHB. Odds ratios (ORs) and 95% confidence intervals (CIs) w

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Negative / Null Result ReportOpen accessMedicine

Effects of hot air treatment and chitosan coating on citric acid metabolism in ponkan fruit during cold storage.

Yang Gao, Chaonan Kan, Chunpeng Wan et al. · 2018 · PLoS ONE

In citrus fruit, citric acid is the predominant organic acid which influence fruit taste, flavor and quality. The effect of hot air treatment (HAT 40°C, 48 h) and 1.0% chitosan coating on the change of organic acids and the related gene expression of citric acid synthesis and degradation in ponkan (Citrus reticulata Blanco) fruit during cold storage have been studied. The results showed that citric acid was the main organic acid in fruit, the trend change of citric acid content was consistent with total organic acids and titratable acidity (TA) content, which decreased with the prolongation of

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Negative / Null Result ReportOpen accessMedicine

Systemic blockade of dopamine D2-like receptors increases high-voltage spindles in the globus pallidus and motor cortex of freely moving rats.

Chen Yang, Shun-Nan Ge, Jia-Rui Zhang et al. · 2013 · PLoS ONE

High-voltage spindles (HVSs) have been reported to appear spontaneously and widely in the cortical-basal ganglia networks of rats. Our previous study showed that dopamine depletion can significantly increase the power and coherence of HVSs in the globus pallidus (GP) and motor cortex of freely moving rats. However, it is unclear whether dopamine regulates HVS activity by acting on dopamine D₁-like receptors or D₂-like receptors. We employed local-field potential and electrocorticogram methods to simultaneously record the oscillatory activities in the GP and primary motor cortex (M1) in freely

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Negative / Null Result ReportOpen accessMedicine

A cephalometric study of nasopharynx in skeletal class I and III malocclusion

Mohamad Hosein Toodeh Zaeim, Soghra Yassaei, Hosein Aghili et al. · 2008 · Iranian Journal of Orthodontics

Aim: Nasopharyngeal space is estimated from a point on the posterior part of the soft palate till the nearest point on the wall of the pharynx and the aim of the present study was to study the nasopharyngeal indices in nasal breathers with class I and III malocclusions.Materials and Methods: This was a descriptive analytical study done by the case - control method on 35 lateral cephalometries of patients with skeletal Class III malocclusion as the study group and 30 lateral cephalometries of patients with skeletal Class I malocclusion as the control group. The population under study included p

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Negative / Null Result ReportOpen accessMedicine

Effect of Motor Cortex Transcranial Direct Current Stimulation (tDCS)on the Strength and Endurance performance of Healthy Collegiate Subjects

Sirous Nazari pirdoosti, Vahid Valipour dehnou, Ehsan Gharamanlou · 2019 · Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām

Introduction: Transcranial direct current stimulation (tDCS) is a non-invasive technique used to modify the activity of the cortex of the brain. This form of current is capable of inducing changes in the electrical activity inside and outside of the neurons, which leads to a change in the resting membrane potential and thus improves the efficiency of the neural synapse. Therefore, the aim of this study was to investigate the effect of tDCS over motor cortex on strength and endurance performance of healthy collegiate subjects. Materials & Methods: In total, 10 young and healthy male subjects (a

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Negative / Null Result ReportOpen accessMedicine

Effects of elevated [CO2] and low soil moisture on the physiological responses of Mountain Maple (Acer spicatum L.) seedlings to light.

Gabriel Danyagri, Qing-Lai Dang · 2013 · PLoS ONE

Global climate change is expected to affect how plants respond to their physical and biological environments. In this study, we examined the effects of elevated CO2 ([CO2]) and low soil moisture on the physiological responses of mountain maple (Acer spicatum L.) seedlings to light availability. The seedlings were grown at ambient (392 µmol mol(-1)) and elevated (784 µmol mol(-1)) [CO2], low and high soil moisture (M) regimes, at high light (100%) and low light (30%) in the greenhouse for one growing season. We measured net photosynthesis (A), stomatal conductance (g s), instantaneous water use

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Negative / Null Result ReportOpen accessMedicine

Effects of the small molecule HERG activator NS1643 on Kv11.3 channels.

