Induction of somatic embryogenesis and cryopreservation of Abies pinsapo Boiss.
Laura Cabero-Moreno, Ignacio Landeras-López, María Victoria Bravo-Navas et al. · 2025 · Frontiers in Plant Science
Abies pinsapo is an endangered species, endemic to southern Spain. Somatic embryogenesis and cryopreservation constitute important biotechnological tools, which can be used in order to improve the management and conservation of threatened species. The objective of this work was to develop somatic embryogenesis and cryopreservation protocols for A. pinsapo. Somatic embryogenesis was induced from mature zygotic embryos of A. pinsapo cultured on solid MS medium with macroelements at half-strength and supplemented with 20 g L-1 sucrose and 5 mg L-1 6-benzylaminopurine (BA). Embryogenic cultures su
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