Dehydration-based preservation of cat cumulus–oocyte complexes is not improved by membrane-permeable trehalose
Pei-Chih Lee; Yuqing Yang; Kaywalee Chatdarong; Pierre Comizzoli · 2026 · Frontiers in Veterinary Science
WASTE classifies this as Negative / Null Result Report · AI classification, approximate
The study found no significant effect — useful as a negative control or null benchmark for your own design.
Abstract
IntroductionDry-preservation of gametes offers a promising alternative to cryobanking by potentially storing cells at ambient temperatures; however, successful preservation of whole oocytes remains limited by dehydration-induced cellular damages. This study evaluated whether intracellular delivery of trehalose via a membrane-permeable derivative, trehalose hexaacetate (6-O-Ac-Tre), could enhance dehydration tolerance of domestic cat cumulus–oocyte complexes (COCs), a relevant model for mammalian fertility preservation.MethodsCat COCs were incubated with 0, 3, 10, or 30 mM of 6-O-Ac-Tre to asse
Abstract by Pei-Chih Lee; Yuqing Yang; Kaywalee Chatdarong; Pierre Comizzoli, Frontiers in Veterinary Science (2026) — licensed CC BY 4.0.
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Metadata source: DOAJ · DOI 10.3389/fvets.2026.1805930