Arne Bilet, Christiane K Bauer · 2012 · PLoS ONE

NS1643 is one of the small molecule HERG (Kv11.1) channel activators and has also been found to increase erg2 (Kv11.2) currents. We now investigated whether NS1643 is also able to act as an activator of Kv11.3 (erg3) channels expressed in CHO cells. Activation of rat Kv11.3 current occurred in a dose-dependent manner and maximal current increasing effects were obtained with 10 µM NS1643. At this concentration, steady-state outward current increased by about 80% and the current increase was associated with a significant shift in the voltage dependence of activation to more negative potentials b

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Negative / Null Result ReportOpen accessMedicine

Dynamics of tropomyosin in muscle fibers as monitored by saturation transfer EPR of bi-functional probe.

Roni F Rayes, Tamás Kálai, Kálmán Hideg et al. · 2011 · PLoS ONE

The dynamics of four regions of tropomyosin was assessed using saturation transfer electron paramagnetic resonance in the muscle fiber. In order to fully immobilize the spin probe on the surface of tropomyosin, a bi-functional spin label was attached to i,i+4 positions via cysteine mutagenesis. The dynamics of bi-functionally labeled tropomyosin mutants decreased by three orders of magnitude when reconstituted into "ghost muscle fibers". The rates of motion varied along the length of tropomyosin with the C-terminus position 268/272 being one order of magnitude slower then N-terminal domain or

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Negative / Null Result ReportOpen accessMedicine

Cluster Randomized Controlled Trial Evaluation of a Gender Equity and Family Planning Intervention for Married Men and Couples in Rural India.

Anita Raj, Mohan Ghule, Julie Ritter et al. · 2016 · PLoS ONE

Background Despite ongoing recommendations to increase male engagement and gender-equity (GE) counseling in family planning (FP) services, few such programs have been implemented and rigorously evaluated. This study evaluates the impact of CHARM, a three-session GE+FP counseling intervention delivered by male health care providers to married men, alone (sessions 1&2) and with their wives (session 3) in India. Methods and findings A two-armed cluster randomized controlled trial was conducted with young married couples (N = 1081 couples) recruited from 50 geographic clusters (25 clusters randomi

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Negative / Null Result ReportOpen accessMedicine

Toxicity of Parasporin-4 and Health Effects of Pro-parasporin-4 Diet in Mice

Shiro Okumura, Hironori Koga, Kuniyo Inouye et al. · 2014 · Toxins

Parasporin-4 (PS4) is an aerolysin-type β-pore-forming toxin produced by Bacillus thuringiensis strain A1470. It exhibits specific cytotoxicity against human cancer cell lines; therefore, it is expected to be useful for the diagnosis and treatment of particular types of cancer cells. We examined the acute toxicity of PS4 on ICR mice. The LD50 value was 160 μg/kg by a subcutaneous route. Potassium, ammonium, magnesium ion, creatinine, and urea nitrogen decreased in urine by the injection of PS4. Simultaneously, creatinine and urea nitrogen in mice serum increased. These results imply that PS4 i

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Negative / Null Result ReportOpen accessMedicine

Development of Emotion Word Comprehension in Chinese Children from 2 to 13 Years Old: Relationships with Valence and Empathy.

Yanwei Li, Dongchuan Yu · 2015 · PLoS ONE

Children's emotion word comprehension (EWC) has constantly received a great deal of attention in developmental science. However, since previous reports focused on only English emotion vocabulary, researchers thus far remained unclear as to the developmental trajectories of EWC (to Chinese emotion words) in Chinese children, let alone the cross-cultural difference of EWC in different languages (i.e., English V.S. Chinese). Furthermore, the influence of valence on EWC, as well as the interaction mechanism between EWC and empathy, has not been fully investigated. Finding answers to these research

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Negative / Null Result ReportOpen accessMedicine

Comparative Effectiveness of Laterally Placed Expandable versus Static Interbody Spacers: A 1-Year Follow-Up Radiographic and Clinical Outcomes Study

Yan Michael Li, Richard Francis Frisch, Zheng Huang et al. · 2021 · Asian Spine Journal

Study Design Retrospective chart review. Purpose This study compared the clinical and radiographic outcomes of patients treated with expandable and static interbody spacers following minimally invasive lateral lumbar interbody fusion (MIS-LLIF) with 12-month follow-up. Overview of Literature A common surgical option for the treatment of degenerative disk disease (DDD) is MIS-LLIF using static or expandable spacers to restore disk height (DH), neuroforaminal height (NH), and segmental lordosis. Static spacers may require excessive trialing and aggressive impaction, potentially leading to endpla

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